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1. Pathological lesion and the antigenic localization of highly pathogenic avian influenza virus in Sheldrake Ducks | |||
LIU Xiaojing,ZHENG Shimin,SUN Zhengyang,GAO Xueli,LIU Chaonan | |||
Animal Husbandry, Veterinary Medicine 14 May 2017 | |||
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Abstract:In this paper, to determine the pathogenicity and antigen distribution of China early H5N1 highly pathogenic avian influenza virus (HPAIV) A/GS/LN/45/04, the Sheldrake ducks were inoculated via the nostrils. Infected ducks appeared depression, orticollis and nystagmus clinical signs, hematoxylin and eosin (HE) stain found multiple organs showed different degrees of pathological changes. In addition, by Immuno-histochemistry (IHC) stain found that A/GS/LN/45/04 virus could duplicated in multiple organs, especially in brain and pancreas. These results could provide some important theoretical basis for the further study of Chinese AIVs. | |||
TO cite this article:LIU Xiaojing,ZHENG Shimin,SUN Zhengyang, et al. Pathological lesion and the antigenic localization of highly pathogenic avian influenza virus in Sheldrake Ducks[OL].[14 May 2017] http://en.paper.edu.cn/en_releasepaper/content/4733275 |
2. Effects of Bioefficacy of DL-2-Hydroxy-4-Methylthio-Butanoic Acid on Egg Production and Egg Quality in Laying Hens Fed on Medium or Low Nutrient Scheme | |||
Jianmei Wan,Jianping Wang,Xuemei Ding,Shiping Bai,Qiufeng Zeng,Huanwei Peng,Zhuowei Su,Yue Xuan,Keying Zhang | |||
Animal Husbandry, Veterinary Medicine 21 April 2017 | |||
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Abstract:Two experiments were carried out to investigate the effects of bioefficacy of DL-2-Hydroxy-4-Methylthio-Butanoic Acid (HMTBA) for layers fed on medium or low nutrient level diets under restricted feeding (Exp.1) or ad libitum feeding (Exp.2) on egg production and quality. In Exp.1, a total of 2000 layers were assigned to 10 treatments in a completely randomized design, with 10 replicates per treatment, whilst in Exp.2 there were 10 replicates (15 hens per replicate) of 10 treatments. In all experiments, the bioefficacy of HMTBA was set at 80%, 84%, and 88% (on weight basis). In Exp.1, Methionine deficiency significantly (P < 0.05) reduced egg weight and eggshell weight regardless of the dietary nutrient level. Bioefficacy of HMTBA had no influence (P > 0.05) on any egg production or egg quality parameters of hens when compared with DL-methionine (DLM) treatments. In Exp.2, egg production, ADFI, egg weight, egg mass and feed conversion ratio significantly declined due to methionine deficiency (P < 0.01). There was no difference (P > 0.05) between HMTBA and DLM on egg production and egg quality parameters within each dietary nutrient level. In conclusion, bioefficacy of HMTBA can be set at 88% on weight basis regardless of dietary nutrient level. | |||
TO cite this article:Jianmei Wan,Jianping Wang,Xuemei Ding, et al. Effects of Bioefficacy of DL-2-Hydroxy-4-Methylthio-Butanoic Acid on Egg Production and Egg Quality in Laying Hens Fed on Medium or Low Nutrient Scheme[OL].[21 April 2017] http://en.paper.edu.cn/en_releasepaper/content/4727788 |
3. Effects of methionine source and level on hepatic methionine and lipids metabolism in laying hens fed with low or moderate nutrient level diet under restricted feeding | |||
Jianmei Wan,Jianping Wang,Xuemei Ding,Shiping Bai,Qiufeng Zeng,Huanwei Peng,Zhuowei Su,Yue Xuan,Keying Zhang | |||
Animal Husbandry, Veterinary Medicine 20 April 2017 | |||
