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1. Compacted Sewage Sludge as a Barrier for Tailings: the Bacterial Community Structure Diversity in the Compacted Sludge | |||
ZHANG Qing,ZHANG Huyuan,WANG Jinfang | |||
Environmental Science and Techology 24 July 2015
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Abstract:Compacted sewage sludge barrier for tailings, known as reducing barrier, was innovatively double- functioned liner with its capsulation and elimination to Acid Mine Drainage (AMD) produced by tailings. The research had provided insights regarding the bacterial community structure diversity and dynamics over 75 days in the compacted sewage sludge specimens under 3 different seepage conditions (deionized water (DW), pH 2.1 sulfuric acid water (SA) and the synthetic AMD), by PCR-DGGE technique. The results indicated that at the start point (SP) of seepage, the 41st day (T1) of seepage or at the 75th day (T2) of seepage, under different seepage conditions (DW, SA and AMD), Clostridiales ,Bacillaceae and Carnobacteriaceae dominated in the compacted sewage sludge specimen with different relative abundance ranged from 46.26 % to 10.25 %. Redundancy analysis (RDA) on the influences between environmental factors (pH and heavy metals potential mobility in the compacted sludge specimens) and microbial community structure diversity showed the bacteria such as Carnobacteriaceae, Bacillaceae, Clostridiales Family XI. Incertae Sedis, Clostridiaceae, Lactobacillaceae, Peptoniphilaceae, Ruminococcaceae, Moraxellaceae, Caulobacteraceae, Corynebacteriaceae had positive relation to Zn potential mobility and pH; Carnobacteriaceae, Bacillaceae, Clostridiales Family XI. Incertae Sedis, Clostridiaceae, Micrococcineae, Moraxellaceae, uncertain family bacteria had positive relation with Fe potential mobility; Carnobacteriaceae, Clostridiaceae, Corynebacteriaceae had positive relation to Cd and Ni potential mobility; Ruminococcaceae had positive relation to Cu potential mobility. | |||
TO cite this article:ZHANG Qing,ZHANG Huyuan,WANG Jinfang. Compacted Sewage Sludge as a Barrier for Tailings: the Bacterial Community Structure Diversity in the Compacted Sludge[OL].[24 July 2015] http://en.paper.edu.cn/en_releasepaper/content/4650839 |
2. Indoor heating drives water bacterial growth and community metabolic profile changes in the building tap pipes in winter season | |||
ZHANG Haihan,CHEN Shengnan,BAI Yanru,MA Wenliang,LV Kai | |||
Environmental Science and Techology 11 May 2015 | |||
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Abstract:The growth of bacterial species harbored in the indoor drinking water taps was regulated by external environmental factors, and the people's health and drinking water safe was influenced by water quality of indoor drinking water taps. The indoor temperature increased significantly by indoor heating process. However, the mechanism of effect of indoor heating on bacterial re-growth from indoor drinking water taps was vastly not well understood. In the present work, flow cytometry and BIOLOG techniques were combined to explore the effects of indoor heating process on water bacterial cell concentrations and community metabolic profiles in building tap pipes. The results showed that the temperature of overnight stagnation water in the pipes was 15-17℃, after 10 min flushing of the indoor water taps, the water temperature decreased to 4-6℃. The highest bacterial cell number was observed in before flushing water (overnight stagnation), which is 5-11 times higher than that of flushed water. Meanwhile, the significant higher bacterial community metabolic activity (AWCD590nm) was also found in overnight stagnation water sample. The significant "flushed" and "taps" indicates that the AWCD590nm, and bacterial cell number varied among the taps within the flushed (P<0.01). Heatmap fingerprints and principle component analyses (PCA) revealed that a significant discrimination bacterial cell number and community functional metallic profiles in the overnight stagnation water and flushed water. Serine, Threonine, Glucose-Phosphat, Ketobutyric Acid, Phenylethyl-amine, Glycerol, Putrescine were significantly used by "Before" water samples. Our results suggested that the stagnation water with higher temperature should be discharged before composition. It is therefore that the data from this work can give more information on reasonable utilization of drinking water after stagnation in indoor pipes during the indoor heating process periods. | |||
TO cite this article:ZHANG Haihan,CHEN Shengnan,BAI Yanru, et al. Indoor heating drives water bacterial growth and community metabolic profile changes in the building tap pipes in winter season[OL].[11 May 2015] http://en.paper.edu.cn/en_releasepaper/content/4642162 |
3. Vertical Distribution of Bacterial Community Diversity and Water Quality during the Reservoir Thermal Stratification | |||
ZHANG Haihan,HUANG Tinglin,CHEN Shengnan,SHANG Panlu,BAI Yanru | |||
Environmental Science and Techology 27 April 2015
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Abstract:In this paper, water quality and bacterial community diversity was determined in a seasonal layered JIN PEN drinking water reservoir (Northwest China) using Illumina Miseq high-throughout sequencing targeting the V6 region of the 16S rRNA gene. Water samples were collected from 0.5, 5, 10, 20, 25, 30, 45, 50, 55 and 65 m depth of JIN PEN reservoir during thermal stratification. The results showed that epilimnion, metalimnion and hypolimnion were formed steadily in JIN PEN reservoir. The temperature was decreased steadily from the surface with 23.11℃ to the bottom with 9.17℃. Total nitrogen (TN) from 1.07 to 2.06 mg/L and nitrate nitrogen ranged from 0.8 to 1.84 mg/L. The dissolved oxygen (DO) concentration was decreased sharply below 50 m, and reached to zero in 65 m. The Miseq sequencing revealed a total of 4127 operational taxonomic units (OTUs) with 97% similarity, which were affiliated with 15 phyla including Acidobacteria, Actinobacteria, Armatimonadetes& |