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| There are 16 papers published in subject: since this site started. | |||
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| 1. Simultaneous extraction of baicalin and saccharides from Scutellariae Radix with aqueous two-phase system | |||
| Yuan Xiaolin,Mu Xiaojing,Li Yan,Gou Yan,Tang Meiling,Xiao Shangyou | |||
Traditional Chinese Medicine and Pharmacology 27 April 2020
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| Abstract:Scutellariae Radix is a Chinese herb for detoxification. Efficient extraction of its main active components baicalin and saccharides is interesting to pharmaceutical and cosmetic fields. The baicalin and saccharides were efficiently extracted with aqueous two-phase systems (ATPSs) Ethanol-(NH4)2SO4-H2O (EtSO) and Ethanol-cosolvent-(NH4)2SO4-H2O (EtSO-cosolvent). The factors such as pH, (NH4)2SO4 concentration, ethanol fraction, cosolvent fraction, extraction time and temperature were optimized and thermodynamics for partition was discussed. With the extractions at 48 C for 40 min, the EtSO, EtSO-MtOH and EtSO-Acetone exhibited higher baicalin yield than conventional 70% ethanol system (v/v) by 21.4%, 24.8% and 26.2%, respectively; and they displayed saccharides yield in a similar level to that with water system. By dialysis method the saccharides extracted with ATPS was proved to have similar molecular weight to those extracted with water. Therefore, it is feasible to obtain two birds (two targets) with one stone (ATPS) in high efficiency. | |||
| TO cite this article:Yuan Xiaolin,Mu Xiaojing,Li Yan, et al. Simultaneous extraction of baicalin and saccharides from Scutellariae Radix with aqueous two-phase system[OL].[27 April 2020] http://en.paper.edu.cn/en_releasepaper/content/4751820 | |||
| 2. Pharmacokinetics of Terminalia Phenolics in Rats after Oral Administration of a Mongolian Compound Medicine Alatan-5 | |||
| Wu Dandan,Azzaya Jukov,Ma Chaomei | |||
| Traditional Chinese Medicine and Pharmacology 04 May 2017
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| Abstract:This paper reports the pharmacokinetic properties of Terminalia phenolics after administration of Alatan-5 which is a compound Mongolian medicine containing the fruit of T. chebula in combination with four other medicines. Gallic acid, ellagic acid, casuarinin, and chebulic acid were detected and quantified in the rat plasma. Gallic acid showed the largest AUC value among these four constituents when Alatan-5 was administrated, and its AUC value was larger than when T. chebula fruit was used alone. While the AUC values of the other constituents were smaller when Alatan-5 was used than when T. chebula fruit was administrated alone. These data indicated that gallic acid and other Terminalia phenolics could play important roles in the pharmacologic effects of Alatan-5 and that other medicines in this compound Mongolian medicine could greatly alter the pharmacokinetic behaviors of Terminalia phenolics. | |||
| TO cite this article:Wu Dandan,Azzaya Jukov,Ma Chaomei. Pharmacokinetics of Terminalia Phenolics in Rats after Oral Administration of a Mongolian Compound Medicine Alatan-5[OL].[ 4 May 2017] http://en.paper.edu.cn/en_releasepaper/content/4732391 | |||
| 3. The effects of the composition of the effective components of FuFang Danshen in Alzheimer's disease mice induced by aluminum trichloride and d-galactose | |||
| LIANG Chengjie,CHEN Chen,YANG Jie | |||
| Traditional Chinese Medicine and Pharmacology 15 November 2016
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| Abstract:Alzheimer's disease (AD) is a chronic neurodegenerative disease. There is still no cure, but there are medicines in clinical use. The traditional Chinese medicine (TCM) for clinical treatment of AD has become a research trend. TCM's composition is more competitive than TCM's compound in the international market because it is similar to the western medicine compound. Fufang Danshen (FFDS)'s composition (including Tanshinone IIA, Notoginsenoside R1 and borneol) for AD's treatment has not been repoted up to now. Our purpose was to explain whether the FFDS' composition improves learning and memory ability based on a mouse model