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1. QCM biosensor for detection of small nucleic acid fragments: sandwich reaction and signal amplification by silver nanoclusters | |||
GUO Jia,JIANG Qifa,Rui-Qin Fang | |||
Chemistry 24 September 2021 | |||
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Abstract:This article presents a quartz crystal microbalance (QCM) biosensor for the determination of nucleic acids by modifying electrodes with cysteine (Cys), polyethylene glycol (PEG), and silver nanoclusters (AgNCs), which represents a new approach with increased specificity and sensitivity of detection. The probe deoxyribonucleic acid immobilized on the surface of the QCM gold electrode hybridizes with a cysteine conjugate to form a specific recognition layer. The unmodified sites are then non-specifically closed by polyethylene glycol. The target gene is subsequently captured by the immobilized probe, resulting in a change in frequency. The addition of silver ions with reducing agents in an ammonia environment to form silver nanoclusters of DNA-AgNCS was used to further extend the sensitivity of the QCM sensor. The results show that it is possible to expand the frequency variation of the QCM sensor by a factor of 63.6 using such a method. A linear relationship between frequency change and DNA concentration was observed in the range of 1 nM to 10 M of target gene concentration. Thus, this biosensor provides a low-cost and sensitive tool for detecting DNA. | |||
TO cite this article:GUO Jia,JIANG Qifa,Rui-Qin Fang. QCM biosensor for detection of small nucleic acid fragments: sandwich reaction and signal amplification by silver nanoclusters[OL].[24 September 2021] http://en.paper.edu.cn/en_releasepaper/content/4755569 |
2. Simultaneous determination of concentration and enantiomeric excess of amino acids with a coumarin-derived achiral probe | |||
YANG Lamei,WEI Weili | |||
Chemistry 24 March 2021 | |||
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Abstract:The chirality of amino acids plays important role in biological and medical sciences. The development of achiral small-molecule probes that can simultaneously determine the absolute configuration, enantiomeric excess, and total concentration of amino acids is significant. The current available achiral coumarin aldehyde probe that could form Schiff bases with free amino acids at room temperature to induce the CD signals and to change UV-vis signals. The red-shifted UV-vis signals were independent of the substrate\'s chirality and could be used to determine the total concentration. Conversely, the enantioselective CD signals could be used to determine the absolute configuration and enantiomeric excess. The usefulness and practicability of this sensing method were demonstrated by analyzing 6 non-racemic phenylalanine samples with different enantiomeric compositions and concentrations. | |||
TO cite this article:YANG Lamei,WEI Weili. Simultaneous determination of concentration and enantiomeric excess of amino acids with a coumarin-derived achiral probe[OL].[24 March 2021] http://en.paper.edu.cn/en_releasepaper/content/4754323 |
3. Aptamer-conjugated Nanomaterials for Biological Targets Detection | |||
ZHANG Ge,PENG Cheng,HU Xiaoxiao | |||
Chemistry 28 May 2018 | |||
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Abstract:Aptamers offer unique advantages in terms of wide range of targets, high affinity and specificity, and have been widely used in many fields such as basic research, disease diagnosis, and drug development. Nanomaterials, due to their nanoscale sizes, shapes and structures, exhibit a variety of features such as large specific surface area and new properties of optical, magnetic, thermal and electrical performance. Hence, aptamer-conjugated nanomaterials composed of aptamers and nanomaterials combine both benefits, enabling high-specificity detection of biological targets in complex environments. This review summarizes the characteristics of various highly efficient aptamer-conjugated nanomaterials detection platforms and their advantages in detection of biotargets in recent years, and demonstrates their potential for biological detection and disease diagnosis. | |||
TO cite this article:ZHANG Ge,PENG Cheng,HU Xiaoxiao. Aptamer-conjugated Nanomaterials for Biological Targets Detection[OL].[28 May 2018] http://en.paper.edu.cn/en_releasepaper/content/4745273 |
4. An Electrochemical Sensor for Prothrombin-Time Test | |||
XIN Lifei,LIU Hong | |||
Chemistry 22 March 2018 | |||
