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1. Simultaneous extraction of baicalin and saccharides from Scutellariae Radix with aqueous two-phase system | |||
Yuan Xiaolin,Mu Xiaojing,Li Yan,Gou Yan,Tang Meiling,Xiao Shangyou | |||
Traditional Chinese Medicine and Pharmacology 27 April 2020 | |||
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Abstract:Scutellariae Radix is a Chinese herb for detoxification. Efficient extraction of its main active components baicalin and saccharides is interesting to pharmaceutical and cosmetic fields. The baicalin and saccharides were efficiently extracted with aqueous two-phase systems (ATPSs) Ethanol-(NH4)2SO4-H2O (EtSO) and Ethanol-cosolvent-(NH4)2SO4-H2O (EtSO-cosolvent). The factors such as pH, (NH4)2SO4 concentration, ethanol fraction, cosolvent fraction, extraction time and temperature were optimized and thermodynamics for partition was discussed. With the extractions at 48 C for 40 min, the EtSO, EtSO-MtOH and EtSO-Acetone exhibited higher baicalin yield than conventional 70% ethanol system (v/v) by 21.4%, 24.8% and 26.2%, respectively; and they displayed saccharides yield in a similar level to that with water system. By dialysis method the saccharides extracted with ATPS was proved to have similar molecular weight to those extracted with water. Therefore, it is feasible to obtain two birds (two targets) with one stone (ATPS) in high efficiency. | |||
TO cite this article:Yuan Xiaolin,Mu Xiaojing,Li Yan, et al. Simultaneous extraction of baicalin and saccharides from Scutellariae Radix with aqueous two-phase system[OL].[27 April 2020] http://en.paper.edu.cn/en_releasepaper/content/4751820 |
2. The antioxidant and antimicrobial activities of aucubin and aucubigenin | |||
LI Yang,ZHAO Jiawei,ZHANG Zhen,ZHAO Ye | |||
Traditional Chinese Medicine and Pharmacology 16 May 2016 | |||
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Abstract:In this study, the iridoid glycoside aucubin from the seeds of Eucommia ulmoides Oliv and its aglycone aucubigenin have been determined for their in vitro antioxdant and antimicrobial activities. 2,2-diphenyl-2-picryhydrazyl (DPPH) radical, superoxide anion radical, and hydroxyl radical, were established to invested the antioxdant activities. The antimicrobial activities of aucubigenin and aucubin were also determined by the disc diffusion method. The results showed for the first time that the two compounds exhibited significant differences in antioxdant activities. While aucubin exhibited no antimicrobial activity, aucubigenin exhibited considerable antimicrobial activity against all of the tested bacteria. | |||
TO cite this article:LI Yang,ZHAO Jiawei,ZHANG Zhen, et al. The antioxidant and antimicrobial activities of aucubin and aucubigenin[OL].[16 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4691078 |
3. A sensitive standardized method for determining antimicrobial activity of iridoid glycoside geniposide and its aglycone | |||
LI Yang,ZHANG Zhen,ZHAO Jiawei,ZHAO Ye | |||
Traditional Chinese Medicine and Pharmacology 15 May 2016 | |||
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Abstract:The iridoid glycoside geniposide and its aglycone genipin have been investigated for their antimicrobial activities through micro-well dilution with automated spectrophotometric method, which was more accurate than paper disc diffusion method. The results showed geniposide exhibited no antimicrobial activity, while genipin showed remarkable antimicrobial activity against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella enterica. Genipin was particularly more effective against Staphylococcus aureus with MIC value of 62.5 ug/mL and MBC value of 125 ug/mL. | |||
TO cite this article:LI Yang,ZHANG Zhen,ZHAO Jiawei, et al. A sensitive standardized method for determining antimicrobial activity of iridoid glycoside geniposide and its aglycone[OL].[15 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4690868 |
4. Preparation, hydrolysis and bioactivity evaluation of a natural polysaccharide from sclerotium of Poria cocos | |||
Chen Qi,Yang Jia,Xu Wenjin | |||
Traditional Chinese Medicine and Pharmacology 08 March 2016 | |||
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Abstract:Pachyman isolated from a natural polysaccharide is lack of bioactivity due to its water-insolubility. In this study, in order to improve its bioactivity, pachyman was enzymatically hydrolyzed to obtain water soluble pachyman (WSP). Moreover, in order to further evaluate the relationship between the structure and bioactivity of pachyman, carboxymethylated pachyman (CMP) with different degree of substitution (DS) and water-soluble pachyman with different molecular weight were also prepared and their antitumor activities against S180 cells were further evaluated. Our results showed that both enzymatic hydrolysis and chemical modification were effective for improving the antitumor activity of pachyman. However, our data also suggested that the molecular weight and the degree of substitution of the derivatives should be taken into account when developing polysaccharide-based drugs. | |||
