|
Soxhlet extraction of hot pepper through an orthogonal experiment (L9(34)) with both recovery of capsaicinoids and time interval as indexes were investigated to get the best extraction conditions. Integrated Balance Method was used to select the condition for conformation experiment, and two groups of conditions were chosen : A3B3C2D1 and A3B3C2D3,number as 1 and 2 respectively. Result showed that experiment 2 has a little lower recovery of capsaicinoids, but it used a shorter time. In order to improve recovery of capsaicinoids of experiment 2, extraction cycle was increased to 20 times. The recovery of capsaicinoids is close to experiment 1. The total time used is 140 min which is shorter than experiment 1. Finally, the best conditions were selected: extraction cycle, extraction temperature, volume of solvent(95%ethanol) and weight of hot pepper were 20, 140℃and 130 mL and 20g respectively. It showed that Soxhlet extraction was an efficient, economical, and easy method for extraction of capsaicinoids. |
|
Keywords:Soxhlet extraction;orthogonal experiment;capsaicinoids;Integrated Balance Method |
|