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	1. The structure and functions of SARS-CoV-2 nucleocapsid protein
	SONG Aling,GE Xingyi,QIU Ye
	Biology 18 April 2023
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	Abstract:Severe Acute respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the third highly pathogenic human coronavirus after SARS-CoV and MERS-CoV. SARS-CoV-2 can not only cause flu-like symptoms such as cough, fever, fatigue and sore limbs, but also lead to complications such as pulmonary fibrosis, acute kidney injury and neurodegenerative diseases in partial patients. As the most abundant protein in SARS-CoV-2, N protein participates in the packaging and transcription of viral RNA, and moreover plays important roles in affecting host cell homeostasis. Previous researches demostrated that N protein triggers activation of the Smad3 signaling pathway through the p21‐ dependent G1 cell cycle arrest mechanism which leads to cell death, N protein also inhibits GSDMD-mediated pyroptosis to inhibit the release of IL-1β. N protein regulating host cell antiviral immune response through NF-κB, JAK-STAT and other signaling pathways has been revealed. In addition, studies have proven that N protein is associated with many complications, such as Parkinson\'s disease, pulmonary fibrosis and so on. In this review, we describe the known structure of SARS-CoV-2 N protein, its role in packaging viral RNA and effects on host cell homeostasis.
	TO cite this article:SONG Aling,GE Xingyi,QIU Ye. The structure and functions of SARS-CoV-2 nucleocapsid protein[OL].[18 April 2023] http://en.paper.edu.cn/en_releasepaper/content/4760370


	2. Study on the renaturation process of epidermal growth factor-like protein and its therapeutic effect on neuroinflammation
	Yang Jingru,He Huahong,He Huafeng,Wang Huaqian
	Biology 25 March 2023
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	Abstract:Epidermal growth factor (EGF) plays an important role in improving the inflammatory response. In this study, cholera toxin A2 subunit, cell-penetrating peptide trans-transcription activator (TAT) and EGF were fused and recombinant protein EGF-CTA2-TAT were expressed by Escherichia coli. Two methods of renaturation, affinity chromatography and dialysis, were selected to renature the protein. The renaturation efficiency of these two methods were compared. Activities of recombinant protein on promoting BALB/c 3T3 cells proliferation in vitro were also studied. After that, mice model of neuroinflammation was established by using the Lipopolysaccharide (LPS) intraperitoneal injection. The purified and renatured recombinant EGF-like protein was administered intranasally for neuroinflammation treatment. The Morris water maze (MWM) test was used to evaluate the therapeutic effects. Our results showed that recombinant EGF-like protein had a therapeutic effect on LPS-induced neuroinflammation in mice, and could remarkably improve the learning and memory ability of mice. The recombinant EGF-like protein renatured and purified by affinity chromatography in one step shows the advantages of simple process, high yield, excellent activity and low cost, providing a reference for mass production of such active proteins.
	TO cite this article:Yang Jingru,He Huahong,He Huafeng, et al. Study on the renaturation process of epidermal growth factor-like protein and its therapeutic effect on neuroinflammation[OL].[25 March 2023] http://en.paper.edu.cn/en_releasepaper/content/4759631


	3. Screening of strains from reconstituted tobacco sheet making high-yield enzymes and aroma
	Yang Chunxue,Deng Zhang,Liao xiang ru,Guan Zhengbing
	Biology 01 March 2023
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	Abstract:Reconstituted tobacco sheet (RTS) is critical to reduce the harm of cigarettes and considered as a comprehensive utilization approach for tobacco wastes in the tobacco industries. The aim of this study was to screen out bacteria from RTS materials capable of producing a high total amount of enzymes and aroma. A total of 21 strains with unique morphologies were obtained. Based on the results of enzymes and aroma producing capacities of the strains, one strain was screened. This strain, which was named B-NS-7, was capable of producing a total amount of 696.3 U/mL protease activity, 238.4 U/mL amylase activity, 64.3 U/mL pectinase activity and 100.0 μg/mL aroma. Combined with the results of the 16S rDNA sequencing, physiological and biochemical characteristics B-NS-7 was identified as Bacillus subtilis. Through a series of single factor experiences, the results showed that under the conditions of inoculation concentration of 1%, fermentation temperature of 37 C and time of 24 h, the highest aroma content of concentrated solutions were 116.3 μg/mL. Neophytadiene was responsible for the majority of the fragrance enhancement, and B-NS-7 was identified as an aldehydic and alkene-flavoured strain. The sensory quality of tobacco concentration solutions was superior under optimal conditions. This strain has been deposited at the China General Microbiological Culture Collection Center (CGMCC No. 23716). By way of this study, B. subtilis B-NS-7 was intended to be used in the manufacturing of RTS through microbial fermentation to improve the quality and providing suggestions for the microbial development of the RTS industry.
	TO cite this article:Yang Chunxue,Deng Zhang,Liao xiang ru, et al. Screening of strains from reconstituted tobacco sheet making high-yield enzymes and aroma[OL].[ 1 March 2023] http://en.paper.edu.cn/en_releasepaper/content/4759360


	4. Personalized prognostic signature for lung cancer based on 15 transcription-related gene pairs
	Liao Zili,Ren Zhihao,Zhang Boxiang,Zhu Ruiyu
	Biology 15 February 2023
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