TO cite this article:Chen Qi,Yang Jia,Xu Wenjin. Preparation, hydrolysis and bioactivity evaluation of a natural polysaccharide from sclerotium of Poria cocos[OL].[ 8 March 2016] http://en.paper.edu.cn/en_releasepaper/content/4679252 |
5. Reduning Injection Sensitization in Guinea pigs | |||
Wang Fang,Peng Guoping,Xiao Wei,Wang Zhenzhong,Zheng yunfeng,Li Cunyu,Zhu Huaxu | |||
Traditional Chinese Medicine and Pharmacology 25 July 2014 | |||
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Abstract:Reduning injection, a traditional Chinese medicine injection, has multiple functions such as clearing heat, dispelling wind, as well as detoxification. However, there are a few reports of severe anaphylaxis during the clinical application of reduning. The aim of this study is to determine the sensitization to reduning in guinea pigs and the underlying cause of the anaphylactic reaction. The compositions and Tween-80 in reduning were determined before and after ultrafiltration. Egg albumin, ultrafiltered reduning, unfiltered reduning, Tween-80 and nine compositions in reduning were selected to sensitize and stimulate the animals. Changes in the levels of plasma 5-hydroxytryptamine were used to analyze the effect of ultrafiltration on the sensitization effect of reduning injection. We detected a significant decrease in Tween-80 content but a less significant decrease in the other components of the injection following ultrafiltration. Unfiltered reduning injection, Tween-80, and chlorogenic acid caused remarkable sensitization on guinea pigs while the filtered reduning injection resulted in a significantly lower degree of sensitization. These results suggest that ultrafiltration significantly reduced the sensitizing effect of reduning injection, which is likely due to Tween-80 content. Additionally, the form of chlorogenic acid within the complex solution mixture may also affect the sensitizing effect of chlorogenic acid. | |||
TO cite this article:Wang Fang,Peng Guoping,Xiao Wei, et al. Reduning Injection Sensitization in Guinea pigs[OL].[25 July 2014] http://en.paper.edu.cn/en_releasepaper/content/4604851 |
6. Antitumor activity and toxicity relationship of annonaceous acetogenins in mice | |||
CHEN Yong,CHEN Jianwei,WANG Yu,XU Shasha,WANG Siliang,LI Xiang | |||
Traditional Chinese Medicine and Pharmacology 21 May 2012 | |||
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Abstract:Annonaceous acetogenins (ACGs) are one of the most interesting classes of natural products appearing in the past two decades. Here, we studied the antitumor activity and toxicity relationship of ACGs covering the three major structural classes of mono-tetrahydrofuran (THF), adjacent bis-THF and nonadjacent bis-THF compounds including annosquamin B (1), bullatacin (2) and Annosquatin B (3) in vivo. A single intraperitoneal (i.p.) injections of 100 μg/kg of 1-3 did not cause side effects in normal mice. Five doses of 25 and 50 μg/kg of 2 to H22 hepatoma cells xenografts bearing mice resulted in a above 61% reduction in tumor growth with no pathological changes, but hematologic parameters increased significantly in normal mice. Ten doses of 25, 50 and 100 μg/kg of 1 and 3 to the H22 hepatoma cells transplantation tumor model mice resulted in a maximum 53.7% and 58.7% reduction in tumor growth, respectively, and did not cause severe side effects in normal mice. This study provided the evidence that adjacent bis-THF ACGs showed higher antitumor activity, and also exhibited higher toxicity than mono-THF and nonadjacent bis-THF ACGs in vivo. | |||
TO cite this article:CHEN Yong,CHEN Jianwei,WANG Yu, et al. Antitumor activity and toxicity relationship of annonaceous acetogenins in mice[OL].[21 May 2012] http://en.paper.edu.cn/en_releasepaper/content/4479256 |
7. Six cytoxic annonaceous acetogenins from Annona squamosa seeds | |||
CHEN Yong,CHEN Jianwei,WANG Yu,XU Shasha,LI Xiang | |||
Traditional Chinese Medicine and Pharmacology 16 May 2012 | |||
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Abstract:Custard apple (Annona squamosa L.) is an edible tropical fruit, and its seeds had been used in south China as folk medicine to treat "malignant sore" (cancer) and other usage as insecticide. Phytochemical investigation of the ethanol fraction of custard apple seeds led to the isolation of six new annonaceous acetogenins, annosquacins A-D (1-4), annosquatin A (5) and annosquatin B (6). Their structures were elucidated by spectroscopic analysis. Compounds 1-4 are adjacent bistetrahydrofuran annonaceous acetogenins. Compounds 5 and 6 are non-adjacent bistetrahydrofuran annonaceous acetogenins and the first examples in which the tetrahydrofuran ring system is located between C-9 and C-20. The absolute configurations of 1-6 were defined by the application of the Mosher method. Compounds 1-6 exhibited potent cytotoxic activity in vitro against five human tumor cell lines. 5 and 6 showed a high selectivity toward the MCF-7 and A-549 cell line respectively. | |||