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Abstract:The study was carried out to investigate the effects of methionine source and level on hepatic methionine and lipid metabolism of commercial laying hens fed with low nutrient level (LN) or moderate nutrient level (MN) diets under restricted feeding. A completely randomized design was used. Within each nutrient level, there were 2 methionine sources (DL-methionine, DLM; DL-2-hydroxyl-4-methylthio-butiric acid, HMTBA) and 3 supplementation levels (LN: 0, 0.091 and 1%; MN: 0, 0.113 and 1%). A total of 2000 layers was allocated to 10 dietary treatments with 10 replicates for each. For hens fed LN diets: regardless of nutrient level, methionine source and level had no effects on concentration of Hcy in liver or plasma; hens treated with DLM had higher plasma Met and Tau concentration than those treated with HMTBA; when compared with the control group, adding methionine (HMTBA source) at 0.091% or 1% markedly increased the expression of GNMT; adding methionine (DLM source) at 1% significantly increased the expression of BHMT. Under the condition of feeding MN diets: plasma Met concentration significantly increased with methionine supplementation. Adding methionine (HMTBA source) at 0.113% or 1% significantly increased the expression of ADA and adding methionine (HMTBA source) at 0.113% significantly increased the expression of MS. When control diet was fed, hens fed LN diets had higher expression of BHMT and MS. When adding methionine (HMTBA source) at 1%, hens fed LN diets had higher expression of SAHH, MTHFR, CEBPβ, AdipoR2, INF-α and IL-1β. When adding methionine (DLM source) at 1%, feeding LN diet significantly increased the expression of TNF-α. In conclusion, methionine deficiency may increase the methionine regeneration by up-regulating the expression of genes involved in hepatic methionine metabolism. Under the condition of adding excess HMTBA, feeding low-nutrient diet may increase the folate-dependent pathway of methionine regeneration, SAMe-dependent transmethylation, fat synthesis and inflammatory cytokines in liver, and increase the potential risk of liver diseases. | |||
TO cite this article:Jianmei Wan,Jianping Wang,Xuemei Ding, et al. Effects of methionine source and level on hepatic methionine and lipids metabolism in laying hens fed with low or moderate nutrient level diet under restricted feeding[OL].[20 April 2017] http://en.paper.edu.cn/en_releasepaper/content/4727428 |
4. The relative bio-efficacy of DL-methionine and DL-methionine hydroxyanalogue calcium saltcompared to L-methionine in broilers | |||
Peng Jialong,Gao Jun,Bai Shiping,Luo Yuheng,Ding Xuemei,Zeng Qiufeng,Wang Jianping,Zhang Keying | |||
Animal Husbandry, Veterinary Medicine 20 April 2017 | |||
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Abstract:The relative bio-efficacy (RBE) of DL-methionine (DLM) and DL-methionine hydroxy analogue calcium salt (MHA-Ca) compared to L-Met was evaluated in this paper. A total of 2560 10-d-old Ross 308 male broilers were randomly allotted to 16 treatments with 8 replicates of 20 birds each.Birds were fed with either a corn-soybean meal-pea protein concentrate-based diet (Met:0.28,10-21d;0.26,22-42d with no methionine and cysteine supplementation), or methionine supplemented diets at0.03, 0.06, 0.10, 0.15, and 0.21% methionine from L-Met, or DLM, or MHA-Ca from 10 to 42 days of age based on the Met content (L-Met or DL-Met) or equal mass to MHA.The average daily gain (ADG), average daily feed intake (ADFI) and feed conversion ratio (FCR) in the period of 10 to 42 days were measured. Liver (at 42d) was collected to determine the redox state:H2O2, catalase (CAT), malondialdehyde (MDA), total glutathione (T-GSH), oxidized GSH (GSSG), reduced GSH (reGSH) and 8-hydroxyguanosine (8-OHDG). Supplement of Met elicited linear or quadratic improvements on ADG, ADFI and FCR with no different among the Met sources except FCR (P=0.0251, L-Met and DLM was better than MHA-Ca). The