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1. Isolate, puri?cation and antioxidant activities of polysaccharides from Dioscoreae bulbiferae | |||
CUI Hongxia,SU Yan | |||
Biology 29 December 2017 | |||
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Abstract:A water-soluble polysaccharide from Dioscorea bulbifera (Dioscoreaceae) rhizome was isolated by extraction with water extract and alcohol precipitate and further purified by DEAE cellulose-52 chromatography to yield DBP-1 and DBP-2. The total yield rate of the polysaccharides was 4.3 %. Molecular weights were determined by high performance gel permeation chromatography (HPGPC). The molecular weights of DBP-1 and DBP-2 were 122435Da and 5180Da, respectively. The antioxidant activity of DBP-1 and DBP-2 were evaluated with the scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals in vitro, and the results indicated that DBP-1 and DBP-2 had good antioxidant activity. These results suggested that the polysaccharides (DBP-1 and DBP-2) obtained from the Dioscorea bulbifera might be suitable for use as functional foods and potential therapeutic agents for human aging. | |||
TO cite this article:CUI Hongxia,SU Yan. Isolate, puri?cation and antioxidant activities of polysaccharides from Dioscoreae bulbiferae[OL].[29 December 2017] http://en.paper.edu.cn/en_releasepaper/content/4743023 |
2. Identification of hybrids in Potamogeton:incongruence between plastid and ITS regions solved by a novel barcoding marker PHYB | |||
Tao Yang,Tian-lei Zhang,Ming-fang Du,Feng-qin Tian,Xing Liu,Youhao Guo | |||
Biology 14 June 2016 | |||
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Abstract:Potamogeton is one of the most difficult groups to clarify in aquatic plants, which have an extensive range of interspecific morphological and ecological diversity. Internal transcribed spacer (ITS) of nuclear ribosomal DNA is prevalent for phylogenetic analysis in plants. However, most researches have demonstrated that ITS regions show a high percentage of homoplasy in phylogenetic data sets. In this study, eighteen materials were collected in the genus Potamogeton from China and an incongruence was shown between the rbcL and ITS phylogenies. To solve this discrepancy, we sequenced a novel barcode PHYB gene to improve resolution and accuracy of the phylogenetic relationships. The PHYB phylogenetic analysis successfully resolved the incongruence between the rbcL and ITS phylogenies. In addition, six hybrids were confirmed using the PHYB marker, including P. compressus × P. pusillus, P. octandrus × P. oxyphyllus, P. gramineus × P. lucens, P. distinctus × P. natans, P. distinctus × P. wrightii, and S. pectinata × S. amblyophylla. Whereas, only one hybrid was identified (P. compressus × P. pusillus) for the ITS phylogeny, indicating that ITS homoplasy is present in Potamogeton and ITS regions completely homogenized towards to one parental type. Thus, ITS regions maybe have limited utility in phylogenetic relationships for Potamogeton. It is recommended that a three-locus combination of chloroplast DNA, ITS and PHYB is potential to effectively reveal more robust phylogenetic relationships and species identification. | |||
TO cite this article:Tao Yang,Tian-lei Zhang,Ming-fang Du, et al. Identification of hybrids in Potamogeton:incongruence between plastid and ITS regions solved by a novel barcoding marker PHYB[OL].[14 June 2016] http://en.paper.edu.cn/en_releasepaper/content/4697545 |
3. Cloning and expression analysis of LeMYC gene in Lithospermum Erythrorhizon | |||
Hu Zhao,Jinliang Qi,Yonghua Yang | |||
Biology 17 May 2016 | |||
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Abstract:LeMYC is a novel member of the basic helix-loop-helix (bHLH) family that was cloned from Lithospermum erythrorhizon by using rapid amplification of cDNA ends method. Bioinformatics analyses showed that the predicted LeMYC protein contained a potential bHLH domain and was highly homologous to AtMYC2 from Arabidopsis thaliana. The promoter of LeMYC was further cloned to analyze its expression pattern, and sequence analysis revealed a number of potential regulatory motifs related to tissue-specific gene expression and abiotic and biotic stress responses. Real-time PCR results suggested that LeMYC was significantly induced