Abstract:In this present work, a simple regenerable electrochemical aptasensor for the parallel and continuous detection of protein biomarkers was reported. Mucin 1 protein (MUC1) and cinoembryonic antigen (CEA) were employed as the models of protein biomarkers. The sensing interface was modified by MUC1 aptamer and methylene blue (MB)-modified CEA aptamer via hybridization with a linker DNA. Herein, MB served as a signal transducer, MUC1 and CEA were parallel measured, and the detection limits of MUC1 and CEA were 0.13 nM and 2.75 ng mL-1, respectively. More significantly, the present biosensing interface can also be employed for the continuous detection of biomarkers. After the detection of MUC1, the resulted biosensing interface can be continuously used to measured CEA, and the detection limit was obtained as 0.5 ng mL-1, which was obviously lower than that based on the parallel analysis of CEA. Meanwhile, the aptasensor can be regenerated by rehybridization of DNA2 or DNA3-MB after the parallel detection targets, or by introducing MUC1 and CEA and then rehybridizing the MUC1 aptamer and CEA aptamer with linker DNA1 on the gold electrode. Therefore, it can be elucidated that this present strategy provides different protocols (parallel and continuous detection) to meet the requirements of analytical applications. This present method is also cost-effective and operating-simple comparing with the instrument-based methods for the multiplexed detection of markers. And the accuracy, simplicity and reproducibility of this biosensor was excellent. It will give a potential guidance for the multiplexed detection of biomarkers or other small molecules.

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	1. A simple regenerable electrochemical aptasensor for the parallel and continuous detection of biomarkers
	Chunyan Liu,Xi Liu,Yun Qin,Chunyan Deng,Juan Xiang
	Chemistry 13 May 2016
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	Abstract:In this present work, a simple regenerable electrochemical aptasensor for the parallel and continuous detection of protein biomarkers was reported. Mucin 1 protein (MUC1) and cinoembryonic antigen (CEA) were employed as the models of protein biomarkers. The sensing interface was modified by MUC1 aptamer and methylene blue (MB)-modified CEA aptamer via hybridization with a linker DNA. Herein, MB served as a signal transducer, MUC1 and CEA were parallel measured, and the detection limits of MUC1 and CEA were 0.13 nM and 2.75 ng mL-1, respectively. More significantly, the present biosensing interface can also be employed for the continuous detection of biomarkers. After the detection of MUC1, the resulted biosensing interface can be continuously used to measured CEA, and the detection limit was obtained as 0.5 ng mL-1, which was obviously lower than that based on the parallel analysis of CEA. Meanwhile, the aptasensor can be regenerated by rehybridization of DNA2 or DNA3-MB after the parallel detection targets, or by introducing MUC1 and CEA and then rehybridizing the MUC1 aptamer and CEA aptamer with linker DNA1 on the gold electrode. Therefore, it can be elucidated that this present strategy provides different protocols (parallel and continuous detection) to meet the requirements of analytical applications. This present method is also cost-effective and operating-simple comparing with the instrument-based methods for the multiplexed detection of markers. And the accuracy, simplicity and reproducibility of this biosensor was excellent. It will give a potential guidance for the multiplexed detection of biomarkers or other small molecules.
	TO cite this article:Chunyan Liu,Xi Liu,Yun Qin, et al. A simple regenerable electrochemical aptasensor for the parallel and continuous detection of biomarkers[OL].[13 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4689052


	2. The photoelectrochemical sensor based on graphene-supported TiO2 mesocrystal for concanavalin A determination
	Chen Sihong,Zhang Shupei,LI Yilin,Yan Huixiang,Gong Lingshan,Zhang Qingrong,Xu Guifang,Dai Hong
	Chemistry 29 March 2016
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	Abstract: A signal-on photoelectrochemical sensing methodology for concanavalin A monitoring by coupling graphene-supported quasi-octahedral TiO2 mesocrystal with an efficient enzyme-free labeling strategy. Graphene with prominent conductivity enhancing the photocurrent response, NiCo2O4 labeling was proposed here accept the photo-induced electrons from conduction band of TiO2 mesocrystal, leading to the decrease of the photocurrent intensity. A signal-on non-enzymatic photoelectrochemical sensor was constructed based on immobilized concanavalin A competing with isolated concanavalin A of various concentrations for reaction with the NiCo2O4 labeled glucose conjugates, which opened a novel method for concanavalin A determination.
	TO cite this article:Chen Sihong,Zhang Shupei,LI Yilin, et al. The photoelectrochemical sensor based on graphene-supported TiO2 mesocrystal for concanavalin A determination[OL].[29 March 2016] http://en.paper.edu.cn/en_releasepaper/content/4682488

