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Dynamic urinary proteomic analysis
Sun Wei 1 * #,Chen Yong 2,Gao Youhe 1
1.Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences
2.Lanzhou Institute of Biological Products The China National Biotech Group,Lanzhou University
*Correspondence author
#Submitted by
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Funding: 高等学校博士点新教师专项科研基金(No.20070023071)
Opened online:24 March 2009
Accepted by: none
Citation: Sun Wei ,Chen Yong ,Gao Youhe .Dynamic urinary proteomic analysis [OL]. [24 March 2009] http://en.paper.edu.cn/en_releasepaper/content/30668
 
 
Human urinary proteome analysis is a convenient and efficient approach for understanding disease processes affecting the kidney and urogenital tract. Many potential biomarkers have been identified in previous differential analyses; however, dynamic variations of the urinary proteome have not been intensively studied, and it is difficult to conclude that potential biomarkers are genuinely associated with disease rather then simply being physiological proteome variations. In this paper, pooled and individual urine samples were used to analyze dynamic variations in the urinary proteome. Five types of pooled samples (first morning void, second morning void, excessive water-drinking void, random void, and 24 h void) collected in 1 day from six volunteers were used to analyze intra-day variations. Six pairs of first morning voids collected a week apart were used to study inter-day, inter-individual, and inter-gender variations. The intra-day, inter-day, inter-individual, and inter-gender variation analyses showed that many proteins were constantly present with relatively stable abundances, and some of these had earlier been reported as potential disease biomarkers. In terms of sensitivity, the main components of the five intra-day urinary proteomes were similar. The advantages and disadvantages of pooling samples are also discussed.
Keywords:urinary proteome;dynamic analysis;biomarker
 
 
 

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