TO cite this article:CHEN Yong,CHEN Jianwei,WANG Yu, et al. Six cytoxic annonaceous acetogenins from Annona squamosa seeds[OL].[16 May 2012] http://en.paper.edu.cn/en_releasepaper/content/4478772 |
8. squamosa seeds extract containing annonaceous acetogenin compounds | |||
CHEN Yong,XU Shasha,CHEN Jianwei,WANG Yu,XU Huiqing,FAN Naibing,LI Xiang | |||
Traditional Chinese Medicine and Pharmacology 15 May 2012 | |||
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Abstract:Seeds of Annona squamosa L. have been used in the south of China as a folk remedy to treat "malignant sores" (cancer). To investigate the chemical constituents and the anti-tumor activity of the standardized A. squamosa seeds extract in vitro and in vivo. Annonaceous acetogenin profiles of the standardized extract were determined by using Fourier transform infrared (FT-IR) and high performance liquid chromatography (HPLC) techniques. The anti-tumor activity of the extract was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) cytotoxicity in vitro and H22 hepatoma cells transplantation tumor model in vivo. The FT-IR spectroscopy showed the presence of annonaceous acetogenin compounds in the extract. Two major annonaceous acetogenins: 12, 15-cis-squamostatin-A and bullatacin were identified and quantified by HPLC. The seed extract showed significant anti-tumor activity against four human tumor cell lines, especially for MCF-7 (IC50. 0.25 μg/ml) and Hep G2 (IC50. 0.36 μg/ml) cells in vitro. The extract inhibited the growth of H22 tumor cells in mice with a maximum inhibitory rate of 69.55% by oral administration. A. squamosa seed extract showed significant anti-tumor activities against human hepatoma cells in vitro and in vivo, indicating a potential for developing the extract as a novel anti-liver cancer drug. | |||
TO cite this article:CHEN Yong,XU Shasha,CHEN Jianwei, et al. squamosa seeds extract containing annonaceous acetogenin compounds[OL].[15 May 2012] http://en.paper.edu.cn/en_releasepaper/content/4478623 |
9. Monodisperse core-shell microstructured molecular imprinting silica for highly selective enrichment of isoflavonoid glycosides from Radix Puerariae | |||
Jing Luo,Qing Zhang,Liying Zhang,Jiawei Zhao,Shuhu Du | |||
Traditional Chinese Medicine and Pharmacology 20 April 2012 | |||
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Abstract:This paper reports the preparation of puerarin (PR) imprinted layer-coated silica microparticles toward selective recognition of PR and fast affinity-enrichment of the main isoflavonoid glycosides from the crude extract of Radix puerariae. Before the preparation, quantum mechanics (QM) method was applied to identify three kinds of common functional monomers capable of interaction with PR and then predict optimal functional monomer (acrylamide, AA) and the relative molar ratio of template to functional monomer (PR/AA, 1 : 4). The obtained PR-imprinted silica microparticles were evaluated by transmission electron microscope (TEM) and rebinding experiments, exhibiting good morphology and high binding affinity to PR. Meanwhile, the rebinding amount of the imprinted microparticles to PR was nearly 2.1-folds that of non-imprinted microparticles. When the PR-imprinted microspheres were used as packing materials for solid-phase extraction, the recovery yields of PR, daidzin (DD) and genistin (GS) were simultaneously up to 90% by one-step extraction from the crude extract of Radix puerariae. Additionally, the PR-imprinted microparticles could be re-used for at least 5 times without losing any extraction efficiency. These results indicate that the PR-imprinted microparticles have highly selective adsorption capabilities to PR, DD and GS from the crude extract of Radix puerariae. The method of molecularly imprinted polymers (MIPs) coupled with solid-phase extraction (SPE) provides a good solution of the enrichment and separation of active extracts from complicated traditional Chinese medicine (TCM) with certain structures. | |||
TO cite this article:Jing Luo,Qing Zhang,Liying Zhang, et al. Monodisperse core-shell microstructured molecular imprinting silica for highly selective enrichment of isoflavonoid glycosides from Radix Puerariae[OL].[20 April 2012] http://en.paper.edu.cn/en_releasepaper/content/4476028 |
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