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There are 13 papers published in subject: > since this site started. |
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1. The Circadian Clock in Immune Regulation | |||
JIANG Yunxin,FU Yadong,ZHAO Qihao,MA Dongcheng,LIU Chao,ZHONG Yingbin | |||
Biology 21 December 2023 | |||
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Abstract:The circadian clock is an intrinsic timekeeping mechanism enables organisms to adapt to day-night environmental changes. It regulates physiological and behavior activities in organisms in an approximate 24-hour cycle. The molecular mechanism of the circadian clock is composed of a transcriptional-translational feedback loop. Ample evidence reveals that the immune system, as an important defense mechanism of the body, is under circadian regulation. We summarize recent studies of the influence of the circadian clock on pathogen infection, the immune cells activities and immune disease. The complexs relationship between body clock, immune system, and other systems are also discussed. Finnally, we provide suggestions on the direction and importance of further research on the circadian clock and the immune system. (10 Points, Times New Roman) | |||
TO cite this article:JIANG Yunxin,FU Yadong,ZHAO Qihao, et al. The Circadian Clock in Immune Regulation[OL].[21 December 2023] http://en.paper.edu.cn/en_releasepaper/content/4761742 |
2. Ghrelin reduces A-type potassium channels in nigral dopaminergic neurons via PKCδ pathway | |||
SHI Limin,LI Chen,JIANG Hong | |||
Biology 14 May 2016 | |||
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Abstract:Ghrelin is an endogenous ligand of the growth hormone secretagogue receptor (GHS-R). Through affecting the electrical properties of neurons in central nervous system, ghrelin exerts multiple effects, including appetite stimulation, energy balance modulation and neuroprotection. Our previous study showed that ghrelin increased the firing frequency of nigral dopaminergic neurons via inhibition of Kv7/KCNQ/M channels through activation of GHS-R/PLC/PKC pathway. However, whether the other potassium channels are also involved in the ghrelin-induced excitability of dopaminergic neurons still remain unclear. In this study, we focus on two voltage-gated potassium channels, the delayed rectifier potassium channels (IK) and A-type potassium channels (IA), which have a wide expression on dopaminergic neurons. Using whole-cell patch clamp recordings in vitro, our results indicated that ghrelin could reversibly inhibit IK and IA. The inhibition of IA showed an dose-dependent tendency, as the amplitude of IA decreased by 33.43% ± 3.78% and 52.92%±6.56% after 10 nM and 100 nM ghrelin treatment, respectively. These inhibitory effects of ghrelin were mediated by PKCδ system. The broad spectrum voltage-gated potassium channels blocker TEA, IA specific blocker 4-AP, and PKCδ specific blocker Rottlerin, occluded the excitatory effects of ghrelin. These results demonstrated that inhibition of IK and IA may contribute to the ghrelin-induced excitation of dopaminergic neurons. | |||
TO cite this article:SHI Limin,LI Chen,JIANG Hong. Ghrelin reduces A-type potassium channels in nigral dopaminergic neurons via PKCδ pathway[OL].[14 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4690524 |
3. Wnt-5a Prevents Amyloid β Protein-Induced Deficits in the Hippocampal Synaptic Plasticity in Rats in Vivo | |||
ZHANG Guili,ZHANG Jun,LI Shaofeng,LEI Liu,XIE Yanhong,DENG Fang,FENG Jiachun,QI Jinshun | |||
Biology 06 March 2014 | |||
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Abstract:Although the neurotoxicity of amyloid β (Aβ) protein in Alzheimer's disease (AD) has been reported widely, the exact molecular mechanism underlying the synaptic dysfunction of Aβ is not yet completely understood. Growing evidence indicates that wingless-type (Wnt) signaling plays an important role in neuronal development, synapse formation and synaptic plasticity. In the present study, we investigated the neuroprotective action of Wnt-5a against the synaptic damage induced by Aβ25-35 by using an in vivo electrophysiological technique. We found that intracerebroventricular (i.c.v) injection of Aβ25-35 alone did not affect the baseline field excitatory postsynaptic potentials (fEPSPs) and the paired-pulse facilitation (PPF) in the hippocampal CA1 region of rats, but significantly suppressed high frequency stimulation (HFS) induced long-term potentiation (LTP); pretreatment with Wnt-5a prevented the Aβ25-35-induced suppression of hippocampal LTP in a dose-dependent manner; soluble Frizzled-related protein (sFRP), a specific Wnt antagonist, effectively attenuated the protective effects of Wnt-5a on the hippocampal LTP. These results clarify a probable pathogenesis by which Aβ impairs synaptic plasticity and suggest that Wnt-5a could be used as one of the putative candidates for the therapeutic intervention of AD. | |||
