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In-vitro Inhibitory Effect of Methanol Extracts of Chinese Herbal Drugs on Supercoiling Activity of Bacterial DNA Gyrase
XU Hui 1 *,YAO Pan 2,LIN Guodong 3,CHEN Weiwen 2
1.Research Center of Chinese Herbal Resource Science and Engineering,Guangzhou University of Chinese Medicine
2.Research Center of Chinese Herbal Resource Science and Engineering,Guangzhou University of Chinese Medicine,Guangzhou 510006,China
3.School of Chinese Materia Medica,Guangzhou University of Chinese Medicine,Guangzhou 510006,China
*Correspondence author
#Submitted by
Subject:
Funding: the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Administration of TCM, PRC (No.No. 2005LHR20), Guangdong Natural Science Foundation, PRC (No.No. 06301405)
Opened online: 1 March 2011
Accepted by: none
Citation: XU Hui,YAO Pan,LIN Guodong.In-vitro Inhibitory Effect of Methanol Extracts of Chinese Herbal Drugs on Supercoiling Activity of Bacterial DNA Gyrase[OL]. [ 1 March 2011] http://en.paper.edu.cn/en_releasepaper/content/4411791
 
 
A large number of Chinese herbal drugs (CHDs) exhibit antibacterial activities both in vivo and in vitro, but until now little is known regarding their inhibitory mechanisms. Bacterial DNA gyrase is a proven target for antibacterial agents. Aim of this study was to investigate the in-vitro inhibitory effect of methanol extracts of CHDs against supercoiling activity of bacterial DNA gyrase. Fifteen CHDs were selected and extracted with methanol, respectively. Inhibitory effect of the extracts on DNA gyrase was tested using gel-based DNA supercoiling assay. Among fifteen CHDs tested, methanol extracts of Lonicerae Japonicae Flos (S2), Taraxaci Herba (S7), Glycyrrhizae Radix et Rhizoma Praeparata cum Melle (S8) demonstrated an obvious inhibitory effect against supercoiling activity of DNA gyrase, and the others were either less active or could not be determined with the present method. Moreover, it was likely that S7 and S8 inhibit gyrase in a concentration-dependent manner. In conclusion, DNA supercoiling assay is a promising method to study the inhibitory activity of CHDs on bacterial DNA gyrase. Some CHDs do have gyrase-inhibitory activity as proposed. Further investigations are needed to elucidate the inhibition mechanism of these CHDs on supercoiling activity of gyrase.
Keywords:Chinese herbal drugs; bacterial DNA gyrase; supercoiling activity; inhibitory effect
 
 
 

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