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1. Gambogic Acid Inhibit Hsp90 Expression and Restored growth plate in Thiram Induced Tibial Dyschondroplasia | |||
LI Jiakui,HUANG Shucheng | |||
Animal Husbandry, Veterinary Medicine 27 May 2016 | |||
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Abstract:Tibial Dyschondroplasia (TD) is an important leg problem in fast growing birds that disturbs the proximal growth plate; First time study was conduct to evaluate effect of Gambogic acid (GA) on Hsp90 expression and antioxidant capability in thiram induced Tibial dyschondroplasia. One hundred and fifty commercial chicks were distributed into three groups: Control (A), Thiram induced (B) and GA treated (C). Samples were collected at day 7 & 14 at end of experiment liver samples were collected to determine content of antioxidant enzymes. The results demonstrated that Hsp90 were highly expressed significantly (P>0.05) in thiram induced chicks that contributing to the development of TD and decrease in antioxidant capacity of liver. GA administration down regulated Hsp90 expressions levels and restore antioxidant capacity of liver significantly (P>0.05). In conclusion, GA return growth plate vascularization in TD affected chicks; it provides new clinical usage and protective role of this promising agent against TD. | |||
TO cite this article:LI Jiakui,HUANG Shucheng. Gambogic Acid Inhibit Hsp90 Expression and Restored growth plate in Thiram Induced Tibial Dyschondroplasia[OL].[27 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4694094 |
2. Selenium-Enriched Probiotics Improves Antioxidant Status, Immune Function and Selenoprotein Gene Expression of Piglets Raised under High Ambient Temperature | |||
Gan Fang,Chen Xingxiang,Shengfa F. Liao,Lv Chenhui,Ren Fei,Ye Gengping,Pan Cuiling,Huang Da,Shi Jun,Shi Xiuli,Zhou Hong,Huang Kehe | |||
Animal Husbandry, Veterinary Medicine 04 May 2014 | |||
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Abstract:This research was conducted to evaluate the effects of selenium-enriched probiotics (SP) on growth performance, antioxidant status, immune function and selenoprotein gene expression of piglets under natural high ambient temperature in summer. Forty-eight crossbred weanling piglets randomly allocated to four groups were fed for 42 days ad libitum a basal diet without (Con, 0.16 mg Se/kg) and with supplementation of probiotics (P, 0.16 mg Se/kg), sodium selenite (SS, 0.46 mg Se/kg) and SP (0.46 mg Se/kg). From each group, three piglets were randomly selected for blood collection on Days 0, 14, 28 and 42 and tissue collection on Day 42. The SP improved growth performance of piglets. Both SS and SP increased blood glutathione peroxidase activity and tissue thioredoxin reductase 1 mRNA expression with the SP being higher than the SS. All P, SS and SP supplementation increased the superoxide dismutase activity (40.1%, 53.0% and 64.5%), glutathione content (84.6%, 104% and 165%), TCR-induced T lymphocyte proliferation (20.8%, 26.4% and 50.0%), IL-2 concentration (24.9%, 27.2% and 46.2%), and decreased malondialdehyde content (25.1%, 26.3% and 49.3%), respectively. The greatest effects of SP supplementation suggest that SP may serve as a better feed additive than P or SS for piglets under high-temperature environments. | |||
TO cite this article:Gan Fang,Chen Xingxiang,Shengfa F. Liao, et al. Selenium-Enriched Probiotics Improves Antioxidant Status, Immune Function and Selenoprotein Gene Expression of Piglets Raised under High Ambient Temperature[OL].[ 4 May 2014] http://en.paper.edu.cn/en_releasepaper/content/4595834 |
3. Effects of feeding glycerol-enriched yeast product to transition dairy cow on lactation performance, blood metabolites, and rumen fermentation | |||
Ye Gengping,Liu Jin,Che Chaoping,Liu Yunhuan,Huang Kehe | |||
Animal Husbandry, Veterinary Medicine 04 May 2014 | |||
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Abstract:Forty-four transition Holstein cows were blocked by parity and expected calving date, and allocated to four treatments with a randomized complete block design to evaluate the effects of feeding glycerol-enriched yeast product (GYP) to transition dairy cow on lactation performance, blood metabolites, and rumen fermentation. Treatments were as follows: control (no additive), 2 L/d GYP (75.8 g/L glycerol and 15.3 g/L yeast), 150 g/d glycerol (G, 0.998 g/g glycerol), and 1 L/d yeast culture (Y, 31.1 g/L yeast). All additives were topdressed and hand-mixed into the upper one-third of total mixed ration in the morning feeding from -14 to +28 day relative to calving. Results indicated that dry matter intake, changes in body weights and body condition scores, as well as milk yield were not affected by treatments. However, cows fed GYP and Y had higher milk fat and milk protein percentages than control cows (P < 0.05). Compared with control, feeding GYP and G to cows increased the concentration of plasma glucose coupled with decreased concentrations of plasma beta-hydroxybutyrate acid and nonesterified fatty acid (P < 0.05). Additionally in ruminal parameters on 14 d postpartum, cows fed GYP and Y had higher total volatile fatty acids, and cows fed GYP and G had higher molar proportions of propionate than control cows (P < 0.05). In conclusion, GYP is a feasible feed additive of transition dairy cows, which can improve energy status and lactation performance and rumen fermentation via the effects of glycerol and yeast, respectively. | |||