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Abstract:As a new interdisciplinary, blood coagulation and thrombosis, being involved in the development and progression of many diseases, have a prominent clinical significance. Prothrombin time (PT time) is the time when calcium and tissue factor is added into the blood sample until when the blood sample completely clots. Clinically, PT time is an extremely sensitive screening index in coagulation system, it has an irreplaceable influence to patients\' daily medication and related treatments as it can primarily reflect the normality of the extrinsic coagulation. Until recently, most automated coagulation analyzers used in hospitals can detect a variety of samples and projects at the same time with high accuracy. However it could only be operated by the professionals, not to mention the high production and testing costs. For these reasons, the frequency and ease-of-operation of the detection have been limited. In order to develop a real-time and household detection method, paper-chips and POCT technology are mainly utilized in this work. Paper-chips possesses their own advantages, as it can easily reflect the blood clotting process by detecting a change in the conductance of blood during the blood coagulation process. After a series of design, tests and improvements, a chip for PT time testing is fabricated. Using this chip, the PT time of several blood samples are tested, and the testing results confirmed that the further improved chip can complete the measurement and correction of blood PT time test. Compared with conventional coagulation analyzer, paper-chips have several intrinsic advantages such as reduced cost, higher accuracy, and better application in POCT use. We believe that pushing the further experimental products to market could fill the vacancy in the domestic industry. | |||
TO cite this article:XIN Lifei,LIU Hong. An Electrochemical Sensor for Prothrombin-Time Test[OL].[22 March 2018] http://en.paper.edu.cn/en_releasepaper/content/4744019 |
5. Patterned Photonic Nitrocellulose Membrane for Bio-detection Based on Coffee-Ring Effect | |||
Litianyi Tang,Hong Liu | |||
Chemistry 19 March 2018 | |||
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Abstract:In this work, we report a method for the fabrication of nitrocellulose (NC) membrane with photonic crystal (PC) pattern for bio-detection. The membrane is prepared by imprinting the corresponding PC pattern into it through thermal Nano-imprint method. Aptamer beacon marked with fluorescein is used for detection of target molecules. Only one droplet (2-3μl) containing the aptamer-target complexes is dripped onto the nitrocellulose membrane. After evaporation (15-20min), the droplet is completely dried and the complexes form a coffee ring on the membrane. The ring keep growing with the increase of complexes concentration .As the aptamer beacon is marked with fluorescein and PC pattern has a fluorescence enhancement effect, the fluorescent signal on the coffee ring for detection is considerable improved. Fluorescent detection of adenosine triphosphate (ATP) is used for testing the application of this method. Owing to fluorescence enhancement effect of PC, the fluorescent signal for detection is improved and the limit of detection is 80.2μM for ATP. Therefore, we believe this method is promising for the fabrication of patterned photonic nitrocellulose membrane for bio-detection and point-of-care testing. | |||
TO cite this article:Litianyi Tang,Hong Liu. Patterned Photonic Nitrocellulose Membrane for Bio-detection Based on Coffee-Ring Effect[OL].[19 March 2018] http://en.paper.edu.cn/en_releasepaper/content/4743982 |
6. Aptamer-modified Semiconductor Quantum Dots for Biosensing Applications | |||
WEN Lin,ZHANG xiaobing | |||
Chemistry 07 June 2017 | |||
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Abstract:Semiconductor quantum dots have attracted growing interest in biosensing area, because of their unique properties, such as narrow and symmetric emission with tunable colors, high quantum yield, high stability and controllable morphology. The introduction of various reactive functional groups on the surface of semiconductor quantum dots allows us to conjugate a spectrum of ligands, antibodies, peptides, or nucleic acids for broader and smarter applications. Among these ligands, aptamers exhibit many advantages including small size, high chemical stability, simple synthesis with high batch-to-batch consistence and convenient modification. More importantly, it is facile to introduce nucleic acid amplification strategies and/or nanomaterials to improve the sensitivity of aptamer-based sensing systems. Therefore, the combination of semiconductor quantum dots and aptamers brings more opportunities in bioanalysis. In this review, we summarize recent advances of aptamer-functionalized semiconductor quantum dots in biosensing applications. Firstly, we discuss the structure and properties of semiconductor quantum dots, as well as aptamers. Then, the application of biosensors based on aptamer-modified semiconductor quantum dots is discussed in the context of different signal transducing mechanisms, including optical, electrochemical and electrogenerated chemiluminescence approaches. Finally, we provide our perspectives on the current challenges and opportunities in this promising field. | |||
TO cite this article:WEN Lin,ZHANG xiaobing. Aptamer-modified Semiconductor Quantum Dots for Biosensing Applications[OL].[ 7 June 2017] http://en.paper.edu.cn/en_releasepaper/content/4737088 |
7. A colorimetric and fluorescent probe for rapid determination of copper in water and living cells | |||
Nie Hailiang,Zhang Xiaoling | |||
Chemistry 27 April 2017 | |||
