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1. Effects of the cultured Cordyceps exopolysaccharide (EPS) on some parameters of mouse immune function in vivo and in vitro1 | |||
Weiyun Zhang,Jinyu Yang,Jiaping Chen,Jing Li,Yi Zheng | |||
Basic Medicine 14 December 2005 | |||
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Abstract:The Cordyceps fungi (CSG1) were cultured and the exopolysaccharide (EPS) was prepared from the culture supernatant. In vivo experiment, ICR mice were treated with three different doses of EPS for seven days. In vitro study, macrophage, spleen and thymus lymphocytes were incubated in the presence of five different concentrations of EPS. The phagocytosis of peritoneal macrophage was determined by neutral red uptake test and the proliferation abilities of spleen and thymus lymphocyte were studied with MTT method. The results of in vivo study illustrated that the macrophage neutral red uptake ability of mice was significantly enhanced by three different doses of EPS (p<0.001) and spleen lymphocyte’s proliferation was elevated at the dose of 60 mg/kg (p<0.01). However, thymus lymphocyte proliferation did not show significant variation compared with the control mice after seven day’s EPS treatment. In vitro study, EPS not only significantly elevated the phagocytosis of peritoneal macrophage in the concentration of 50 and 100 mg/L (p<0.05), but also significantly increased the proliferation abilities of spleen and thymus lymphocytes in 100 mg/L (p<0.001 and p<0.05, respectively). These results suggest that EPS has an immunomodulatory activity on mouse macrophages and lymphocytes, and this ability might be useful for application to assist treating some diseases such as cancers. | |||
TO cite this article:Weiyun Zhang,Jinyu Yang,Jiaping Chen, et al. Effects of the cultured Cordyceps exopolysaccharide (EPS) on some parameters of mouse immune function in vivo and in vitro1 [OL].[14 December 2005] http://en.paper.edu.cn/en_releasepaper/content/4362 |
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