TO cite this article:ZHANG Guili,ZHANG Jun,LI Shaofeng, et al. Wnt-5a Prevents Amyloid β Protein-Induced Deficits in the Hippocampal Synaptic Plasticity in Rats in Vivo[OL].[ 6 March 2014] http://en.paper.edu.cn/en_releasepaper/content/4588975 |
4. Apolipoprotein E4 Impairs in vivo Hippocampal Long-Term Synaptic Plasticity by Reducing the Phosphorylation of CaMKIIα and CREB | |||
QIAO Feng,GAO Xiuping,YUAN Li,CAI Hongyan,QI Jinshun | |||
Biology 06 March 2014 | |||
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Abstract:Inheritance of the apolipoprotein E genotype ε4 (APOE4) is a powerful risk factor for most cases of late-onset Alzheimer's disease (AD). However, the effects of apoE4 on the long-term synaptic plasticity and its underlying mechanism have not clearly investigated. In the present study, we examined the effects of apoE4 on hippocampal late-phase long-term potentiation (L-LTP) and investigated its probable molecular mechanisms by using in vivo field potential recording, immunohistochemistry and western blotting. The results showed that: (1) intra-hippocampal injection of 0.2 μg apoE4, but not apoE2, before high frequency stimulations (HFSs) attenuated the induction of hippocampal L-LTP in the CA1 region, while injection of the same concentration of apoE4 after HFSs, even at a higher concentration (2 μg), did not affect the long term synaptic plasticity; (2) apoE4 injection did not affect the paired pulse facilitation (PPF) in the hippocampal CA1 region; (3) apoE4 injection before, not after, HFSs significantly decreased the levels of phospharylated Ca2+/calmodulin-dependent protein kinase II (p-CaMKII) and phospharylated cAMP response element-binding protein (p-CREB) in the hippocampus. These results demonstrated for the first time that apoE4 could impair hippocampal L-LTP by reducing p-CaMKIIα and p-CREB, suggesting that the apoE4-induced suppression of hippocampal long-term synaptic plasticity may contribute to the cognitive impairments in genetic AD; and both CaMKII and CREB are important intracellular targets of the neurotoxic apoE4. | |||
TO cite this article:QIAO Feng,GAO Xiuping,YUAN Li, et al. Apolipoprotein E4 Impairs in vivo Hippocampal Long-Term Synaptic Plasticity by Reducing the Phosphorylation of CaMKIIα and CREB[OL].[ 6 March 2014] http://en.paper.edu.cn/en_releasepaper/content/4588972 |
5. Unexpected Improvements of Spatial Learning and Memory Abilities in Chronic Rotenone Intoxicated Mice | |||
JIA Fengju,SONG Ning,ZHAO Chenyang,XIE Junxia,JIANG Hong | |||
Biology 20 February 2014 | |||
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Abstract:The liposoluble insecticide rotenone is commonly used as a mitochondrial complex I inhibitor to replicate Parkinson's disease (PD) pathological features. However, there was no assessment of the spatial learning and memory abilities in chronic rotenone-induced PD models. In the present study, by rotarod test and Thioflavine T staining, we first noted the impairment of motor coordination in rotenone-treated group for 3 months, as well as alpha-synuclein inclusions in the nigral dopaminergic neurons in C57BL/6 mice with intragastrical delivery of rotenone (5 mg/Kg) for 3 months rather than 1 month. We then evaluated spatial learning and memory abilities by Morris water maze task in this model. The results showed escape latency reduced in rotenone-intoxicated mice for 3 months, indicating an improvement of learning ability. However, it was delayed slightly but not significantly in rotenone-intoxicated mice for 1 month. Similarly, we demonstrated that spatial memory ability was enhanced in 3-month-treatment group, but impaired in 1-month-treatment group. There were no proliferating cell nuclear antigen and doublecortin positive cells in the hippocampus by double immunofluorescent staining, indicating the absence of hippocampal neurogenesis in rotenone-intoxicated mice. These results suggest that spatial learning and memory abilities are disturbed in chronic rotenone-intoxicated PD model. | |||