TO cite this article:Ye Gengping,Liu Jin,Che Chaoping, et al. Effects of feeding glycerol-enriched yeast product to transition dairy cow on lactation performance, blood metabolites, and rumen fermentation[OL].[ 4 May 2014] http://en.paper.edu.cn/en_releasepaper/content/4595599 |
4. Feeding glycerol-enriched yeast culture improves performance, energy status, and heat shock protein gene expression of lactating Holstein cows under heat stress | |||
Liu Jin,Ye Gengping,Zhou Yuanli,Liu Yunhuan,Zhao Lina,Liu Yongjie,Chen Xingxiang,Huang Da,Liao Shengfa,Huang Kehe | |||
Animal Husbandry, Veterinary Medicine 04 May 2014 | |||
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Abstract:In this study,the effects of supplemental common yeast culture (CY) and glycerol-enriched yeast culture (GY) on performance, plasma metabolites, antioxidant status, and heat shock protein 70 (HSP70) mRNA expression were evaluated in lactating Holstein cows under heat stress. During summer months, 30 healthy multiparous lactating cows (parity 3.25 ± 0.48; 60 ± 13 d in milk [DIM]; 648 ± 57 kg BW; an average milk yield of 33.8 ± 1.6 kg/d) were blocked by parity, previous milk yield, and DIM and randomly allocated to 3 dietary treatments: no supplemental yeast culture (Control), 1 L/d of CY (33.1 g yeast) per cow, and 2 L/d of GY (153.2 g glycerol and 31.6 g yeast) per cow. During the 60-d experiment, values of air temperature and relativehumidity inside the barn were recorded hourly every 3 d to calculate temperature-humidity index (THI). Weekly rectal temperatures (RT) and respiration rates and daily DMI and milk yield were recorded for all cows. Milk and blood samples were taken twice monthly, and BW and BCS were obtained on d 0 and 60. In this experiment, THI values indicated cows experienced a moderate heat stress. Cows supplemented with CY and GY had greater yields of milk, energy-corrected milk and milk fat, and milk fat percent but lower HSP70 mRNA expression in peripheral blood lymphocytes than Control cows (P < 0.05). Supplementing CY and GY tended (P < 0.15) to decrease RT at 1400 h, increase milk protein yield and erythrocyte glutathione, and reduce plasma urea nitrogen compared with Control. Lower plasma NEFA concentration and HSP70 mRNA expression in peripheral blood lymphocytes (P < 0.05) and tendencies towards greater plasma glucose concentration (P = 0.11) but less BW loss (P = 0.14) were observed in GY relative to CY cows. In conclusion, either CY or GY supplementation partially mitigated the negative effects of heat stress on performance and HSP70 mRNA expression of lactating cows, and GY supplementation provided additional improvements in energy status and HSP70 gene expression of lactating cows. | |||
TO cite this article:Liu Jin,Ye Gengping,Zhou Yuanli, et al. Feeding glycerol-enriched yeast culture improves performance, energy status, and heat shock protein gene expression of lactating Holstein cows under heat stress[OL].[ 4 May 2014] http://en.paper.edu.cn/en_releasepaper/content/4595742 |
5. Recognition of fluoride neurotoxicity | |||
NIU Ruiyan,SUN Zilong,WANG Jundong | |||
Animal Husbandry, Veterinary Medicine 09 February 2014 | |||
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Abstract:In this paper, the first description of neurological symptoms in patients with fluorosis has hinted the neurotoxic potential of fluoride. The increasing number of epidemiological studies on fluoride neurotoxicity, especially on children's intelligence, provided evidence for the link between fluoride exposure and nervous system impairment. Meanwhile, the animal behavioral tests by various methods showed that fluoride not only affected spontaneous behaviors, but also impaired the learning and memory ability. Fluoride detected in brain indicated its ability to penetrate the blood-brain barrier. Accumulation of fluoride in brain resulted in biochemical and functional changes in the nervous system. In order to present the understanding process of fluoride neurotoxicity, this review summarize investigations from our and others groups which mainly focus on the effect of fluoride on the nervous system. | |||
TO cite this article:NIU Ruiyan,SUN Zilong,WANG Jundong. Recognition of fluoride neurotoxicity[OL].[ 9 February 2014] http://en.paper.edu.cn/en_releasepaper/content/4584911 |
6. Changes in miRNAs expression profiling in testis of mice exposure to Sulfur Dioxide and Fluoride | |||
