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1. In vitro culture and induced differentiation of goat fetal lung-derived mesenchymal stem cells (LMSC) | |||
Gao Jing,Liu Pengxia,Tai Dapeng,Cang Ming,Liu Dongjun | |||
Animal Husbandry, Veterinary Medicine 24 February 2014 | |||
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Abstract:Mesenchymal stem cells (MSCs) occur in a variety of mammalian fetal and adult tissues. To date, most studies have been carried out on bone marrow-derived MSCs, with few studies reported on fetal lung-derived MSCs (LMSC). The purpose of this study was to separate fibroblastic cells from goat fetal lung tissue; culture these cells in vitro; identify their MSC characteristics; induce differentiation of goat fetal LMSCs into osteoblasts, adipocytes, chondrocytes, islet β-cells, and neurons; and validate their pluripotency. Our findings confirmed that the separated goat fetal LMSCs were fibroblasts capable of efficient growth and proliferation. RT-PCR showed positive expression of the following MSC marker genes compared to the control group: CD13, CD29, CD44, CD90, and CD106, and negative expression of CD45. Induced differentiation of goat fetal LMSCs into adipocytes, osteoblasts, chondrocytes, islet β-cells, and neurons was determined by cytochemical staining methods using Oil Red-O, silver nitrate (AgNO3), alcian blue, dithizone, and neuron-specific enolase (NSE), respectively; and confirmed by RT-PCR identification of the marker genes: PPARγ2, collagen I, COL2A1, PDX1, and neuron-specific NSE, respectively. In conclusion, this study showed that goat fetal lung-derived cells possessed the biological characteristics and multipotent differentiation potential of MSCs. In addition, we have established successful methods for the separation, purification, and validation of goat fetal LMSCs, thereby preparing the ground for further cell cloning therapies and associated animal model studies. | |||
TO cite this article:Gao Jing,Liu Pengxia,Tai Dapeng, et al. In vitro culture and induced differentiation of goat fetal lung-derived mesenchymal stem cells (LMSC)[OL].[24 February 2014] http://en.paper.edu.cn/en_releasepaper/content/4587206 |
2. Evaluation of reference genes for investigation of gene expression profiles in chorioallantois from Meishan and Yorkshire pigs on gestational days 26 and 50 | |||
HOU Chunyan,YU Mei,LI Xiaoping | |||
Animal Husbandry, Veterinary Medicine 24 December 2013 | |||
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Abstract:Selection of suitable reference gene is important for accurate measurement of gene expression data obtained using the quantitative real-time PCR (qPCR) method. There has been no systematic investigation of reference genes for porcine chorioallantois which plays important roles in mediating concepts/fetus growth and survival. In this study, six candidate reference genes expressed in pig chorioallantois from Meishan and Yorkshire pigs on gestational days 26 (GD26) and 50 (GD50) were evaluated by qPCR. These genes included TATA box binding protein (TBP), glyceraldehydes-3-phosphate dehydrogenase (GAPDH), ribosomal protein L32 (RPL32), actin-beta (ACTB), ribosomal protein S20 (RPS20) and RNApolymerase polypeptide G (RPG). The expression stability of these genes was investigated by using the GeNorm software program and statistical analyses. The rank order of the expression stability for these genes was (from the most stable to the least stable): TBP, GAPDH, RPL32, ΒACTIN, RPS20 and RPG. Collectively, the results suggest that the TBP and GAPDH could be considered as the suitable reference genes for investigation of gene expression in the pig chorioallantois from the two breeds at different gestational stages. | |||
TO cite this article:HOU Chunyan,YU Mei,LI Xiaoping. Evaluation of reference genes for investigation of gene expression profiles in chorioallantois from Meishan and Yorkshire pigs on gestational days 26 and 50[OL].[24 December 2013] http://en.paper.edu.cn/en_releasepaper/content/4577871 |
3. Characterization and SNP Identification of Goat TYRP1 Gene | |||
Zheng Huiqin ,Lanhui Li,Xianglong Li,Rongyan Zhou,Fengling Xuan,Jingjing Zhang,Zunan Shen,Hongbo Zhao | |||
Animal Husbandry, Veterinary Medicine 20 April 2010 | |||
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Abstract:As one member of tyrosinase-related family, tyrosinase-related protein-1(TYRP1) has been reported to play an important role in melanin biosynthetic pathway in some species, but little is known about it in goat. In this study, almost complete goat TYRP1 gene (17554bp) encompassing complete CDS was determined, the coding region was 1614bp, distributed in 7 exons (2-8), intron 5 was found to possess GC-AG as its splice sites. Dozens of SNPs as well as simple sequence repeats (SSR) motifs were detected in the goat TYRP1 gene. Popgene analysis suggested that allele A at site 1263 and haplotype AC for g.1263A>C and g.1428C>T should be in favor of eumelanin biosynthesis, while mutation C→T at site 1428 occurred more recently in evolution and might have little effect on coat color. High deficiency of heterozygotes existed in Nanjiang Yellow Goat Black strain, Nanjiang Yellow Goat Fast Grow strain and Jining Gray Goat, indicating high inbreeding coefficient. Genetic differentiation among the 9 goat populations were significantly low for both g.1263A>C and g.1428C>T. | |||
TO cite this article:Zheng Huiqin ,Lanhui Li,Xianglong Li, et al. Characterization and SNP Identification of Goat TYRP1 Gene[OL].[20 April 2010] http://en.paper.edu.cn/en_releasepaper/content/42134 |
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