effect on carcass performance was similar to growth performance. Based on equal mass, the RBE of DLMand MHA-Ca to L-Met was 115.4and 87.6%,respectively based on ADG; 94.6 and 48.6%, respectively, based on FCR; 119.8% and 91.2%, respectively, based on breast weight; 107.4% and 79.5%, respectively, based on breast meat yield. The supplementation of methionine (regardless of source) increased the levelsof GSH (P<0.0001) and decreased the level of MDA(P<0.0001), CAT (P<0.0001) and 8-OHDG (P=0.0035) in broiler livers. Inconclusion, the RBE of DLM and MHA-Ca was 108% and 64% on a product base. MHA-Ca tended to have an advantage on anti-oxidative system which may partly compensate for the low efficiency on protein synthesis, while the relation between anti-oxidative ability and RBE needed further study. | |||
TO cite this article:Peng Jialong,Gao Jun,Bai Shiping, et al. The relative bio-efficacy of DL-methionine and DL-methionine hydroxyanalogue calcium saltcompared to L-methionine in broilers[OL].[20 April 2017] http://en.paper.edu.cn/en_releasepaper/content/4727112 |
5. Effect of Different Methionine Source on Lipid Metabolism in the Liver of Broilers during Different Growth Phase | |||
Peng Jialong,Wang Jianping,Bai Shiping,Ding Xuemei,Zeng Qiufeng,Luo Yuheng,Zhang Keying | |||
Animal Husbandry, Veterinary Medicine 19 April 2017 | |||
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Abstract:To investigate the effect of different methionine sources on lipid deposit and lipid metabolism related genes expression in the liver of broilers during different growth phase, two trails was conducted. A total of 1200 1-day-old male Ross 308 broiler chicks with similar body weight were randomly selected and allotted to 10 treatments with 6 replications of 20 birds in trail 1. Birds were fed with either a corn-soybean meal-pea protein concentrate-based diet (Met:0.28, 1-21d with no methionine and cysteine supplementation), or methionine supplemented diets at 0.10, 0.22 and 0.32% from L-methionine (LM), DL-methionine (DLM)or methionine hydroxyl analogue calcium Salt (MHA-Ca) from 1-21d based on the Met content (LM or DLM) or equal mass to MHA. In trail 2, a total of 2112 21-day-old chicks were randomly allotted to 12 treatments with 8 replications of 22 birds each. Birds were fed with methionine supplemented diets at 0.05, 0.10, 0.14 and 0.24% from LM, DLM or MHA-Ca from 22-42d. A treatment fed with a basal diet (Met: 0.28, 1-21d; 0.26%: 22-42d) from 1-42d was set as negative control. Met deficiency significantly increased liver EE in broilers during 1-21d (P<0.0001) and Met excess increased that during 22-42d (P<0.0001). Chickens fed LM supplemental diets had the highest liver EE and MHA-Ca the lowest regardless of growth phase (P<0.0001).Met level had a significant effect on adiponectin receptor2 (ADIPOR2), acyl-coenzyme A oxidase 1 (ACOX1), carnitine palmitoyltransferase (CPT1A), carbohydrate response element-binding protein (CHREBP), fatty acid synthase (FASN) and apolipoprotein (APOB) expression during 22-42d (P<0.05) while only affected ACOX1, CPT1Aand CHREBP expression during 1-21d (P<0.05). There was a difference on CPT1A(P=0.010) and CHREBP (P=0.007) expression among Met sources during 1-21d and the expression of CHREBP(P=0.009), FASN (P=0.014) and APOB (P=0.002) existed difference during 22-42d. In conclusion, Met deficiency and excess may increase liver lipid accumulation by suppressing lipid catabolism and transport related gene expression in broilers and MHA-Ca showed a good benefit for fatty liver. | |||
TO cite this article:Peng Jialong,Wang Jianping,Bai Shiping, et al. Effect of Different Methionine Source on Lipid Metabolism in the Liver of Broilers during Different Growth Phase[OL].[19 April 2017] http://en.paper.edu.cn/en_releasepaper/content/4727133 |
