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1. Production of 1,4-butanediol from succinic acid using Escherichia coli whole-cell catalysis | |||
Ping Ni,Cong Gao,Jing Wu,Wei Song,Xiaomin Li,Wanqing Wei,Xiulai Chen,Liming Liu | |||
Biology 27 February 2024 | |||
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Abstract:The microbial production of 1,4-butanediol (1,4-BDO), a crucial bulk chemical used in the organic chemical and food industries, has attracted increasing attention due to environmental concerns. However, its biosynthetic efficiency via current biotransformation routes is limited. In this study, a dual-pathway approach for 1,4-BDO production from succinic acid was developed. Specifically, a double-enzyme catalytic pathway involving carboxylic acid reductase and ethanol dehydrogenase was proposed. Optimization of the expression levels of the pathway enzymes led to a significant 318% increase in 1,4-BDO titer. Additionally, the rate-limiting enzyme MmCar was engineered to enhance substrate affinity by 50% and increase 1,4-BDO titer by 46.7%. To address cofactor supply limitations, an NADPH and ATP cycling system was established, resulting in a 48.9% increase in 1,4-BDO production. Ultimately, after 48 hours, 1,4-BDO titers reached 201 mg/L and 1555 mg/L in shake flask and 5 L fermenter, respectively. This work represents a significant advancement in 1,4-BDO synthesis from succinic acid, with potential applications in the organic chemical and food industries. | |||
TO cite this article:Ping Ni,Cong Gao,Jing Wu, et al. Production of 1,4-butanediol from succinic acid using Escherichia coli whole-cell catalysis[OL].[27 February 2024] http://en.paper.edu.cn/en_releasepaper/content/4762275 |
2. A compound-hunting approach to reveal the amidohydrolase-dependent | |||
HAO Chunlin,YU Yi | |||
Biology 18 January 2014 | |||
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Abstract:In biosynthesis of natural products, potential intermediates or analogs of a particular compound in the crude extracts are commonly overlooked in routine assays due to their low concentration or because of limited structural information. This may lead into an incomplete and even an incorrect biosynthetic pathway for the target molecule. Here we established a compound-hunting approach which is based on genome mining and mass spectrometry and evaluated it to identify potential pyrrolamide compounds in the fermentation culture of Streptomyces netropsis. Several novel pyrrolamides were then detected and characterized, and a revised model for the biosynthesis of pyrrolamide compounds was proposed. Significantly, our results implied an unprecedented "iterative strategy" underlying the pyrrolamide antibiotics biosynthesis. Therefore, the approach developed in this study not only can direct scaffold-oriented discovery of potential biosynthesis intermediates and analogs, but also has the potential to extend our knowledge of existing natural product biosynthesis mechanisms. | |||
TO cite this article:HAO Chunlin,YU Yi. A compound-hunting approach to reveal the amidohydrolase-dependent [OL].[18 January 2014] http://en.paper.edu.cn/en_releasepaper/content/4582970 |
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