TO cite this article:JIA Fengju,SONG Ning,ZHAO Chenyang, et al. Unexpected Improvements of Spatial Learning and Memory Abilities in Chronic Rotenone Intoxicated Mice[OL].[20 February 2014] http://en.paper.edu.cn/en_releasepaper/content/4586368 |
6. Extravagant distribution of Izumo1 protein on entire rat sperm surface | |||
Wang Zheng,Yan Yu,Ma Yuanyuan,Chao Lumeng,Xing Wanjin | |||
Biology 10 January 2014 | |||
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Abstract:Testis-specific protein Izumo1 is one of the sperm-specific proteins that are involved in sperm-egg fusion, and is essential to mouse sperm-egg fusion. But aside from the limited reports in mouse, its expression, regulation and function had hardly been investigated in a broad range of species. To portray the distribution pattern of rat Izumo1 in testis and sperm, we prepared and purified an anti-rat Izumo1 polyclonal antibody by immunizing Kunming mice with recombinant Trx-Izumo1 fusion protein. Immunohistochemical analysis on the localization of Izumo1 in rat testis and sperm using this antibody revealed that Izumo1 is expressed in cells of nearly all stages of developing sperm cells in the stratified epithelium of the seminiferous tubules from the basement membrane toward the lumen of the tubule. It is mainly localized at the anterior pole of the acrosome of sperm head, however, spreads nearly over the whole membrane including the tail. These results provided data about the spatial and temporal expression pattern of this key gamete fusion protein in the model animal rat. | |||
TO cite this article:Wang Zheng,Yan Yu,Ma Yuanyuan, et al. Extravagant distribution of Izumo1 protein on entire rat sperm surface[OL].[10 January 2014] http://en.paper.edu.cn/en_releasepaper/content/4581817 |
7. Changes of proteins induced by anticoagulants can be more sensitively detected in urine than in plasma | |||
Li Menglin,Zhao Mindi,Gao Youhe | |||
Biology 08 November 2013 | |||
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Abstract:Biomarker studies commonly monitor the composition of plasma, which is under strict homeostatic control, while urine is not. Urine is a partial filtrate of blood; however, its composition reflects systemic physiology. Here we show that changes in the coagulation status of blood can be more sensitively detected in urine than in plasma. Plasma and urine protein composition was analysed by LC-MS/MS and Western blot in six SD female rats before and after treatment with heparin or argatroban. LC-MS/MS and Western blot analyses demonstrated drug-induced increases in transferrin and hemopexin levels in urine but not in plasma. Our data indicates that urine may serve as a source for more sensitive detection of protein biomarkers than plasma. | |||
TO cite this article:Li Menglin,Zhao Mindi,Gao Youhe. Changes of proteins induced by anticoagulants can be more sensitively detected in urine than in plasma[J]. |
8. Protective effects of obestatin are mediated by proliferative rather than anti-apoptotic properties in MES23.5 dopaminergic cells | |||
SHEN Xiaoli,JIA Fengju,SONG Ning,XIE Junxia,JIANG Hong | |||
Biology 28 October 2013 | |||
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Abstract:In this paper Objective Obestatin is an endogenous peptide sharing the same peptide precursor with ghrelin. We aimed to investigate whether and how obestatin could exert neuroprotective effects on MES23.5 dopaminergic cells against 1-methyl-4-phenylpyridinium (MPP+)-induced neurotoxicity. Methods MES23.5 cells were pretreated with obestatin (10-13 ~ 10-6 mol/L) for 20 min prior to 200 μmol/L MPP+ incubation for 12 or 24 hrs, or treated with different doses of obestatin (10-13 ~ 10-7 mol/L) for 0, 6, 12, 24 hrs, respectively. Methyl thiazolyl tetrazolium (MTT) assay was used to measure cell viability. Flow cytometry was used to measure the caspase-3 activity and the mitochondrial transmembrane potential. Proliferating cell nuclear antigen (PCNA) protein levels were determined by western blotting. Results Obestatin (10-13 ~ 10-7 mol/L) pretreatment could block or even reverse MPP+-induced reduction of cell viability in MES23.5 cells. Solely obestatin treatment increased cell viability. However, it had no effects on MPP+-induced mitochondrial transmembrane potential collapse and caspase-3 activation. Elevated PCNA levels were observed with 10-7, 10-9, 10-11 and 10-13 mol/L obestatin treatment for 12 hrs. Conclusion The protective effects of obestatin against MPP+ in MES23.5 cells were due to its proliferative rather than anti-apoptotic effects. | |||