6. Monochromatic green light stimuli during incubation accelerates posthatch pectorals muscle growth by altering the expression of myogenic genes and wnt signaling pathway of embryos and neonatal broilers | |||
ZHANG Lin,LI Jiaolong,WANG Xiaofei,ZHU Xudong,GAO Feng | |||
Animal Husbandry, Veterinary Medicine 18 February 2017 | |||
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Abstract:Previous studies have indicated that monochromatic green light-emitting diodes (LED) light stimuli during embryogenesis accelerated post-hatch pectoral muscle growth of broilers. In this study, we further investigated the potential mechanism of this phenomenon by determine the gene expression of paired box genes, myogenic regulatory factors and the key factors of Wnt/β-catenin signaling pathway in the pectoral muscle of embryos and neonatal broilers incubated in dark condition or green LED light stimulation. Arbor Acres fertile broiler eggs (n = 200) were randomly assigned to 2 incubation groups and exposed to continuous green light, or a dark condition (control) from the first day to 19 d of incubation. The light sourced from LED lamps with the intensity of 30 lx at eggshell level. The results showed that green LED light stimuli during embryogenesis increased the total pectorals muscle weight on days H3 and H7, and relative pectorals muscle weight of newly hatched chicks on day H7 (P < 0.05) in comparison to the dark condition. The expression of Pax3 on days E15 (P < 0.05) and E17 (P < 0.01), Pax7 on days E19 and H1 (P < 0.05), MyoD on days E19 and H1(P < 0.05), MyoG on day H1 (P < 0.05), and Myf5 from days E17 to H1 (P < 0.05) was higher in green light group than those in dark condition. In addition, although the no significant difference on Wnt11 mRNA expression was found between treatments, the green LED light stimuli upregulated the mRNA expression of Wnt3a form days E15 to E19, and β-catenin on day E19 (P < 0.05). These results suggest that providing 30 lx green LED light stimuli from the first day to 19 d of incubation may accelerate pectoral muscle growth in embryos and neonatal broilers by enhancing myoblast proliferation and differentiation in the late embryonic stage of embryos and neonatal broilers, which is potentially mediated through the transcriptional upregulation of Pax3, Pax7, MyoD, MyoG and Myf5 and the key factors (Wnt3a and β-catenin) of Wnt/β-catenin signaling pathway.. | |||
TO cite this article:ZHANG Lin,LI Jiaolong,WANG Xiaofei, et al. Monochromatic green light stimuli during incubation accelerates posthatch pectorals muscle growth by altering the expression of myogenic genes and wnt signaling pathway of embryos and neonatal broilers[OL].[18 February 2017] http://en.paper.edu.cn/en_releasepaper/content/4719405 |
7. Effects of different forms and concentrations of selenium on ConA induced T-cell proliferation in primary cultured pig splenocytes | |||
Chen Xingxiang,Ren Fei,Gan Fang,Shi Xiuli,Li Junxian,Huang Kehe | |||
Animal Husbandry, Veterinary Medicine 24 November 2016 | |||
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Abstract:To better understand the role of selenium (Se) in immunity, a few studies have been explored in some immune cells in vitro, but the effects of different forms of selenium on primary cultured pig splenocytes remain largely unclear. In present study, primary cultured pig splenocytes were incubated with 0 (control) and vary concentrations of selenium (Se) as selenomethionine (SeMet), or sodium selenite (Na2SeO3) for 48 h in the presence of ConA. Compared to controls, significantly higher T-cell proliferation were observed in groups treated by 0.5-32 μM SeMet, while T-cell proliferation were significantly increased in Na2SeO3-treated splenocytes at 0.5-4 μM, with