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| Five flavonoids were determined in twco arsenic (As) hyperaccumulators (Pteris multifida and Pteris vittata) and a non-hyperaccumulator (Pteris semipinnata) from uncontaminated and As contaminated sites exposed to different As concentrations under hydroponic conditions by high-performance liquid chromatography (HPLC). The time-course effect of flavonoid induction in P. multifida and P. semipinnata were also studied under 0 and 20 mg As L-1 treatments at different time. There were no significant differences in contents of quercetin, isoquercitrin and kaempferol in the fronds, and also no significant differences in contents of rutin, quercetin, kaempferol and total flavonoids in the roots of the three plants studied under the same As treatment. As for time-course effect, significant differences were observed in contents of rutin, quercetin, hyperin, kaempferol and total flavonoids among times at 20 mg As L-1 treatment. In general, the changes of flavonoid contents in As hyperaccumulators did not directly contribute to As accumulation. |
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| Keywords:Flavonoids;Fern;Arsenic;Hyperaccumulators;HPLC |
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