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1. Biosuppression of fungal decay in pear fruit by chitosan integration with biocontrol yeast and calcium chloride | |||
YU Ting,LU Huangping,ZHENG Xiaodong | |||
Agronomy 02 November 2011 | |||
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Abstract:This study evaluated the performance of chitosan at different concentrations alone, and in combination with a biocontrol yeast Cryptococcus laurentii and calcium chloride in control of the blue mold decay in pear fruit. The results showed that efficacy of chitosan in inhibiting blue mold decay in pear fruit decreased as the incubation time increased. Chitosan at 0.5% or above delayed the rapid growth of C. laurentii, nevertheless, it enhanced remarkably mold decay control when in combination with C. laurentii. Moreover, addition of calcium chloride the mixture of C. laurentii and chitosan further enhanced the decay control, although calcium chloride had little antifungal activity. Therefore, a combination of chitosan with C. laurentii and calcium chloride provides a great new potential for development of effective alternative approach to inhibit the blue mold decay especially in an integrated postharvest diseases management strategy. | |||
TO cite this article:YU Ting,LU Huangping,ZHENG Xiaodong. Biosuppression of fungal decay in pear fruit by chitosan integration with biocontrol yeast and calcium chloride[OL].[ 2 November 2011] http://en.paper.edu.cn/en_releasepaper/content/4448268 |
2. Down regulation of pre-RNA processing gene Mamrd1 decreases growth and virulence in entomopathogenic fungus, Metarhizium anisopliae | |||
Cao Yueqing ,Li Kai ,Xia Yuxian | |||
Agronomy 02 December 2010 | |||
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Abstract:Mrd1 is one of trans-acting protein and play important role in Pre-RNA processing. In this report, we characterized Mamrd1 gene from Metarhizium anisopliae and studied its function in growth and virulence. Like most of fungal Mrd1 protein, Mamrd1 has five RNA binding domains. Hyphal biomass and conidial production of M. anisopliae reduced significantly when Mamrd1 was down-regulated. Mamrd1-RNAi strain exhibited an appearance of fluffier, thicker mycelia layer with fewer and longer branches than that of wild strain. Bioassay test against locust demonstrated that the virulence of Mamrd1-RNAi mutant was significantly decreased compared with control, possibly due to its decreased growth in hemolymph of the locust. These results indicated that Mamrd1 is required for growth and virulence of M.anisopliae. | |||
TO cite this article:Cao Yueqing ,Li Kai ,Xia Yuxian . Down regulation of pre-RNA processing gene Mamrd1 decreases growth and virulence in entomopathogenic fungus, Metarhizium anisopliae[J]. |
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