Authentication email has already been sent, please check your email box: and activate it as soon as possible.
You can login to My Profile and manage your email alerts.
If you haven’t received the email, please:
|
|
There are 2 papers published in subject: > since this site started. |
Results per page: |
Select Subject |
Select/Unselect all | For Selected Papers |
Saved Papers
Please enter a name for this paper to be shown in your personalized Saved Papers list
|
1. The translocation of CIDEC in hepatocytes depends on nutritional status | |||
Zhou Lei,Li Hongqiang,Yang Zaiqing | |||
Animal Husbandry, Veterinary Medicine 15 January 2014 | |||
Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
Abstract:The CIDEC protein is located in lipid droplets (LDs) and the endoplasmic reticulum (ER), and is induced in fatty liver. However, the binding domain, the functional domain and the underlying mechanism of CIDEC in stimulating fat accumulation remain unclear. Here, we investigated the subcellular localization and function of pig CIDEC and confirmed CIDEC promotes unilocular development of LDs, reduces the specific surface area (SSA) of LDs and stimulates fat accumulation in HepG2 cells. By analyzing a series of CIDEC mutants, we revealed the N-domain (1-173 aa) is involved in LD localization and the C-domain (174-238 aa) is necessary for LD fusion. Further analysis indicated that the 106-173 aa region includes an ER-binding domain. Moreover, CIDEC stayed in the ER under lipid deficient conditions and translocated to LDs under fatty acid stimulation suggesting that localization of CIDEC in the ER is prior to the LD. Conclusions: Our data indicated excessive nutrition stimulated hepatic CIDEC expression and an increasing level of CIDEC induced hepatic LD fusion and fat accumulation. Our work suggests that CIDEC protects LDs by decreasing the SSA of LDs and is involved in the development of fatty liver. | |||
TO cite this article:Zhou Lei,Li Hongqiang,Yang Zaiqing. The translocation of CIDEC in hepatocytes depends on nutritional status[OL].[15 January 2014] http://en.paper.edu.cn/en_releasepaper/content/4582429 |
2. Regulation of expression and activity of selenoenzymes by different forms and concentrations of selenium in primary cultured chicken hepatocytes | |||
Wu Xianshi,Wei Chengwu,Pan Cuiling,Duan Ying,Huang Kehe | |||
Animal Husbandry, Veterinary Medicine 09 November 2010 | |||
Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
Abstract:In this study, the effects of different forms and concentrations of Se on the regulation of glutathione peroxidase (GPx) activity and mRNA levels of phospholipid hydroperoxide GPx (GPx4) and type I deiodinase (D1) in chicken hepatocytes were evaluated. Primary cultured chicken hepatocyte monolayers derived from male White Leghorn chickens (aged 30-40 days) were incubated for 24 h with 0 (control), 0.5, 1, 1.5, 2, 3, 4 or 5 μmol/l of Se from DL-selenomethionine (Se-Met), Kappa-selenocarrageenan (Se-Car) or sodium selenite (Na2SeO3). Compared with controls, significant increases of GPx activity were observed in all hepatocytes treated with Se except for the hepatocytes treated with 5 μmol/l of Na2SeO3, with maximal effects at 2 μmol/l of Se-Met or Se-Car and 1.5 μmol/l of Na2SeO3, respectively. Significant decreases of GPx4 mRNA were observed in all hepatocytes treated with Se (vs. control). D1 mRNA was significantly increased in all groups treated with Se (vs. control), with maximal effects at 1.5 μmol/l of Se-Met and 0.5 μmol/l of Se-Car or Na2SeO3, respectively. Se-Met at doses of 1.5-5 μmol/l had greater effect on D1 mRNA than Se-Car and Na2SeO3 at equivalent doses. After resulting in the maximal effect, higher Se supplementation led to a dose-dependent reduction of GPx activity and D1 mRNA in all hepatocytes treated with Se. These results suggest that in chicken hepatocytes, the regulations of GPx and D1 of by different forms and concentrations of Se vary. | |||
TO cite this article:Wu Xianshi,Wei Chengwu,Pan Cuiling, et al. Regulation of expression and activity of selenoenzymes by different forms and concentrations of selenium in primary cultured chicken hepatocytes[J].British Journal of Nutrition,2010,104(11):1605-1612. |
Select/Unselect all | For Selected Papers |
Saved Papers
Please enter a name for this paper to be shown in your personalized Saved Papers list
|
Results per page: |
About Sciencepaper Online | Privacy Policy | Terms & Conditions | Contact Us
© 2003-2012 Sciencepaper Online. unless otherwise stated