indued by a combination of d-galactose (D-gal) and aluminum muriate (AlCl3). AlCl3 and D-gal were administrated into mice through intraperitoneal injection for 90 days to build AD model, and then Huperzine A and FFDS' composition were used for treatment by intragastric administration for 2 weeks. Memory function was evaluated using Morris water maze (MWM). The subcellular structure of neurons, namely Nissl bodies, was analysed by Nissl staining. ELISA assay was used to assess the levels of INF-γ and IL-4 in serum. MWM test reveals that FFDS' composition can repair the learning and memory impairment of AD mice caused by long-term intake of AlCl3 and D-Gal. ELISA assay shows that FFDS' composition can significantly decrease the serum levels of INF-γ in AD mice, and relieve the inflammation reaction related to INF-γ. Nissl staining results show FFDS' composition can obviously improve the morphology of the neuron. Our results support the use of traditional medicinal herbs containing FFDS' composition for neuroprotection and memory loss. | |||
| TO cite this article:LIANG Chengjie,CHEN Chen,YANG Jie. The effects of the composition of the effective components of FuFang Danshen in Alzheimer's disease mice induced by aluminum trichloride and d-galactose[OL].[15 November 2016] http://en.paper.edu.cn/en_releasepaper/content/4710335 | |||
| 4. Wuling San ameliorated hypothalamic inflammation to improve insulin signaling defect in fructose-fed mice | |||
| Yang Ying,Zhang Dongmei,Jiao Ruiqing,Kong Lingdong,Li Jianmei | |||
| Traditional Chinese Medicine and Pharmacology 25 May 2016
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| Abstract:Fructose excess consumption induces insulin resistance-associated metabolic diseases. Hypothalamic insulin signaling plays a pivotal role in controlling whole-body insulin sensitivity and energy homeostasis. Wuling San and its modifications exerted various effects including anti-hypertension, anti-inflammation, anti-dyslipidemia and anti-insulin resistance. In this study, we investigated its regulatory effects on the hypothalamus of high fructose-fed mice. Mice were fed 30% fructose in drinking water for 12 weeks. After 6 weeks, these animals were orally treated with Wuling San (987, 1316, 1755 and 2340 mg/kg), allopurinol (5 mg/kg) and water daily for the next 6 weeks, respectively. Wuling San ameliorated fructose-induced hypertension, hyperlipidemia, and hyperinsulinemia and insulin resistance in mice. Wuling San ameliorated high fructose-caused hypothalamic inflammation in mice by suppressing the activation of hypothalamic nuclear factor κB (NF-κB) pathway and NOD-like receptor 3 (NLRP3) inflammasome. Wuling San also effectively restored high fructose-induced hypothalamic insulin signaling defect by up-regulating the phosphorylation of insulin receptor and protein kinase B. Allopurinol had similar effects. These results provide in vivo evidence that Wuling San-mediated inhibition of NF-κB pathway/NLRP3 inflammasome activation in the hypothalamus of mice may be associated with the reduction of hypothalamic inflammatory lesions, contributing to the improvement of hypothalamic insulin signaling defect in this model. Thus, Wuling San with the central activity may be a therapeutic for high fructose-induced metabolic syndrome in humans. | |||
| TO cite this article:Yang Ying,Zhang Dongmei,Jiao Ruiqing, et al. Wuling San ameliorated hypothalamic inflammation to improve insulin signaling defect in fructose-fed mice[OL].[25 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4693227 | |||
| 5. To Unveil the Molecular Mechanisms of Qi and Blood of Traditional Chinese Medicine through Systems Biology-Based Investigation into Si-Jun-Zi-Tang and Si-Wu-Tang formulae | |||
| Zhang Li,Sun Jing,Kun Zhang,Wu Liping,Fan Yongsheng,Xie Zhijun | |||
| Traditional Chinese Medicine and Pharmacology 04 April 2016
