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Isolation and Characterization of Homolog PtLEAFY promoter in Trifoliate Orange (Poncirus trifoliata L. Raf. )
Mei Li,Zhang Jinzhi,Li Zhimin,Hu Chungen *
College of Horticulture and Forestry Science,Huazhong Agricultural University, Wuhan, P. R. China 430070
*Correspondence author
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Funding: 教育部博士点基金(No.2005050416)
Opened online:19 January 2009
Accepted by: none
Citation: Mei Li,Zhang Jinzhi,Li Zhimin.Isolation and Characterization of Homolog PtLEAFY promoter in Trifoliate Orange (Poncirus trifoliata L. Raf. )[OL]. [19 January 2009] http://en.paper.edu.cn/en_releasepaper/content/28042
 
 
Abstract The long juvenile phase for 6 to 8 years in woody plants has become a serious obstacle for genetic analysis and breeding practice. In order to understand the flower transition mechanism in woody plants, DNA sequence of a LEAFY homologue in trifoliate orange (Poncirus trifoliata L. Raf.) was isolated and characterized. A chimeric expression construct PtLEAFY::GUS indicated the promoter from woody plant acted its role in Arabidopsis thiliana from 7-day old shoot apical meristem to rosette leaves. These findings concluded that PtLEAFY promoter from woody plants had immediate function in annual plants and may potential provide the specific promoter of shoot apical meristem .
Keywords:LEAFY;Promoter;Transgenic Arabidopsis
 
 
 

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