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Expression Analysis of Nine Heat Shock Protein Genes in Rice
Jie Zou 1,Ailing Liu 2,Xinbo Chen 3 *,Xiaoyun Zhou 3,Guofu Gao 2,Wenfang Wang 2,Xianwen Zhang 2
1.Crop Gene Engineering Key Laboratory of Hunan Province, College of Bioscience and Biotechnology,Hunan Agricultural University
2.College of Bioscience and Biotechnology,Hunan Agricultural University
3.Crop Gene Engineering Key Laboratory of Hunan Province, Hunan Agricultural University
*Correspondence author
#Submitted by
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Funding: 国家重点基础研究发展计划,高等学校博士学科点专项科研基金资助课题,湖南省自然科学基金资助项目(No.2007CB116207,20050537001,05JJ30035)
Opened online:16 April 2008
Accepted by: none
Citation: Jie Zou,Ailing Liu,Xinbo Chen.Expression Analysis of Nine Heat Shock Protein Genes in Rice[OL]. [16 April 2008] http://en.paper.edu.cn/en_releasepaper/content/20532
 
 
Heat shock proteins (HSPs) have been found to be ubiquitous. More than 70 putative HSP genes were found in rice (Oryza sativa L.), but few of them have been characterized. Expression patterns of nine rice OsHSP genes were analyzed by semi-quantitative RT-PCR. The nine OsHSPs were named OsHSP80.2, OsHSP74.8, OsHSP71.1, OsHSP58.7, OsHSP50.2, OsHSP26.7, OsHSP24.3, OsHSP24.1 and OsHSP16.9 respectively according to their approximate molecular weight. The nine genes showed different expression profiles in roots, leaves, leaf sheaths, spikes and embryos. All nine OsHSP genes were induced strongly by heat shock treatment, whereas none of them were induced by cold. The transcripts of OsHSP80.2, OsHSP71.1 and OsHSP24.3 were increased during salt tress treatment. Expression of OsHSP80.2 and OsHSP24.1 genes were enhanced while treated with 10% PEG. Only OsHSP71.1 was induced by ABA while OsHSP24.1 was inhibited by ABA.
Keywords:heat shock proteins (HSPs), expression patterns, rice, semi-quantitative RT-PCR
 
 
 

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