Authentication email has already been sent, please check your email box: and activate it as soon as possible.
You can login to My Profile and manage your email alerts.
If you haven’t received the email, please:
|
|
There are 1 papers published in subject: > since this site started. |
Results per page: |
Select Subject |
Select/Unselect all | For Selected Papers |
Saved Papers
Please enter a name for this paper to be shown in your personalized Saved Papers list
|
1. Solubilization and refolding with simultaneous Purification of Recombinant Human Granulocyte Colony-stimulating Factor expressed by Escherichia coli as Inclusion Bodies | |||
Wang Chaozhan ,Wang Lili ,Geng Xindu | |||
Chemistry 06 January 2008 | |||
Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
Abstract:Recombinant human granulocyte colony-stimulating factor (rhG-CSF) was solubilized and purified from inclusion bodies expressed in Escherichia coli. Effect of both pH and urea on the solubilization of rhG-CSF inclusion bodies were investigated, the results indicate that the existence both ionic and hydrophobic interactions in the inclusion bodies. Various solvents with reduced/oxidized glutathione (GSH/GSSG) were used to solubilize rhG-CSF inclusion bodies,the extent of solubilization was compared with each other and the behaviors of rhG-CSF in high performance hydrophobic interaction chromatography (HPHIC) were investigated. Solubilization of rhG-CSF inclusion bodies by 2% Sarkosyl in the presence of GSH/GSSG probably helps oxidizing rhG-CSF to form disulfide bonds, but Sarkosyl in the solution interferes seriously with the chromatographic process. To solubilize rhG-CSF inclusion bodies by combination of 2% Sarkosyl and 8.0 molL-1 urea can overcome this problem very well. And the so solubilized rhG-CSF was simultaneously refolded and purified by one step of HPHIC, with a specific activity of 1.9 × 108 IUmg-1 and a purity of 97%, the mass recovery was 41%. | |||
TO cite this article:Wang Chaozhan ,Wang Lili ,Geng Xindu . Solubilization and refolding with simultaneous Purification of Recombinant Human Granulocyte Colony-stimulating Factor expressed by Escherichia coli as Inclusion Bodies[OL].[ 6 January 2008] http://en.paper.edu.cn/en_releasepaper/content/17777 |
Select/Unselect all | For Selected Papers |
Saved Papers
Please enter a name for this paper to be shown in your personalized Saved Papers list
|
Results per page: |
About Sciencepaper Online | Privacy Policy | Terms & Conditions | Contact Us
© 2003-2012 Sciencepaper Online. unless otherwise stated