maximal effects at 2 μM, and inhibited in a dose-dependent manner at 8-32 μM Na2SeO3. Intracellular reduced glutathione (GSH) contents and GPx activity in all Se-treated splenocytes were significantly higher than those in controls. An increases of GPx1 mRNA level were obtained in all Se-treated splenocytes, but GPx4 mRNA level was not affected by Se treatment. And mercaptosuccinic acid, the inhibitor of the GPx1, significantly inhibited the proliferation improved by Se. We conclude that regulations of ConA-induced T-cell proliferation by different Se forms are different in primary cultured pig splenocytes, and GPx1 maybe the key enzyme. Our finding may contribute to understanding the differential influences of Se in different forms on various types of immune responses. | |||
TO cite this article:Chen Xingxiang,Ren Fei,Gan Fang, et al. Effects of different forms and concentrations of selenium on ConA induced T-cell proliferation in primary cultured pig splenocytes[OL].[24 November 2016] http://en.paper.edu.cn/en_releasepaper/content/4711505 |
8. Selenomethionine alleviates the AFB1-induced damages in primary cultured chicken hepatocytes by improving SelW expression to inhibit CYP450 1A5 expression | |||
Chen Xingxiang,Che Chaoping,Gan Fang,Pan Cuiling,Huang Kehe | |||
Animal Husbandry, Veterinary Medicine 24 November 2016 | |||
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Abstract:The aim of this study was to evaluate the effects of selenomethionine (SeMet) on the hepatotoxicity induced by Aflatoxin B1 (AFB1) in primary cultured chicken hepatocytes and explore its mechanism involved. The results revealed that the toxic effect of AFB1 on chicken hepatocytes is dose dependent and AFB1 at concentration of over 0.05 μg/mL caused significant increases in the levels of malondialdehyde and cytochrome P450 enzymes (CYP450 1A5) mRNA and reduction on the levels of intracellular glutathione (GSH) and total superoxide dismutase in hepatocytes when compared with the control (P < 0.05). Supplement with 2 μmol/L of SeMet exerted protective effect against the AFB1-induced damage in chicken hepatocytes by significantly increasing the mRNA level of Selenoprotein W (SelW) and decreasing the mRNA level of CYP450 1A5 (P < 0.05). Knockdown of SelW with SelW-specific siRNA significantly increased the mRNA and protein levels of CYP450 1A5 (P < 0.05) and further attenuated the protective effect of SeMet on the AFB1-induced damage. Thus, it can be concluded that selenomethionine alleviates the AFB1-induced damage in primary cultured chicken hepatocytes by improving SelW expression to inhibit CYP450 1A5 expression. | |||
TO cite this article:Chen Xingxiang,Che Chaoping,Gan Fang, et al. Selenomethionine alleviates the AFB1-induced damages in primary cultured chicken hepatocytes by improving SelW expression to inhibit CYP450 1A5 expression[OL].[24 November 2016] http://en.paper.edu.cn/en_releasepaper/content/4711502 |
9. The Prolonged Effect of Glucagon-like Peptide 2 on Intestinal Mucosal Barrier Injury in Lipopolysaccharide-Challenged Weaned Piglets | |||
Deng Qiuhong,Jia Gang,Zhao Hua,Tang Jiayong,Chen Xiaoling,Liu Guangmang,Wang Kangning | |||
Animal Husbandry, Veterinary Medicine 16 June 2016 | |||