transiently during the early stage when L. erythrorhizon cells were transferred from a B5 to an M9 medium to form shikonin. Exogenous methyl jasmonate (MeJA), an effective inducer of shikonin accumulation, also remarkably induced the rapid LeMYC expression. By contrast, ibuprofen (IBU), an inhibitor of jasmonate biosynthesis, significantly inhibited LeMYC expression. Tissue-specific expression analysis showed that LeMYC mRNA predominantly accumulated in the roots where shikonin was biosynthesized. These results indicated that LeMYC gene could be a novel member of the bHLH gene family and may have an important function in shikonin formation. | |||
TO cite this article:Hu Zhao,Jinliang Qi,Yonghua Yang. Cloning and expression analysis of LeMYC gene in Lithospermum Erythrorhizon[OL].[17 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4690111 |
4. TEMPRANILLO1 transcription factor AtTEM1 negatively regulates drought and mannitol tolerance in Arabidopsis | |||
LUO Guangyu,LIU Ailing,ZHOU Xiaoyun,ZHANG Xianwen,CHEN Xinbo | |||
Biology 11 May 2016 | |||
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Abstract:Arabidopsis TEMPRANILLO 1 (AtTEM1) is a member of the RAV transcription factor subfamily with plant-specific AP2 and B3 domains. Which are known to negatively control plant growth and delay flowering time. In this study, we have demonstrated that Arabidopsis AtTEM1 is localized in the nucleus, consistent with its putative role as a transcription factor. Expression of AtTEM1 was strongly induced by drought and mannitol treatments. Drought tolerances were significantly decreased in 35S::AtTEM1 transgenic Arabidopsis, but were enhanced in the AtTEM1 mutant plants. Under drought and mannitol stress, the 35S::AtTEM1 transgenic Arabidopsis exhibited increased relative electrical conductivity (REC) and content of malondialdehyde (MDA) and decreased proline content compared with the wild type, while the lower REC and MDA content and increased proline content were found in the AtTEM1 mutant plants. These results suggest that AtTEM1 functions as a negative regulator in response to drought and mannitol stresses in Arabidopsis. AtTEM1 also acts as a negative regulator for flowering time response. The 35S::AtTEM1 plants bolted later than the wildtype and produced extra rosette leaves before flowering, however the AtTEM1 mutant plants bolted earlier but produced almost the same rosette leaves before flowering. | |||
TO cite this article:LUO Guangyu,LIU Ailing,ZHOU Xiaoyun, et al. TEMPRANILLO1 transcription factor AtTEM1 negatively regulates drought and mannitol tolerance in Arabidopsis[OL].[11 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4687336 |
5. Cell walls are likely associated with polyamine-induced NO generation in soybean cotyledon node callus | |||
Gao Fenming,Xu Gangming,Zeng Junjie,Su Guoxing | |||
Biology 25 December 2015 | |||
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Abstract:Our previous studies have showed that diamine oxidase (CuAO) from soybean cotyledon callus may participate in polyamine-induced NO generation, but still need substantial evidence. CuAO is mainly located in cell walls. In this paper, by protoplast culture through removing cell walls, whether cell walls were associated with the process of polyamine-induced NO generation was studied. Firstly, the susoension cells from soybean cotyledon node callus were used as material, It was found that all three kinds of exogenous polyamines (Put, Spd and Spm) could effectively induce NO fluorescence, and Put was most effective. It was also found that this NO fluorescence was significantly suppressed by addition of cPTIO (a NO specific scavenger), or aminoguanidine (AG, a specific inhibitor of CuAO), indicating its NO nature and its possible relation with CuAO. The test of NO relative fluorescence units (RFU) showed that their changes were consistent with the changes of CuAO activities caused by the above treatmnts. Secondly, through enzymolysis of suspension cells to obtain energetic protoplasts with high density, equally treated with three kinds of exogenous polyamine, polyamines-induced NO release was observed to decrease significantly in the protoplasts, compared with the control suspension cells, concomitantly its NO RFU value greatly dropped. In order