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	3. Dual-responsive competitive strategy based on g-CN /rGO conjugation for tumor marker immunoassay
	Chen Sihong,Zhang Shupei,LI Yilin,Yan Huixiang,Zhang Qingrong,Gong Lingshan,Xu Guifang,Dai Hong
	Chemistry 08 March 2016
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	Abstract: A new dual mode competitive immunosensing platform based on graphite carbon nitride and reduced graphene oxide (g-CN/rGO) conjugate was designed for sensitive determination of α-fetoprotein (AFP) combined photoelectrochemical and electrochemical methods. Horseradish peroxidase labeled antibody was competitively captured onto sensing interface with free antigen in incubated solution. The enzyme-induced electrocatalytical signal amplification was produced by using hydroquinone as reactive substrate and horseradish peroxidase as reactive enzyme in existence of H2O2. This designed dual-responsive immunosensor demonstrated the promising application in developing sensitive and accurate immunosensor for clinical test and disease diagnosis.
	TO cite this article:Chen Sihong,Zhang Shupei,LI Yilin, et al. Dual-responsive competitive strategy based on g-CN /rGO conjugation for tumor marker immunoassay[OL].[ 8 March 2016] http://en.paper.edu.cn/en_releasepaper/content/4680240


	4. A photoelectron signal amplication strategy based on poly(dopamine)-sensitized TiO2 signal crystal for immunoassay
	Chen Sihong,Zhang Shupei,LI Yilin,Gong Lingshan,Yan Huixiang,Zhang Qingrong,Xu Guifang,Dai Hong
	Chemistry 01 March 2016
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	Abstract:A versatile photoelectrochemical immunoassay protocol based on the poly(dopamine)-sensitized titanium dioxide (TiO2) signal crystal with the support of ordered mesoporous carbon. was designed for quantitative monitoring the tumor markers. Poly(dopamine) was introduced to alter the optical properties of TiO2 signal crystal,enzyme-like biomimetic catalyst was exploited as the signal ampli?er to catalyze the hydroquinone. Herein, a competitive-type immunosensor was fabricated through the biomimetic catalyst labeled prostate speci?c antigen competing with the target antigen of different concentrations to react with the specific antibody anchored on the poly(dopamine)-sensitized TiO2 signal crystal, which provided a new photoelectrochemical consideration for tumor markers analysis.
	TO cite this article:Chen Sihong,Zhang Shupei,LI Yilin, et al. A photoelectron signal amplication strategy based on poly(dopamine)-sensitized TiO2 signal crystal for immunoassay[OL].[ 1 March 2016] http://en.paper.edu.cn/en_releasepaper/content/4679543


	5. Ratiometric Electrochemiluminescent Immunosensor based on Mesocrystals and Biomimetic Catalyst for Tumor Marker
	Zhang Qingrong,Xu Guifang,Gong Lingshan,Zhang Shupei,Yan Huixiang,LI Yilin,Chen Sihong,Dai Hong
	Chemistry 01 March 2016
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	Abstract:In this paper, a ratiometric electrochemiluminescent immunosensor based on octahedral anatase mesocrystals (OAM) support for the determination of alpha-fetal protein (AFP) was developed. And OAM with high porosity and large surface area was adopted to as affinity support to immobilize abounding antibodies of AFP (Ab). Luminol and potassium persulfate (K2S2O8) were chosen as promising ECL emitter units, due to the potential-resolved property and maximum emission wavelength-resolved property of them. Furthermore, CS-AgI was found to possess biomimetic catalyst activity, which could catalyze the decomposition of hydrogen peroxide that as the common coreactant both of luminol and K2S2O8 and thus the dual ECL responses was amplified. Upon the basis of the ratio of ECL intensities at two excitation potentials, this proposed ratiometric ECL strategy achieved ultrasensitive determination for the alpha-fetal protein via competitive immunoreaction.
	TO cite this article:Zhang Qingrong,Xu Guifang,Gong Lingshan, et al. Ratiometric Electrochemiluminescent Immunosensor based on Mesocrystals and Biomimetic Catalyst for Tumor Marker[OL].[ 1 March 2016] http://en.paper.edu.cn/en_releasepaper/content/4679439


