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1. Expression profiling of microRNAs in hippocampus of rats following traumatic brain injury | |||
SUN Tingyi,LIU Zilong,LIU Liang,CHEN Xiaorui,ZHAO Lili,QU Guoqiang | |||
Clinical Medicine 28 June 2013 | |||
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Abstract:Objectives To descript the changes in expression of microRNAs in the hippocampus of rats at 1 day, 3 days and 5 days following traumatic brain injury (TBI) by microarray technique, and investigate the possible cellular activities that are regulated by microRNAs which differentially expressed in the hippocampus, that may contribute to TBI-induced cognitive impairment. Methods Adult SD rats received a single controlled cortical impact (CCI) injury using an electromagnetic piston at a velocity of 4 m/sec, duration of 150 ms and depth of 3 mm. The ipsilateral hippocampus was harvested for the subsequent microarray assays at three time points post-injury: 1 day, 3 days and 5 days, respectively. We characterize the microRNA expression profile in rat hippocampus compared with sham group using the Significance Analysis of Microarrays analysis. Results We got a dendrogram of a Hierarchical Clustering analysis of the result. Totally 205 microRNAs were identified which were up-/down-regulated more than 1.5 times. At 1 d post-injury, 41 microRNAs were up-regulated more than 1.5 times, and 31 microRNAs were down-regulated more than 1.5 times; at 3 d post-injury, 81 microRNAs were up-regulated more than 1.5 times, and 11 microRNA s were down-regulated more than 1.5 times; at 5 d post-injury, 52 microRNAs were up-regulated more than 1.5 times, and 41 microRNAs were down-regulated more than 1.5 times. Furthermore, we revealed 17 microRNAs which were changed at all three time points post-injury, the notable two microRNAs were miR-142-3p and miR-221, which could involve in many pathophysiological processes. Conclusions Our microarray-based bioinformatics analysis has showed that microRNAs in rat hippocampus have different expression levels at various time points post-injury, suggesting it is likely an active approach for cells to cope with injury through modulating microRNA expression, and microRNAs play key roles in the pathological courses after injury and work collaboratively like a complicated gene expression network. MiR-142-3p and miR-221 may be used as potentially biological markers for TBI assessment, and thus make some improvements in exploratory research for TBI-related cognitive disorder. | |||
TO cite this article:SUN Tingyi,LIU Zilong,LIU Liang, et al. Expression profiling of microRNAs in hippocampus of rats following traumatic brain injury[OL].[28 June 2013] http://en.paper.edu.cn/en_releasepaper/content/4549672 |
2. A new PCR- Ligase Detection Reaction (LDR) system for forensic SNP analysis | |||
weibo liang,lvmeili,liao miao,zhuo wang,yanyun wang,lin zhang | |||
Clinical Medicine 03 January 2008 | |||
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Abstract:Forensic DNA analysis is routinely performed using polymorphic short tandem repeat (STR) markers. Nevertheless, for degraded or minute DNA samples, analysis of autosomal single nucleotide polymorphisms (SNPs) in short fragments might be more successful. Furthermore Y-chromosomal SNPs analysis is a more useful tool in forensic investigations. Recently most methods of SNPs typing are based on the principle of minisequencing. Here we developed a new SNPs detection system for limited forensic materials based on the Ligase Detection Reaction (LDR) assay. A set of 8 Y-SNPs have been typed in 232 individuals of two different Chinese populations via multiplexed Ligase Detection Reaction assay through the Applied Biosystem 310 after the multiplexed PCR. Three probes for each Y-SNP were designed including one common fluorescence labeled probe and two allele specific probes different in size. All the amplicons are less than 100 bps for easy detection degraded or minute DNA samples. Even though the number of markers in the current system is limited, it can easily be extended to yield a greater power of discrimination. When fully developed, the PCR-LDR analysis provides a promising system for efficient sensitive SNP analysis of forensic samples in the future. | |||
TO cite this article:weibo liang,lvmeili,liao miao, et al. A new PCR- Ligase Detection Reaction (LDR) system for forensic SNP analysis[OL].[ 3 January 2008] http://en.paper.edu.cn/en_releasepaper/content/17712 |
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