to further verify the relationship between polyamine-induced NO release and cell walls, the regeneration experiment of protoplast cell walls was carried out. Using fluorescent brightening agents (FBA) to indicate the regeneration process of protoplast cell walls, after treatments with three kinds of exogenous polyamines, NO fluorescence first appeared in the edge of the cells, and along with the degree of cell wall integrity, NO fluorescence intensity gradually increased, and reached maximum after 7 d cells. It was interesting to note that the CuAO activity had been gradually recovered with the regeneration of the protoplast cell walls. These data suggest that cell walls are associated with the polyamine-induced NO production, which may be related to the cell wall located-CuAO activities. | |||
TO cite this article:Gao Fenming,Xu Gangming,Zeng Junjie, et al. Cell walls are likely associated with polyamine-induced NO generation in soybean cotyledon node callus[OL].[25 December 2015] http://en.paper.edu.cn/en_releasepaper/content/4673519 |
6. Overexpression of GsCBRLK gene enhanced salt tolerance of soybean plants | |||
LI Sheng,JI Wei | |||
Biology 04 December 2015 | |||
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Abstract:Salinity is one of the serious environmental constraints limiting crop plants growth and yield. We have previously reported that GsCBRLK functions as a positive regulator of plant tolerance to salt stress. In order to investigate the physiological and molecular mechanisms underlying the salinity tolerance regulated by GsCBRLK and provide valuable information for effective engineering strategies, GsCBRLK gene was transformed into soybean cotyledon explants. According to the PCR, RT-PCR and Western blot results, soybean transgenic lines with GsCBRLK inherited stably were generated. Phenotype and physiological characteristic ananlysis showed that GsCBRLK transgenic soybean plants grew much better than untransformed soybean plants under 200 mM NaCl treatment. These findings have contributed to our knowledge of plant salt tolerance mechanisms mediated by GsCBRLK. | |||
TO cite this article:LI Sheng,JI Wei. Overexpression of GsCBRLK gene enhanced salt tolerance of soybean plants[OL].[ 4 December 2015] http://en.paper.edu.cn/en_releasepaper/content/4669119 |
7. Genome structure analysis and the phylogenetic distribution of conifers | |||
Zhihua Liu | |||
Biology 25 November 2015 | |||
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Abstract:Conifers have diversified organizations of chloroplast genomes (cpDNAs), especially the ones with the two extremely reduced copies of the inverted repeat (IR). As the largest and the basal family of conifers, Pinus and Cryptomeria cpDNAs provide key insights into the evolutionary history of conifers. To reveal the various genomic organizations of conifers, main cpDNAs of conifers were utilized in comparative research. Pinus and Cryptomeria cpDNAs represented two types of IR structure, indicating that the cpDNA organization was very diverse in conifer plants. The availability of the conifer cpDNAs provided valuable information on the molecular phylogenetic study of conifers. | |||
TO cite this article:Zhihua Liu. Genome structure analysis and the phylogenetic distribution of conifers[OL].[25 November 2015] http://en.paper.edu.cn/en_releasepaper/content/4665755 |
8. Flower morphology and development of the monotypic Chinese genus Anemoclema (Ranunculaceae) | |||
ZHAO Liang | |||
Biology 20 November 2015 | |||
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Abstract:The systematic affinities of Anemoclema and its taxonomic rank within tribe Anemoneae of Ranunculaceae have long been in dispute. Anemoclema glaucifolium has traditionally been treated according to morphology within Anemone (including the Pulsatilla group), where it may represent a transition from Anemone towards Clematis or as its own, monotypic genus. However, the most recent molecular phylogenetic studies showed that Anemoclema was more closely related to Clematis than to Anemone. In order to help resolve the position of Anemoclema, we used light and scanning electron microscopy to study the morphology and development of the flower of Anemoclema glaucifolium. Our results support a close affinity of Anemoclema within