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	1. BnaABF2, a bZIP transcription factor from rapeseed (Brassica napus L.), enhances drought and salt tolerance in transgenic Arabidopsis
	ZHAO Biyan,HU Yufeng,LI Juanjuan,WU Gaobing,YAO Xuan
	Agronomy 13 May 2016
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (4K B)

	Abstract:Abiotic stresses such as drought and salt stresses have a negative affection the growth and productivity of plants. Improvement of stress tolerance through genetic engineering in plants has been reported in intense studies. Transcription factors play vital roles in plant adaptation to stresses by regulating expression of a great deal of target genes. A family of Arabidopsis basic region leucine zipper (bZIP) transcription factors that can recognize and bind to the abscisic acid (ABA)-responsive elements (ABREs) in promoter is named as ABRE binding factors (ABFs)/ABRE binding proteins (AREBs). They play a key role in the regulation of expression of downstream stress-responsive genes in ABA signalling. Genetic transformation of ABF/ABRE transcription factors has been suggested to be an effective approach for engineering stress-tolerant plants. However, whether the ABF/ABRE transcription factors are able to be used for generating stress-tolerant rapeseed plants has not yet been studied.BnaABF2, encoding a basic region leucine zipper (bZIP) transcription factor, was cloned from rapeseed in this study. Subcellular localization and transactivation analyses showed that BnaABF2 was localized to the nucleus with transactivation activity in plant cells. BnaABF2 gene expression was induced by drought and salt stresses and BnaABF2 positively functions in ABA signalling during the vegetative stage. Overexpression of BnaABF2 was found to render drought and salt tolerance to Arabidopsis plants. The resistance of the BnaABF2-expressing transgenic plants to drought and salt stresses is due to reduced water-loss rate and expression of stress-responsive genes such as RD29B, RAB18 and KIN2. The expression of RD29B, RAB18 and KIN2 regulated by BnaABF2 is involved in an ABA-dependent stress signalling.
	TO cite this article:ZHAO Biyan,HU Yufeng,LI Juanjuan, et al. BnaABF2, a bZIP transcription factor from rapeseed (Brassica napus L.), enhances drought and salt tolerance in transgenic Arabidopsis[OL].[13 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4688731


	2. A novel strategy to enhance resistance to Cucumber mosaic virus in tomato by grafting to transgenic rootstocks
	BAI Miao,CHEN Wenting,XIE Bingyan,YANG Guoshun
	Agronomy 11 May 2016
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (4K B)

	Abstract:Cucumber mosaic virus (CMV) can infect a wide range of host species. For the lacking of CMV-resistant varieties of tomato, RNA interference can be used as a fast and effective method for the generation of transgenic resistant varieties. In this current study, five intron-spliced hairpin RNA (ihpRNA) plant expression vectors aim at five genes of CMV has been constructed. Transgenic tomatoes were obtained by Agrobacterium mediated transformation with expression vectors. Highly resistant generations of transgenic plants were employed as rootstocks and grafted onto non-transgenic tomatoes that resulted in the successful transfer of resistance to the scions. Using a novel method of plant cuttings for rootstock propagation, we obtained large quantities of disease-resistant material. Further, this method produces scions that can remain undetectable for transgenic resistance marker genes that may provide novel approaches to evade collective concerns about genetically-modified organism (GMO) biosafety.
	TO cite this article:BAI Miao,CHEN Wenting,XIE Bingyan, et al. A novel strategy to enhance resistance to Cucumber mosaic virus in tomato by grafting to transgenic rootstocks[OL].[11 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4687477


	3. Characterization and function of Tomato yellow leaf curl virus-derived small RNAs generated in tolerant and susceptible tomato varieties
	BAI Miao,YANG Guoshun,LIN Runmao,CHEN Wenting,LING Jian,Mao Zhenchuan,XIE Bingyan
	Agronomy 11 May 2016
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (4K B)

	Abstract:Virus-tolerant plant, which allows the accumulation of virus and then generate virus-derived small RNAs (vsRNAs), valuable materials to reveal the antiviral efficiency of vsRNAs. Here, a comparison of vsRNAs in Tomato yellow leaf curl virus tolerant and in susceptible tomato varieties showed the consistent trend of vsRNAs' distribution on virus genome, which is presented as an obvious characteristic. However, the expression level of vsRNA in tolerant variety is less than that in susceptible variety. Slicing targets of vsRNA-mediated viral transcripts were investigated using parallel analysis of RNA ends, and geminivirus DNA methylation was determined by bisulfite sequencing, which uncovered that not all vsRNAs participated in viral mRNA degradation and DNA methylation. Additionally, by comparing with the expression pattern of vsRNAs, viral DNA and mRNA, we proposed the quantity of vsRNAs is corresponding to the expression level of viral mRNA, while the virus-suppression of vsRNAs is not high-efficient.
	TO cite this article:BAI Miao,YANG Guoshun,LIN Runmao, et al. Characterization and function of Tomato yellow leaf curl virus-derived small RNAs generated in tolerant and susceptible tomato varieties[OL].[11 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4687558


	4. Molecular cloning and functional characterization of apple MdPIF1
	Li Yuanyuan,Qiao Yu,Mao Ke,Hao Yujin
	Agronomy 05 May 2016

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