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| eeds of Triticum aestivum L. cv. Zhengyin No1 were treated by 10 μmol/L GA3 and 10 μmol /L ABA before germination respectively. The growth of root and shoot, contents of endosperm amylose and soluble carbohydrate, medial pH value, the utilizing efficiency of storage materials (MUE), and activity of α-amylase in endosperm during seed germination under osmotic stress were investigated. The results showed that shoot growth was promoted by GA3 and inhibited by ABA and osmotic stress. Root growth was promoted by GA3, and retarded by osmotic stress and ABA at early stage. But the growth of root of seedlings from seeds treated by ABA was promoted significantly during the late germination stage. Seedling growth was positively correlated with medial pH and MUE significantly. GA3 promoted the expression and increase in activity of α-amylase in endosperm of wheat seed, resulting in increase in breakdown of amylose to soluble carbohydrates, but ABA and osmotic stress had the opposite effect to GA |
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| Keywords:α-amylase, GA3，ABA，Pretreatment, osmotic stress, seed，wheat |
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