	6. A innovative electrochemiluminescent immunosensor based on carbon nanodots functionalized with poly(amidoamine) dendrimer for α-fetoprotein detection
	Chen Sihong,Zhang Shupei,LI Yilin,Gong Lingshan,Yan Huixiang,Zhang Qingrong,Xu Guifang,Dai Hong
	Chemistry 29 February 2016
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	Abstract:A sandwich-type electrochemiluminescent biosensor based on carbonaceous based nanomaterials was fabricated for alpha-fetoprotein detection. After the capturing antibody was immobilized onto the modified electrode surface, the detection antibody together with poly(amidoamine) dendrimers functionalized carbon nanodots as the recognizable signal tags was self-assembled onto the electrode by typical sandwich immune reaction. Poly(amidoamine) dendrimers functionalized carbon nanodots make the composite not only an immobilizing platform for carrying numerous detection antibodies, but also an promising promoter for the electrochemiluminescent signal of luminol.This immunosensing strategy for alpha-fetoprotein with the assistance of poly(amidoamine) dendrimers functionalized carbon nanodots provided a promising approach in clinical disease scanning.
	TO cite this article:Chen Sihong,Zhang Shupei,LI Yilin, et al. A innovative electrochemiluminescent immunosensor based on carbon nanodots functionalized with poly(amidoamine) dendrimer for α-fetoprotein detection[OL].[29 February 2016] http://en.paper.edu.cn/en_releasepaper/content/4679177


	7. A novel ultrasensitive electrochemical sensor based on NiCo2O4 nanosuperstrutures for 6-benzylaminopurine determination
	Yan Huixiang,Zhang Qingrong,Gong Lingshan,Xu Guifang,Zhang Shupei,LI Yilin,Chen Sihong,Dai Hong
	Chemistry 31 December 2015
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	Abstract:In this paper, a facile hydrothermal method to synthetise a novel electrode material of NiCo2O4 nanosuperstructures. An ultrasensitive and good stabilized electrochemical sensor based on this NiCo2O4 nanosuperstructures was proposed to determine 6-benzylaminopurine (6-BAP). The electrochemical investigation confirms that NiCo2O4 nanosuperstructures stabilized by polyethyleneimine (PEI) could remarkably boost the electrocatalytic activity for the oxidation response of 6-BAP because of its unique properties of large pore volume, good electrical conductivity and metal catalysis. The fabricated electrochemical sensor showed a wide linear range of 6-BAP detection from 0.1 nM to 10 μM with a low limit of detection 0.1 nM. Therefore, this novel developed sensor based on PEI-NiCo2O4/GCE was also applied to quantitative determination food borne contamination to monitor food safety.
	TO cite this article:Yan Huixiang,Zhang Qingrong,Gong Lingshan, et al. A novel ultrasensitive electrochemical sensor based on NiCo2O4 nanosuperstrutures for 6-benzylaminopurine determination[OL].[31 December 2015] http://en.paper.edu.cn/en_releasepaper/content/4673572


