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1. Glutamine Supplementation Protects against Experimental Antibiotic-associated Diarrhea | |||
Mao Jinyan,Li Haitao | |||
Food Science and Technology 05 March 2019 | |||
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Abstract:Diarrhea is a major adverse effect of antibiotic use; however, no known medical regimen is available yet. Glutamine has a pivotal role in intestinal homeostasis, but its implication in antibiotic-associated diarrhea (AAD) remains unclear. Herein we explored the potential of glutamine supplementation in AAD management by using a preclinical mouse model. Data clearly showed that upon clindamycin exposure glutamine store was depleted in colonic mucosa, whereas glutamine supplementation improved several symptoms of experimental AAD. Mechanistically, clindamycin treatment substantially confounded gut microbiota especially in butyrate-producing strains, thereby leads to fecal butyrate deficiency, and in turn increases the body\'s demand for glutamine. Protective effect of glutamine supplementation on AAD may, at least in part, be explained by restoring the intestinal mucosal barrier and facilitating antimicrobial host defense. Taken together, our findings in this study provided a valuable rationale for guiding the clinical uses of glutamine in AAD management. | |||
TO cite this article:Mao Jinyan,Li Haitao. Glutamine Supplementation Protects against Experimental Antibiotic-associated Diarrhea[OL].[ 5 March 2019] http://en.paper.edu.cn/en_releasepaper/content/4747583 |
2. Development of Monoclonal Antibodies Suitable for Detection of Salmonella Enteritidis by Biosensor | |||
ZHANG Xiaoguang,WANG Zijie,ZHANG Zuomin,WU Pingping,LIU Shijie,ZHOU Hongru | |||
Food Science and Technology 15 April 2018 | |||
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Abstract:Monoclonal antibodies (MAbs) suitable for detection of Salmonella Enteritidis (SE) by biosensor were prepared and characterized. Indirect enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR) biosensor were used for screening of the hybridoma cells secreting MAbs specific to the pathogen. Based on the reactivity of MAbs against the target pathogen by SPR biosensor, MAbs were selected. The clones of 3-5H-3F and 3-7A-8B were found with high reactivity against boiled SE and very low against boiled S. Typhimurium and mixture samples including various non-target bacteria. These two clones were selected for detection of SE and anti-SE MAbs were purified from the culture supernatants. Results showed that MAbs selected via ELISA method, however, may not suitable for detection of target pathogen by using biosensor. The MAbs obtained after screening by the combined method showed a capacity to detect SE by using SPR biosensor as well as ELISA. These MAbs are useful for detection of SE by using biosensor and are expected to contribute to the development of rapid detection of SE in food samples. | |||
TO cite this article:ZHANG Xiaoguang,WANG Zijie,ZHANG Zuomin, et al. Development of Monoclonal Antibodies Suitable for Detection of Salmonella Enteritidis by Biosensor[OL].[15 April 2018] http://en.paper.edu.cn/en_releasepaper/content/4744606 |
3. Genistein Ameliorates Non-alcoholic Fatty Liver Disease by Targeting Thromboxane A2 Pathway | |||
Wang Wenzhe,Li Haitao, | |||
Food Science and Technology 09 March 2018 | |||
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Abstract:Non-alcoholic fatty liver disease (NAFLD) is now a public health issue worldwide, but no drug has received approval yet. Here we demonstrated that genistein, an isoflavonoid derived from soybean, effectively attenuated high fat diet-induced NAFLD progression by targeting thromboxane A2 (TXA2) pathway. Analysis of profile of eicosanoid synthesis pathways in liver unexpectedly revealed that TXA2 pathway was aberrantly active in NAFLD. Switching off TXA2 biosynthesis by aspirin effectively suppressed high-fat induced NAFLD progression. In parallel, genistein was identified as a natural thromboxane A2 receptor (TBXA2R) antagonist and successfully applied in NAFLD prevention. Phenotypically, treatment with either aspirin or genistein suppressed body weight gain, attenuated liver steatosis, normalized aminotransferase abnormalities and improved glucose tolerance. Further mechanism study suggested that TXA2 pathway was required for tumor necrosis factor-alpha (TNF-α) induced hepatic insulin resistance. Collectively, our findings established the critical pathophysiological role of the TXA2 pathway in NAFLD, and provided a rationale for introducing a strategy targeting TXA2 for NAFLD prevention and management. | |||
TO cite this article:Wang Wenzhe,Li Haitao,. Genistein Ameliorates Non-alcoholic Fatty Liver Disease by Targeting Thromboxane A2 Pathway[OL].[ 9 March 2018] http://en.paper.edu.cn/en_releasepaper/content/4743759 |
4. Immunomodulatory effects of Lactobacillus casei Zhang in a murine model of peanut allergy | |||
Mengsha Hu,Qiuxiang Zhang,Xiaoming Liu,Gang Wang,Hao Zhang,Wei Chen | |||
Food Science and Technology 14 March 2013 | |||