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Abstract:Detection of copper ions (Cu2+) is of great significance in protecting the environment and human health. In the present work, we developed a new colorimetric and fluorescent probe for Cu2+ determination with high selectivity and rapid-response rate. Upon Cu2+ addition, the probe underwent an 86 nm blue-shift in absorption peak wavelength as well as a significant quenching in fluorescence emission. The fluorescence intensity at 575 nm showed a good linear relationship (R2 = 0.999) with the Cu2+ concentration in the range of 0 μM ~ 12 μM. Using the probe as an analytical tool, the detection of Cu2 + can be done within 5 s, whose process was not subject to interference from other metal ions and pH changes. Moreover, the probe can be used for Cu2+ assay in both real water sample and living cells. | |||
TO cite this article:Nie Hailiang,Zhang Xiaoling. A colorimetric and fluorescent probe for rapid determination of copper in water and living cells[OL].[27 April 2017] http://en.paper.edu.cn/en_releasepaper/content/4728954 |
8. Self-protection Electrochemical Sensor Based on Temperature-responsive Polymer | |||
Zhao Pengcheng,Chen Chao,Xie Yixi,Li Chunyan,Fei Junjie | |||
Chemistry 26 April 2017 | |||
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Abstract:This work reports the application of a novel thermo-sensitive polymer PS-PNIPAm-PS in electrochemistry. It was fabricated and modi?ed onto a working electrode to form a self-protection electrochemical sensor. After testing, at low temperatures, it didn't affect the normal measurement of the working electrode; but at high temperatures, it can automatically shrink to form a dense film, to prevent electron transfer, protect the electrode surface is not damaged, increase its service life.This research provides a novel method for electrode protection, high temperature protection of batteries and new sensors production. | |||
TO cite this article:Zhao Pengcheng,Chen Chao,Xie Yixi, et al. Self-protection Electrochemical Sensor Based on Temperature-responsive Polymer[OL].[26 April 2017] http://en.paper.edu.cn/en_releasepaper/content/4729697 |
9. A naphthalimide based fluorescent probe for imaging microviscosity in living cells | |||
Guo Bingpeng,Zhang Xiaoling | |||
Chemistry 24 April 2017 | |||
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Abstract:Intracellular viscosity strongly influences transportation of mass and signal, interactions between the biomacromolecules and diffusion of reactive metabolites in live cells. Herein, we developed a naphthalimide-hemicyanine based fluorescent probe Cyt-NA for imaging the change of microviscosity in living cells. Cyt-NA exhibited about 260 folds fluorescence increasing at 610 nm along with the increasing viscosity from 1.0 cP to 1410 cP. These satisfying response properties make Cyt-NA possible to monitor viscosity changes under starvation and low temperature conditions, offering us more practical information about microenviromental viscosity in living cells. | |||
TO cite this article:Guo Bingpeng,Zhang Xiaoling. A naphthalimide based fluorescent probe for imaging microviscosity in living cells[OL].[24 April 2017] http://en.paper.edu.cn/en_releasepaper/content/4728806 |
10. Raman Spectroscopy a Promising Technique for Quantification and Monitoring Heat-induced Formation of Trans Fatty Acids in Oil | |||
SHI Ruyi,LIU Xiangjiang,XIE Lijuan,YING Yibin | |||
Chemistry 18 April 2017 | |||
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Abstract:Trans fatty acids (TFAs), a type of unsaturated fatty acids that have been widely used in the food industry, have drawn considerable attention recently since consumption of TFAs significantly increased the risk of coronary heart disease, diabetes, etc. Thus, efficient and reliable detection methods for TFAs are of great importance both in industrial applications and for research purposes. In this paper, we developed a rapid and pretreatment-free quantification approach for TFA in oils using Raman spectroscopy together with some chemometric methods. The results show that the concentration of TFA in various oils can be well predicted based on the Raman spectral features in the region of 1640 - 1680 cm-1 (characteristic to C=C stretching modes), yielding a root mean square error of prediction (RMSEP) equal to 0.47%. Furthermore, in order to understand heat-induced formation of TFA during food preparation, we investigated the TFA's concentrations of the oil heated under different temperatures based on the above predication model. The result indicates its concentration increased sharply when the heating temperature exceeded 169 ℃. All these results suggest that Raman spectroscopy is a promising tool for quantification of TFAs, offering the benefit of avoiding time-consuming, costly, sample pretreatments and laborious chemical analysis. | |||
TO cite this article:SHI Ruyi,LIU Xiangjiang,XIE Lijuan, et al. Raman Spectroscopy a Promising Technique for Quantification and Monitoring Heat-induced Formation of Trans Fatty Acids in Oil[OL].[18 April 2017] http://en.paper.edu.cn/en_releasepaper/content/4725585 |
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