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| Abstract:[Object]To unveil potential biologic mechanisms between Qi and blood through analysis of Si-Wu-Tang (SWT) and Si-Jun-Zi-Tang (SJZT) with biomedical approaches. [Methods]Ingredients of eight herbs from SWT and SJZT were retrieved from TCMID database. The genes targeted by these ingredients were chosen according to their STITCH defined high confident (combined-score more than 0.7). We then performed enrichment analysis by Gene Ontology (GO) and DAVID. Enriched GO terms with p-value less than 0.01 were collected and interpreted the biological meanings through DAVID and KEGG. Then, a visualized network was constructed with ClueGO (a Cytoscape plug-in) to compare the difference pathways between SWT and SJZT formulae. [Results] A total of 243 genes targeted by 195 ingredients of SWT formula and 209 genes targeted by 61 ingredients of SJZT were obtained by retrieving from TCMID database. Six metabolism pathways and two environmental information processing pathways enriched by targets were correlated with two or more herbs in SWT and SJZT formula, respectively. Expect for disease associated pathways, SWT significantly influence amino acid and carbohydrate metabolism. While pathways enriched by genes targeted by ingredients of SJZT mainly belonged to organismal system function, environmental information processing, metabolism and cellular processes. The common pathways of SWT and SJZT belonged to organismal systems, metabolism, environmental information processing and cellular processes. [Conclusion]SWT with the functions of influencing amino acid and carbohydrate metabolism is significant different from SJZT with the actions of influencing neuroendocrine system by affecting serotonergic synapse to regulate thyroid hormone, ovarian steroidogenesis, prolactin and oxytocin secrction and the actions of regulating corrective hormones secretion and promoting vascular smooth muscle contraction. The common effects of SWT and SJZT are regulating the functional activities of the hypothalamic pituitary adrenal and gonadal axis by affect inhibitory synapses (GABAergic synapse) to stimulate estrogen and steroid hormones secretion and strengthening the ability of anti-infection. All the different and common pathways also reflect the characteristics of blood deficiency and Qi deficiency and indirectly reflect the molecular mechanism of blood and Qi of TCM.) | |||
| TO cite this article:Zhang Li,Sun Jing,Kun Zhang, et al. To Unveil the Molecular Mechanisms of Qi and Blood of Traditional Chinese Medicine through Systems Biology-Based Investigation into Si-Jun-Zi-Tang and Si-Wu-Tang formulae[OL].[ 4 April 2016] http://en.paper.edu.cn/en_releasepaper/content/4682229 | |||
| 6. Preparation, hydrolysis and bioactivity evaluation of a natural polysaccharide from sclerotium of Poria cocos | |||
| Chen Qi,Yang Jia,Xu Wenjin | |||
| Traditional Chinese Medicine and Pharmacology 08 March 2016
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| Abstract:Pachyman isolated from a natural polysaccharide is lack of bioactivity due to its water-insolubility. In this study, in order to improve its bioactivity, pachyman was enzymatically hydrolyzed to obtain water soluble pachyman (WSP). Moreover, in order to further evaluate the relationship between the structure and bioactivity of pachyman, carboxymethylated pachyman (CMP) with different degree of substitution (DS) and water-soluble pachyman with different molecular weight were also prepared and their antitumor activities against S180 cells were further evaluated. Our results showed that both enzymatic hydrolysis and chemical modification were effective for improving the antitumor activity of pachyman. However, our data also suggested that the molecular weight and the degree of substitution of the derivatives should be taken into account when developing polysaccharide-based drugs. | |||
| TO cite this article:Chen Qi,Yang Jia,Xu Wenjin. Preparation, hydrolysis and bioactivity evaluation of a natural polysaccharide from sclerotium of Poria cocos[OL].[ 8 March 2016] http://en.paper.edu.cn/en_releasepaper/content/4679252 | |||
| 7. Icariin protects SH-SY5Y cells from formaldehyde-induced injury through inhibition of tau phosphorylation | |||
| Song Yixiang,Miao Junye,Qiang Min,He Rongqiao,Wang Xuemei,Li Weiwei | |||
| Traditional Chinese Medicine and Pharmacology 20 March 2014
