Genetic diversity of “Candidatus Liberibacter asiaticus” isolates from different geographical regions in China

Zhou Binbin; Yin Youping; Hu Hao; Wang Zhongkang

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Genetic diversity of “Candidatus Liberibacter asiaticus” isolates from different geographical regions in China

Zhou Binbin #,Yin Youping,Hu Hao,Wang Zhongkang *

Genetic Engineering Research Center,College of Bioengineering, Chongqing University

*Correspondence author

#Submitted by

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Funding: 国家科技支撑计划重点项目,校企合作项目（No.2007BAD47B03-5,）

Opened online:26 May 2009

Accepted by: none

Citation: Zhou Binbin,Yin Youping,Hu Hao.Genetic diversity of “Candidatus Liberibacter asiaticus” isolates from different geographical regions in China[OL]. [26 May 2009] http://en.paper.edu.cn/en_releasepaper/content/32562

Citrus Huanglongbing (HLB) is one of the most destructive diseases. HLB was demonstrated grafting transmissibility via infected citrus scion in Southern China, therefore, it is important to investigate HLB disease epidemiology. In this study, a genetic diversity study was conducted using PCR-RFLP based on the outer membrane protein gene (omp) sequences. The amplicons of “Ca. L. asiaticus” were digested with enzymes. The difference of restriction patterns show that “Ca. L. asiaticus” may comprise several different omp strains, even among strains within a given region. DNA sequences and the deduced amino acid sequences of omp gene were compared to those in GenBank database with BLAST and the results were used to construct phylogenetic tree. The omp gene of “Ca. L. asiaticus” from China were highly homogeneous, but other “Candidatus. Liberibacter” species were not introduced or developed in china so far. The result is important on explorer HLB disease epidemiology and can help the scientific community develop more efficient methods to control the diseases.

Keywords:Huanglongbing;Candidatus Liberibacter asiaticus;RFLP;Genetic diversity;Phylogenetic analysis

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