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| To study the effects of low temperature on high-yield hybrid rice (Oryza sativa L.) at seedling stage, chlorophyll content, membrane lipid peroxidation, fatty acids composition and chloroplast ultrastructure of two varieties were investigated—a newly-developed cultivated super-high-yield hybrid rice LYPJ, and a traditional hybrid rice SY-63, setting 25℃ as control and 10℃ as low temperature treatment. In SY-63, chlorophyll content in rice leaves was decreased obviously with the duration at low temperature, whereas it did not decrease statistically in LYPJ. MDA (malondialdehyde) content increased slightly in LYPJ, but in SY-63, MDA content increased higher than those in 25℃-treated seedlings. The contents of saturated fatty acids decreased and the contents of unsaturated fatty acids increased in both of the two hybrids compared to the control. The IUFA (index of unsaturated fatty acid) of fatty acids increased in the two cultivars, being lower in LYPJ. The damage to the ultrastructure of chloroplasts was not so serious with increasing cold stress, but the number of osmiophilic granules in SY-63 increased significantly. The results indicated that LYPJ could have stronger chilling-resistance than SY-63 at seedling stage. |
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| Keywords:Hybrid rice (Oryza sativa L.);Low temperature;Membrane lipid peroxidation |
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