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Background Resent investigations implicated that autoantibody underlie some intractable seizure, such an anit-Munc18 (syntaxin-interacting protein) antibody. However, its mechanism is poorly understood.
Methods Anit-Munc-18 antibody was microinjected into CA1 area of hippocampus in Sprague-Dawley (SD) rat every other day for five times, followed up that every other week for additional five times. During experimental period, both electroencephalogram(EEG)and epileptiform activity (including spontaneous seizure) were monitored.
Results In twelve rats managed with antibody, ten rats (10/12)showed epileptiform EEG and nine rats(9/12)displayed epileptiform activity. Furthermore, 50 %( 5/10) eleptiform EEG and 44% (4/9) eleptiform activity had been retaining until the end of experiment, three rats (33%, 3/9) among displayed spontaneous seizures. These staining sections, from rats managed with antibody, showed abnormal morphology and loss of neuron, increase of apoptosis cell and gliosis.
Conclusions Although further study is needed to clarify the role of Munc-18 in epilepsy, our results show that anti-Munc-18 antibody could induce epilepsy by chronic kindling way, which might link to apoptosis and loss of neuron in hippocampal regions.
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Keywords:Epilepsy;Munc-18;Hippocampus |
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