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Cross-species microsatellites amplification for a vulnerable species endemic of China, Reeves’s pheasant (Syrmaticus reevesii)
Wang Ning 1,Chang Jiang 1,Sun Quanhui 2,Gu Langyu 1,Zhangzhengwang 1 * #
1.MOE Key Laboratory for Biodiversity Sciences and Ecological Engineering, College of Life Sciences Beijing Normal University
2.Beijing Raptor Rescue Center
*Correspondence author
#Submitted by
Subject:
Funding: 高等学校博士学科点专项科研基金 ,国家自然科学基金(No.20050027021,30570234)
Opened online:25 February 2009
Accepted by: none
Citation: Wang Ning,Chang Jiang,Sun Quanhui.Cross-species microsatellites amplification for a vulnerable species endemic of China, Reeves’s pheasant (Syrmaticus reevesii) [OL]. [25 February 2009] http://en.paper.edu.cn/en_releasepaper/content/29630
 
 
Cross-species microsatellite amplification can be an effective way in obtaining microsatellite loci for closely related taxa in bird species. The Reeves’s pheasant, Syrmaticus reevesii, is a vulnerable species endemic of China. In order to study the population genetics and paternity of its wild populations, we obtained 10 polymorphic microsatellite markers from cross-species amplification. The number of alleles per locus varies between two and twelve, with expected heterozygosity ranging from 0.32 to 0.802 (n =75). These polymorphic loci are facilitating the study of paternity identification of Reeves’s pheasant and also provide a valuable tool for future studies on conservation genetics of this species.
Keywords:Syrmaticus reevesii;microsatellite;primers;cross-species amplification
 
 
 

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