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Abstract:Probiotics can modulate systemic and mucosal immune systems, as well as induce immune tolerance in allergic diseases. The aim of this study was to evaluate the immunomodulatory potential of Lactobacillus casei Zhang on prophylactic and therapeutic effects in a mouse model of peanut allergy. Intragastrical administration with L. casei Zhang, prior or after allergic sensitization, led to increased levels of serum allergen-specific IgG2a and mucosal IgA antibodies. Additionally, systemic anaphylactic symptoms and Th2 responses (IgE antibody, histamine release and IL-4 production) were attenuated. L. casei Zhang had less influence on the serum IgG1 antibody and other cytokines (IL-10, IL-12 and IFN-γ). Successful immunomodulation by pre and post-treatment were further demonstrated by an augment of the IFN-γ/IL-4 ratio in vitro and enrichment of CD4+ Foxp3+ Tregs in spleen and mesenteric lymph nodes. These results suggested that L.casei Zhang may play a role in preventing and treating peanut allergy. | |||
TO cite this article:Mengsha Hu,Qiuxiang Zhang,Xiaoming Liu, et al. Immunomodulatory effects of Lactobacillus casei Zhang in a murine model of peanut allergy[OL].[14 March 2013] http://en.paper.edu.cn/en_releasepaper/content/4530123 |
5. In vitro screening of lactobacilli with antagonistic activity against Campylobacter jejuni growth and infection in human intestinal epithelial cells | |||
Yu Zhao,Fengwei Tian,Haiqin Chen,Xiaoming Liu,Gang Wang,Qiuxiang Zhang,Jianxin Zhao,Hao Zhang,Wei Chen | |||
Food Science and Technology 19 March 2012 | |||
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Abstract:Campylobacter jejuni is identified as the leading bacterial pathogen causing enterocolitis in human, usually leading to watery or bloody diarrhea, abdominal pain. Previous studies reported that some lactic acid bacteria have inhibitory effects on pathogenic bacteria, however the protective effects of lactobacilli strains on infection with C.jejuni have not been extensively studied. In this study, seventy-eight lactobacilli strains were screened for anti-C.jejuni activity using well diffusion agar assays and the ability of adhesion to human intestinal epithelial (HT-29) cell were also assessed. Four lactobacilli strains, i.e, strain N8, N9, ZL4 and ZL5, showed obvious potential anti-C.jejuni growth in vitro as well as their significant ability of adhesion to the HT-29 cell. Furthermore, these four lactobacilli strains showed significant potential for reducing adhesion and invasion of C.jejuni to HT-29 cell in exclusion, competition and displacement assays, respectively. All the four strains also performanced a desired resistance to the simulated gastrointestinal conditions. Taken together, this study suggests that lactobacilli strains, N8, N9, ZL4 and ZL5 could be used as potential probiotics for prevention infection with C.jejuni . | |||
TO cite this article:Yu Zhao,Fengwei Tian,Haiqin Chen, et al. In vitro screening of lactobacilli with antagonistic activity against Campylobacter jejuni growth and infection in human intestinal epithelial cells[OL].[19 March 2012] http://en.paper.edu.cn/en_releasepaper/content/4472323 |
6. Sinusoid envelope voltammetry - A novel voltammetry waveform for electronic tongue | |||
Tian Shiyi,Xiao Xiao,Deng Shaoping | |||
Food Science and Technology 22 January 2012 | |||
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Abstract:A novel voltammetry for voltammetric electronic tongue so called sinusoid envelope voltammetry was introduced in this paper. Fourier transformation was used to transform the data of its response signal from the time domain to the frequency domain. Four taste substances, i.e. acesulfame potassium, monosodium glutamate, potassium chloride and tartaric acid, exhibited abundant frequency characters in the power spectrum of Fourier transformation respectively, which indicated that the power spectrum from sinusoid envelope voltammetry could be used as fingerprints of samples for classification. Also, principal component analysis with discrimination index and multi-frequency large amplitude pulse voltammetry as a reference technique was used to evaluate the separation ability of sinusoid envelope voltammetry. The score plot of sinusoidal envelope voltammetry from four taste substances and five brands of Jiafan rice wine showed a better discrimination ability than multi-frequency large amplitude pulse voltammetry. Sinusoid envelope voltammetry would be a promising technology for voltammetric electronic tongue. | |||
TO cite this article:Tian Shiyi,Xiao Xiao,Deng Shaoping. Sinusoid envelope voltammetry - A novel voltammetry waveform for electronic tongue[OL].[22 January 2012] http://en.paper.edu.cn/en_releasepaper/content/4463707 |
7. Application of response surface methodology for extraction optimization of the water-soluble polysaccharide from Pteridium aquilinum | |||
XU Wen-Tao,ZHANG Fang-Fang,Huang Kunlun,Wang Ying,Luo Yunbo | |||
Food Science and Technology 08 April 2008 | |||
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Abstract:The optimum conditions for the extraction of the water-soluble polysaccharide from Pteridium aquilinum were determined using response surface methodology (RSM). A central-composite experimented design (CCD) was used to investigate the effects of three independent variables, namely liquid: solid ratio, extraction temperature and extraction time. Also, the antioxidative activity of the crude polysaccharide obtained from Pteridium aquilinum was investigated in this study. The optimum conditions were determined as a liquid: solid ratio of 18.8:1 (v/w), an extraction temperature of 62.5 ℃ and an extraction time of 5.9h. The crude polysaccharide showed better antioxidiative activity by FARP antioxidative assay. | |||
TO cite this article:XU Wen-Tao,ZHANG Fang-Fang,Huang Kunlun, et al. Application of response surface methodology for extraction optimization of the water-soluble polysaccharide from Pteridium aquilinum[OL].[ 8 April 2008] http://en.paper.edu.cn/en_releasepaper/content/20202 |
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