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1. QCM biosensor for detection of small nucleic acid fragments: sandwich reaction and signal amplification by silver nanoclusters | |||
GUO Jia,JIANG Qifa,Rui-Qin Fang | |||
Chemistry 24 September 2021 | |||
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Abstract:This article presents a quartz crystal microbalance (QCM) biosensor for the determination of nucleic acids by modifying electrodes with cysteine (Cys), polyethylene glycol (PEG), and silver nanoclusters (AgNCs), which represents a new approach with increased specificity and sensitivity of detection. The probe deoxyribonucleic acid immobilized on the surface of the QCM gold electrode hybridizes with a cysteine conjugate to form a specific recognition layer. The unmodified sites are then non-specifically closed by polyethylene glycol. The target gene is subsequently captured by the immobilized probe, resulting in a change in frequency. The addition of silver ions with reducing agents in an ammonia environment to form silver nanoclusters of DNA-AgNCS was used to further extend the sensitivity of the QCM sensor. The results show that it is possible to expand the frequency variation of the QCM sensor by a factor of 63.6 using such a method. A linear relationship between frequency change and DNA concentration was observed in the range of 1 nM to 10 M of target gene concentration. Thus, this biosensor provides a low-cost and sensitive tool for detecting DNA. | |||
TO cite this article:GUO Jia,JIANG Qifa,Rui-Qin Fang. QCM biosensor for detection of small nucleic acid fragments: sandwich reaction and signal amplification by silver nanoclusters[OL].[24 September 2021] http://en.paper.edu.cn/en_releasepaper/content/4755569 |
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