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| Abstract:BACKGROUND: Formaldehyde-induced neurotoxicity is implicated in the pathology of Alzheimer's disease. Icariin, a flavonoid found in Chinese herbal medicine Epimedium, exhibits neuroprotective activity; however, whether Icariin antagonizes formaldehyde-induced nerve injury is unclear. AIMS: To investigate the neuroprotective effects of Icariin on formaldehyde-treated SH-SY5Y cells and the possible mechanisms involved. METHODS: SH-SY5Y cells were divided into control group, formaldehyde treatment group, and Icariin treatment group. Cell viability, apoptosis, and morphological changes were determined by CCK8, flow cytometry, and confocal microscopy, respectively. The phosphorylation of protein tau was examined by Western blot. RESULTS: Formaldehyde showed a half lethal dose (LD50) of 0.3 mM in SH-SY5Y cells. Icariin (1 - 10 μM) prevented formaldehyde-induced cell death in SH-SY5Y cells in a dose-dependent manner, with the optimal effect observed at 5 μM. Examination of cell morphology by confocal microscopy demonstrated that 5 μM ICA significantly attenuated formaldehyde-induced cell injury. Additionally, Icariin inhibited formaldehyde-induced cell apoptosis in SH-SY5Y cells. Results from western blot analysis showed that Icariin suppressed formaldehyde-induced tau phosphorylation at Thr181 and Ser396, while having no effect on the total tau protein level. Furthermore, Icariin reduced Tyr216 phosphorylation and increased Ser9 phosphorylation of the tau kinase GSK-3β. CONCLUTION: Icariin protects SH-SY5Y cells from formaldehyde-induced injury possibly through inhibition of GSK-3β-mediated tau phosphorylation.????? | |||
| TO cite this article:Song Yixiang,Miao Junye,Qiang Min, et al. Icariin protects SH-SY5Y cells from formaldehyde-induced injury through inhibition of tau phosphorylation[OL].[20 March 2014] http://en.paper.edu.cn/en_releasepaper/content/4590573 | |||
| 8. Neuroprotective effects of extract of Acanthopanax senticosus harms on WT-α-Syn or A53T-α-Syn transgenic SH-SY5Y cells | |||
| LI Xuzhao,WANG Kexin,ZHANG Shuainan,YANG Zhiming,LIU Hong-yu,LU Fang,Liu Shumin | |||
| Traditional Chinese Medicine and Pharmacology 29 July 2013
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| Abstract:AIM: Extract of Acanthopanax senticosus harms (EAS) has been shown to have neuroprotective effects on dopaminergic neurons in Parkinson's disease (PD) mice model. α-Synuclein is a key player in the pathogenesis of PD, the elevated level of which is deleterious to dopaminergic neurons, and enhancing its clearance might be a promising strategy for treating PD. To assess the potential of EAS in this regard, we investigated its effect on the SH-SY5Y cells overexpressing wild-type α-synuclein (WT-α-Syn) or A53T mutant α-synuclein (A53T-α-Syn) and the implicated pathway it might mediate. METHODS: After treatment with EAS, the effect of EAS on apoptosis in these two transgenic cells was analyzed by flow cytometry. The changes of α-synuclein, caspase-3, parkin, phospho-protein kinase B (Akt), phospho-glycogen synthase kinase 3 beta (GSK3β), and phospho-microtubule-associated protein tau (Tau) in WT-α-Syn or A53T-α-Syn transgenic cells were analyzed by western blotting and quantitative real-time PCR. RESULTS: The changes of α-synuclein, caspase-3, parkin, phospho-Akt, phospho-GSK3β, and phospho-Tau in WT-α-Syn or A53T-α-Syn transgenic cells were reverted back to near normal levels. CONCLUSION: The neuroprotective effects of EAS may be able to protect WT-α-Syn or A53T-α-Syn transgenic SH-SY5Y cells from α-synuclein overexpression and toxicity. Therefore, we speculate that EAS might be a promising candidate for prevention or treatment of α-synuclein-related neurodegenerative disorders such as PD. | |||
| TO cite this article:LI Xuzhao,WANG Kexin,ZHANG Shuainan, et al. Neuroprotective effects of extract of Acanthopanax senticosus harms on WT-α-Syn or A53T-α-Syn transgenic SH-SY5Y cells[J]. | |||
| 9. Simultaneous determination of seven lignans in Justicia procumbens by high performance liquid chromatography- photodiode array detection using relative response factors | |||
| LUO Zuliang,KONG Weijun,QIU Feng,YANG Meihua,LI Qian,WEI Riwei,YANG Xiaoli,QIN Jieping | |||
| Traditional Chinese Medicine and Pharmacology 13 September 2012