	8. One step preparation of magnetic dual-template molecularly imprinted nanoparticles for specific recognition of two proteins
	ZHAO Siqi,TANG Yuhai,HAO Yi,GAO Ruixia,ZHANG Min
	Chemistry 29 December 2015
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	Abstract:In this paper, a simple one-step imprinted strategy to prepare the core-shell protein imprinted magnetic nanoparticles (DMIPs), based on bovine hemoglobin (BHb) and bovine serum albumin (BSA) as dual templates as well as dopamine (DA) as the monomer and cross-linker, for efficient identification of two high-abundant proteins was described. BHb and BSA were imprinted on the surface of magnetic nanoparticles successively using self-polymerization of DA and surface imprinting technique. The resulting magnetic imprinted nanomaterials were characterized by transmission electron microscope (TEM), X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, and vibrating sample magnetometer (VSM). The results showed that the uniform MIP layer of 8 nm was homogeneously grafted on the surface of magnetic nanoparticles. Moreover, the DMIPs exhibited super-paramagnetic property, resulting in the convenient and highly efficient extraction. A series of adsorption experiments were performed to study the properties of the synthesized DMIPs. The results showed that the DMIPs exhibited the obvious great adsorption performance and satisfactory selectivity for BSA and BHb, which confirmed the effectiveness and feasibility of the dual-template imprinted strategy we proposed. In addition, the as-synthesized nanoparticles could be used for six cycles of adsorption-elution without obvious deterioration, indicating a potential value for simultaneously removal of high-abundant proteins.
	TO cite this article:ZHAO Siqi,TANG Yuhai,HAO Yi, et al. One step preparation of magnetic dual-template molecularly imprinted nanoparticles for specific recognition of two proteins[OL].[29 December 2015] http://en.paper.edu.cn/en_releasepaper/content/4674085


	9. Study on extraction and recovery of almond (Prunus armeniaca L.) protein using aqueous polyethylene glycol as the solvent
	Ge Xianli,Shi Ting,Wang Huan,Zhang Zhiqi
	Chemistry 02 December 2015
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	Abstract:A new method for almond protein extraction and recovery was developed. The extraction efficiencies of different solvents, such as sodium hydroxide, sodium chloride, polyethylene glycol (PEG) 200, PEG 400, and PEG 600 aqueous solutions, were investigated and PEG 200 showed the highest extraction efficiency. The PEG-based microwave-assisted extraction (MAE) parameters were then optimized by using response surface methodology. The optimal extraction parameters were as follows: PEG 200 concentration, 25% (w/w); liquid to solid ratio, 1:22 g/mL; microwave power, 120 W; extraction temperature, 45 C; and extraction time 4 min. At these parameters, the actual average extraction yield was 93.75±3.15%. Subsequently, the almond protein was recovered from the extraction solution containing PEG with an isoelectric point-ethanol synergy precipitation protocol. The combined technique integrated the speed of isoelectric point precipitation with the completeness of alcohol precipitation. The precipitation rate of almond protein was 98.81% with a time of 3-5 min.
	TO cite this article:Ge Xianli,Shi Ting,Wang Huan, et al. Study on extraction and recovery of almond (Prunus armeniaca L.) protein using aqueous polyethylene glycol as the solvent[OL].[ 2 December 2015] http://en.paper.edu.cn/en_releasepaper/content/4667951


	10. Conformation and charge co-modulated ultrasensitive biosensor based on single nanopore channel
	Cai Sheng-Lin,Zheng Yu-Bin,Cao Shuo-Hui,Cai Xiu-Hong,Li Yao-Qun
	Chemistry 28 November 2015
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	Abstract:In this manuscript, we have demonstrated the proof-of-concept of the conformation and surface charge co-modulated ion transport inside a synthetic nanopore engineered by a single stimulus. We integrated an ATP binding aptamer (ABA) into a single glass conical nanopore channel and achieved the simultaneous transitions involving both conformation and charge densities upon the presence of ATP. The synergistic effect of pore size and surface charge has largely enhanced the response efficiency of nanopore. Based on this property, we further developed an ultrasensitive biosensing platform for the detection of ATP with a detection limit down to 0.36 pM (S/N=3).This biomimetic nanodevice exhibited excellent selectivity. This work not only facilitates a better understanding of the ionic transport processes in biological ion channels but also advances their real-world applications in biosensors and actuators.
	TO cite this article:Cai Sheng-Lin,Zheng Yu-Bin,Cao Shuo-Hui, et al. Conformation and charge co-modulated ultrasensitive biosensor based on single nanopore channel[OL].[28 November 2015] http://en.paper.edu.cn/en_releasepaper/content/4666710

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