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Abstract:The prolonged protective effect of glucagon-like peptide-2 (GLP-2) on lipopolysaccharide (LPS)-induced intestinal injury was studied in weaned piglets, and a possible mechanism is proposed. Thirty 28-day-old weaned castrated piglets with initial BW of 6.8 ± 0.4 kg were randomly allotted to three groups: (i) non-challenged control; (ii) LPS-challenged control; (iii) LPS + high GLP-2. Piglets in groups (ii) and (iii) were s.c. injected with PBS supplemented with human [Gly2]GLP-21-34 at doses of 0 or 10 nmol/kg BW per day ,respectively, for seven consecutive days. Piglets were challenged with i.p. administration of Escherichia coli LPS at a dose of 100 μg/kg on d 14 to induce intestinal damage. Twenty-four hours later, the piglets were euthanized and intestinal tract samples were collected. On d 7 after weaning, GLP-2 treatment prevented weaning stress-induced increases in the plasma levels of LPS (P < 0.05), D(-)-lactate (P < 0.05), and diamine oxidase (DAO) activity (P < 0.05), as well as the increase in serum levels of pro-inflammatory cytokines (P < 0.05). On d 14, LPS challenge dramatically increased the barrier permeability (P < 0.05) and increased pro-inflammatory cytokines expression in the small intestines (P < 0.05) (except tumor necrosis factor?α (TNF-α) expression in the ileum) (P > 0.05) and cytokines levels in serum (P < 0.05). Lipopolysaccharide disrupted the ultrastructure of tight junction proteins, and decreased the expression of zona occluden-1 (ZO-1) (P < 0.05) and occludin (P < 0.05). Glucagon-like peptide 2 pretreatment reduced the serum level (P < 0.05) and mRNA expression of pro-inflammatory cytokines in the small intestines (P < 0.05) and abrogated the destructive effect of LPS on barrier permeability by restoring the expression and localization of tight junction proteins (P < 0.05). Glucagon-like peptide 2 significantly prevented the LPS-induced increase in the expression of myosin light-chain kinase (MLCK) (P < 0.05) and the increase in phosphorylated myosin light chain (pMLC) levels in the duodenum, jejunum, and ileum. These results indicate that GLP-2 pretreatment alleviates intestinal barrier injury and inflammation in LPS-challenged weaned piglets. The protective effect of GLP-2 involves: 1) stimulation of the expression of tight junction proteins and 2) inhibition of the redistribution of tight junction proteins by down-regulating the activation of the MLCK/pMLC pathway. | |||
TO cite this article:Deng Qiuhong,Jia Gang,Zhao Hua, et al. The Prolonged Effect of Glucagon-like Peptide 2 on Intestinal Mucosal Barrier Injury in Lipopolysaccharide-Challenged Weaned Piglets[OL].[16 June 2016] http://en.paper.edu.cn/en_releasepaper/content/4697695 |
10. Gambogic Acid Inhibit Hsp90 Expression and Restored growth plate in Thiram Induced Tibial Dyschondroplasia | |||
LI Jiakui,HUANG Shucheng | |||
Animal Husbandry, Veterinary Medicine 27 May 2016 | |||
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Abstract:Tibial Dyschondroplasia (TD) is an important leg problem in fast growing birds that disturbs the proximal growth plate; First time study was conduct to evaluate effect of Gambogic acid (GA) on Hsp90 expression and antioxidant capability in thiram induced Tibial dyschondroplasia. One hundred and fifty commercial chicks were distributed into three groups: Control (A), Thiram induced (B) and GA treated (C). Samples were collected at day 7 & 14 at end of experiment liver samples were collected to determine content of antioxidant enzymes. The results demonstrated that Hsp90 were highly expressed significantly (P>0.05) in thiram induced chicks that contributing to the development of TD and decrease in antioxidant capacity of liver. GA administration down regulated Hsp90 expressions levels and restore antioxidant capacity of liver significantly (P>0.05). In conclusion, GA return growth plate vascularization in TD affected chicks; it provides new clinical usage and protective role of this promising agent against TD. | |||
TO cite this article:LI Jiakui,HUANG Shucheng. Gambogic Acid Inhibit Hsp90 Expression and Restored growth plate in Thiram Induced Tibial Dyschondroplasia[OL].[27 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4694094 |
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