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| Abstract:A simple and sensitive high performance liquid chromatography coupled with photodiode array (HPLC-PDA) method was developed for simultaneous determination of 7 lignans in Justicia procumbens using relative response factors. The chromatographic separation was performed on a Shiseido Capcell Pak C18 column (250 × 4.6 mm id, 5 μm), a gradient elution of acetonitrile/water and a photodiode array detector. The column temperature was maintained at 35 C and the detection wavelength was set at 256 nm. Chinensinaphthol methyl ether was selected as the reference compound for calculating the relative response factors of the lignans. It has shown that the relative response factors (RRFs) for lignans are quite similar at 256 nm of detection under different analytical conditions (different columns and different HPLC instruments). Using RRFs, no lignan is needed as a reference standard, making the method ideal for rapid, routine analysis, especially for those laboratories where lignans standards are not readily available. An economic and practicable HPLC method using RRFs was established for the determination of seven lignans in J. procumbens. This method not only can determine multiple indexes in Chinese materia medica (CMM) simultaneously, but also resolve the problem of lacking of chemical standards. It will be a good quality evaluation method and pattern for CMM. | |||
| TO cite this article:LUO Zuliang,KONG Weijun,QIU Feng, et al. Simultaneous determination of seven lignans in Justicia procumbens by high performance liquid chromatography- photodiode array detection using relative response factors[J]. | |||
| 10. Effect of solanine on cell apoptosis by regulating Bcl-2/Bax ratio in HepG2 cell | |||
| JI Yubin,GAO Shi-yong | |||
| Traditional Chinese Medicine and Pharmacology 10 January 2012
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| Abstract:Objective To explore the anti-tumor effect of solanine and the mechanisms involved. Methods The in vivo anti-tumor effect of solanine is observed using models developed through in vivo transplantation of tumor cells; in vitro lines of sensitive anti-tumor cells are selected from the digestive system using MTT assay; the effect of solanine on cell morphology is observed using transmission electronic microscopy; the morphology of apoptotic cells is observed using Annexin V/PI double staining and laser confocal scanning microscopy; the rate of cell apoptosis is measured using Annexin V/PI double staining and flow cytometry; the concentration of intracellular Ca2+ is determined using Fluo-3/AM staining and laser confocal scanning microscopy; the membrane potential of cellular mitochondria is determined using TMRE staining and laser confocal scanning microscopy; the protein expression of Bcl-2 and Bax is measured using immunological marking and laser confocal scanning microscopy; and the activity of caspase-3 is measured using the colorimetric method. Results Solanine can inhibit the growth of tumor weight in S180 tumor-bearing mice and prolong the survival time of H22 tumor-bearing mice. Selection by MTT assay reveals that HepG2 cells are quite sensitive to solanine, for solanine can induce morphological changes characteristic of apoptotic cells in HepG2 cells, with the rate of early apoptosis being 4%, 8.5%, and 20.1%, for HepG2 cells treated for 24 h with solanine of concentrations of 0.4, 2, and 10μg, respectively. Solanine can raise the concentration of intracellular Ca2+ and lower the membrane potential. It can reduce the protein expression of Bcl-2 while increasing that of Bax, thus increasing the activity of caspase-3. Conclusion Solanine demonstrates clear anti-tumor activity, inhibiting human hepatocarcinoma. This inhibitory effect is achieved through solanine’s decreasing the Bcl-2/Bax ratio, thus increasing the concentration of intracellular Ca2+, which turns on the enzymatic activity of the caspase family, thus inducing the apoptosis of HepG2 cells. | |||
| TO cite this article:JI Yubin,GAO Shi-yong. Effect of solanine on cell apoptosis by regulating Bcl-2/Bax ratio in HepG2 cell[OL].[10 January 2012] http://en.paper.edu.cn/en_releasepaper/content/4458765 | |||
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