TO cite this article:SHEN Xiaoli,JIA Fengju,SONG Ning, et al. Protective effects of obestatin are mediated by proliferative rather than anti-apoptotic properties in MES23.5 dopaminergic cells[OL].[28 October 2013] http://en.paper.edu.cn/en_releasepaper/content/4566453 |
9. A novel approach to predict subjective pain perception from single-trial laser-evoked potentials | |||
Xiao Ping,Huang Gan,Zhang Zhiguo,Hu Li | |||
Biology 22 May 2013 | |||
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Abstract:Pain is a subjective first-person experience, and self-report is the gold standard for pain assessment in clinical practice. However, self-report of pain is not available in some vulnerable populations (e.g., patients with disorders of consciousness), which leads to an inadequate or suboptimal treatment of pain. Therefore, the availability of a physiology-based and objective assessment of pain that complements the self-report would be of great importance in various applications. Here, we aimed to develop a novel and practice-oriented approach to predict pain perception from single-trial laser-evoked potentials (LEPs). We applied a novel single-trial analysis approach that combined common spatial pattern and multiple linear regression to automatically and reliably estimate single-trial LEP features. Further, we adopted a Na?ve Bayes classifier to discretely predict low and high pain and a multiple linear prediction model to continuously predict the intensity of pain perception from single-trial LEP features, at both within- and cross-individual levels. Our results showed that the proposed approach provided a binary prediction of pain (classification of low pain and high pain) with an accuracy of 86.3±8.4 % (within-individual) and 80.3±8.5 % (cross-individual), and a continuous prediction of pain (regression on a continuous scale from 0 to 10) with a mean absolute error of 1.031 ± 0.136 (within-individual) and 1.821 ± 0.202 (cross-individual). Thus, the proposed approach may help establish a fast and reliable tool for automated prediction of pain, which could be potentially adopted in various basic and clinical applications. | |||
TO cite this article:Xiao Ping,Huang Gan,Zhang Zhiguo, et al. A novel approach to predict subjective pain perception from single-trial laser-evoked potentials[J]. |
10. Distinct features of auditory steady-state responses as compared to transient event-related potentials | |||
ZHANG Li,PENG Weiwei,ZHANG Zhiguo,HU Li | |||
Biology 20 May 2013 | |||
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Abstract:Transient event-related potentials (ERPs) and steady-state responses (SSRs) have been popularly employed to investigate the function of the human brain, but their relationship still remains a matter of debate. Some researchers believed that SSRs could be explained by the linear summation of successive transient ERPs (superposition hypothesis), while others believed that SSRs were result of the entrainment of a neural rhythm driven by the periodic repetition of a sensory stimulus (oscillatory entrainment hypothesis). In the present study, taking auditory modality as an example, we aimed to clarify the distinct features of SSRs, evoked by the 40-Hz and 60-Hz periodic auditory stimulation, as compared to transient ERPs, evoked by a single click. We observed that (1) SSRs were mainly generated by phase synchronization, while late latency responses (LLRs) in transient ERPs were mainly generated by power enhancement; (2) scalp topographies of LLRs in transient ERPs were markedly different from those of SSRs; (3) the powers of both 40-Hz and 60-Hz SSRs were significantly correlated, while they were not significantly correlated with the N1 power in transient ERPs; (4) whereas SSRs were dominantly modulated by stimulus intensity, middle latency responses (MLRs) were not significantly modulated by both stimulus intensity and subjective loudness judgment, and LLRs were significantly modulated by subjective loudness judgment even within the same stimulus intensity. All these findings indicated that high-frequency SSRs were different from both MLRs and LLRs in transient ERPs, thus supporting the possibility of oscillatory entrainment hypothesis to the generation of SSRs. Therefore, SSRs could be used to explore distinct neural responses as compared to transient ERPs, and help us reveal novel and reliable neural mechanisms of the human brain. | |||
TO cite this article:ZHANG Li,PENG Weiwei,ZHANG Zhiguo, et al. Distinct features of auditory steady-state responses as compared to transient event-related potentials[J]. |
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