Anemoneae on the basis of several floral developmental and morphological features: (1) broad and crescent-shaped young sepals, (2) narrow and rounded stamen primordia, (3) carpels ascidiate, stigma papillate, decurrent, (4) carpels bearing one median fertile ovule and a few lateral sterile ovules, (5) the fertile ovule appearing before the carpel closes, and (6) anatropous and unitegmic ovule. At the same time, Anemoclema also shared some characteristics with Clematis that are not present in Anemone: (1) stem leaves opposite (vs. stem leaves absent), (2) centripetal anther maturation (vs. centrifugal or bidirectional), and (3) conspicuous appendage at the ventral base of the ovule funicle (vs. inconspicuous appendage). Thus, our findings support the placement of Anemoclema within the tribe Anemoneae and its closer relationship to Clematis than to Anemone on the basis of floral morphogenesis. These conclusions are corroborated by previous molecular phylogenetic studies. | |||
TO cite this article:ZHAO Liang. Flower morphology and development of the monotypic Chinese genus Anemoclema (Ranunculaceae)[OL].[20 November 2015] http://en.paper.edu.cn/en_releasepaper/content/4664037 |
9. Isolation and characterization of LcSAP, a Leymus chinensis gene which enhances the salinity tolerance of Saccharomyces cerevisiae | |||
LIU Jingying,YANG Xizhe,DONG Hui | |||
Biology 07 September 2015 | |||
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Abstract:The PCR RACE method was used to isolate LcSAP from Leymus chinensis. The 17.6 kDa LcSAP product comprises 161 residues. RT-PCR analysis showed that LcSAP was more strongly transcribed in the leaf than in the root, and that it was up-regulated by the imposition of salinity stress. When expressed heterologously in brewers' yeast, the gene enhanced the yeast cells' salinity tolerance. | |||
TO cite this article:LIU Jingying,YANG Xizhe,DONG Hui. Isolation and characterization of LcSAP, a Leymus chinensis gene which enhances the salinity tolerance of Saccharomyces cerevisiae[OL].[ 7 September 2015] http://en.paper.edu.cn/en_releasepaper/content/4653399 |
10. Floral organogenesis in Urophysa rockii, a rediscovered, endangered and rare species of Ranunculaceae | |||
LIANG Zhao | |||
Biology 06 June 2015 | |||
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Abstract:Urophysa is an Asian endemic genus in the Ranunculaceae, but flora organogenesis data, which will be a useful complement to molecular data in clarifying the relationship with close related taxa (Aquilegia and Semiaquilegia) in Ranunculaceae, are completely lacking. We used scanning electron microscopy and light microscopy to study floral development of Urophysa rockii, a recently rediscovered species in this genus. The sepals are initiated spirally, while other organs are non-simultaneously whorled. The floral phyllotaxis is whorled. Primordia of the sepals are lunular and truncate, but those of the petals and stamens are hemispherical, rounded, and much smaller than the sepal primordia. After petal initiation, there is a delay in development. The nectariferous tissue is in the petal spurs. The development sequence of the microspores in the stamens is centrifugal, although the stamens are initiated centripetally. U. rockii has ten orthostichies (vertical rows of organs), with alternate orthostichies either sepal based or petal based. Scale-like staminodes terminate the orthostichies of the stamens. Carpels terminate the sepal-based orthostichies. The early developmental stages of the staminodes are similar to those of stamens, although much smaller, so they may be phylogenetically homologous organs. The carpel primordia are initiated in a single whorl, and they are lunular in shape and plicate. The stigma is everted and decurrent with unicellular papillae. The mature ovule is anatropous and bitegmic. Urophysa shows similar floral development features as Aquilegia and Semiaquilegia, although with some differences, which supports their relationship inferred by DNA sequence data. | |||
TO cite this article:LIANG Zhao. Floral organogenesis in Urophysa rockii, a rediscovered, endangered and rare species of Ranunculaceae[OL].[ 6 June 2015] http://en.paper.edu.cn/en_releasepaper/content/4645855 |
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