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Cloning and analysis of a cytochrome P450 gene from rice
Zhu Shifeng 1 #,Cao Xuesong 1,Zhong Yongwang 2,Wei Chunhong 2,Li Yi 2
1.College of Life Science, Peking University
2.College of Life Science- Peking University
*Correspondence author
#Submitted by
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Funding: none
Opened online:25 November 2005
Accepted by: none
Citation: Zhu Shifeng,Cao Xuesong,Zhong Yongwang .Cloning and analysis of a cytochrome P450 gene from rice[OL]. [25 November 2005] http://en.paper.edu.cn/en_releasepaper/content/3792
 
 
Cytochrome P450 mono-oxygenases (P450s) are a superfamily of heme-dependent enzymes which play important roles in almost all of organisms. Based on rice genome database, a gene encoding a rice cytochrome P450 mono-oxygenase was cloned and sequenced (GenBank accession no. AY579214). The coding region of the gene was 1530 nucleotides in length and encoding a protein with 510 amino acid residues in length. Sequence analyses indicated that the cytochrome P450 corresponds to an ent-kaurene oxidase (KO) like protein, which is likely to be involved in phytoanticipins/phytoalexins biosynthesis. Southern blots analysis suggested that there are at least four similar genes encoding ent-kaurene oxidase or ent-kaurene oxidase like proteins in rice genome. Therefore, the gene reported in this paper was named OsKOS1. RT-PCR analysis showed that OsKOS1 was expressed in vegetative organs and reproductive organs, but at a higher level in vegetative organs. The expression level of OsKOS1 in RDV (rice dwarf virus)-infected plants was much lower than that in healthy plants, indicating that OsKOS1 is likely to be involved in defending RDV.
Keywords:cytochrome P450, ent-kaurene oxidase like protein, phytoanticipin/phytoalexin, rice
 
 
 

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