ZHANG Jianhai,LIANG Chen,NIU Ruiyan,SUN Zilong,WANG Jundong | |||
Animal Husbandry, Veterinary Medicine 12 December 2012 | |||
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Abstract:MicroRNAs (miRNAs) are believed to play important roles in developmental and other cellular processes by hybridizing to complementary target mRNA transcripts, also involved in the development of mammalian spermatogenesis. Fluoride and sulfur dioxide are two well known environment pollutants, which present a serious threat to male reproduction. To compare the global miRNA expression profiling and regulation pattern in mouse testes between normal mouse and F or SO2-treat mice by using miRNA microarray technology, 144 male Kunming mice were divided randomly into four groups of thirty-six mice each. One group was left untreated as controls, and the other three groups were administered, respectively, for thirteen consecutive weeks, 150 mg NaF/L (45 mg F-/L) in their drinking water, SO2 in ambient air (26.2 mg/m3 SO2, 3hr/day), or were exposed to both NaF and SO2 together. After 15 weeks, testis totals RNA of the mouse model were gathered for miRNA microarray study. Simultaneity, reproductive capacity of mice, DNA contents distribution in testis cells, and bcl-2, bax, and p53 genes mRNA expression were detected to interpret the changed miRNA expression profiles. The results exhibited that MiR-106a in the NaF group, miR-449a, miR-16, miR-15b, and miR-34c in the SO2 groups, and miR-204 and miR-34a in the NaF+SO2 group were un-regulated significantly in testes of mice, compared with the controls, respectively. Whereas miR-34a decreased by 3.3-fold in the SO2 group, and miR-34b was down-regulated by more than 1.5-fold in the NaF+SO2 group. Further investigation indicates that predicted pathways according to significant miRNA are primary related to cell cycle, apoptosis, and MAPK signal pathway. Furthermore, the development of testis and reproductive capacity of male mouse have been weakened by F or SO2, especially, by F and SO2 together treatment. FCM analysis show that the percents of apoptosis cells in testis were increased significantly in experiment groups, DNA contents distributions have been disturbed by F or SO2. Expression of p53 and bcl-2 mRNA decreased, and bax gene was increased by1.87, 2.40 and 2.89 times as high as those in the control groups by QRT-PCR. However, both F or SO2 and the combination heighten the ratios of p53/bcl-2 and bax/bcl-2, all these changes lead to a common result of the spermatogenic cell apoptosis. In conclusion, changed miRNAs expression regulation should be look as the main mechanism of depressed male reproduction function of mouse induced by F or SO2 or their combination. This is the first miRNA microarray study to focus on evaluating altered miRNA expression profiles in testis of mice exposure to F or SO2. The miRNA microarray and bioinformatics analysis is a fast, high-throughout, exacts, foreground technique contribute toward elucidating the function of miRNAs in normal and impaired male reproduction by environment factors. | |||
TO cite this article:ZHANG Jianhai,LIANG Chen,NIU Ruiyan, et al. Changes in miRNAs expression profiling in testis of mice exposure to Sulfur Dioxide and Fluoride[OL].[12 December 2012] http://en.paper.edu.cn/en_releasepaper/content/4501514 |
7. The Application and Assessment of Ketamine–Xylidinothiazoline Combinations for Anaesthesia in Rhesus Monkey | |||
xiao jianhua,Wang Hongbin | |||
Animal Husbandry, Veterinary Medicine 07 January 2008 | |||
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Abstract:To access the different effects of between ketamine and ketamine-Xylidinothiazoline in anesthesia of rhesus monkeys. 20 Rhesus Macaque were anesthesia with tow kinds methods in this experiment. The anesthesia effects were evaluated during anesthesia. At the same time the cardiovascular parameters, and respiration parameters were monitored with Datex circulation, respiration monitor and multi-functional physiologic graphed SJ-42 continuously and dynamically. The results showed that the effects of anesthesia was good, it reach the surgical anesthetic depth. The relation between analgesia, sedation and muscle relaxation were balanced. The induction time were 5.0±1.6min and the anesthesia maintained 55.3±6.6min in all. Furthermore the compound anesthetic has little effects on the physiology function of Rhesus Macaque and heart rate, blood pressure, blood oxygen saturation, body temperature, respiratory rate maintain in normal physiological range during anesthesia. It was evident that the compound anesthetic can meet the clinical needs. | |||
TO cite this article:xiao jianhua,Wang Hongbin . The Application and Assessment of Ketamine–Xylidinothiazoline Combinations for Anaesthesia in Rhesus Monkey[OL].[ 7 January 2008] http://en.paper.edu.cn/en_releasepaper/content/17840 |
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