Home > Papers

 
 
Recipient bone marrow-derived cells contribute to neointimal formation after aortic transplantation in rat
Song Zifang *,Zheng Qichang,Liu Shanglong,Hu Shaobo,Li Jun,Xiong Jun,Shang Dan,Zhang Yong,Hu Qinggang
Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
*Correspondence author
#Submitted by
Subject:
Funding: This study was supported by the grants from Specialized Research Fund for the Doctoral Program of Higher Education of Ministry of Education of China (No.No.20070487158), the National Natural Science Foundation of China(No.No.30700798)
Opened online: 2 March 2011
Accepted by: none
Citation: Song Zifang,Zheng Qichang,Liu Shanglong.Recipient bone marrow-derived cells contribute to neointimal formation after aortic transplantation in rat[OL]. [ 2 March 2011] http://en.paper.edu.cn/en_releasepaper/content/4411867
 
 
Objective: To examine the expression of Sry gene in neointimal smooth muscle cells, detect its origin in rat aortic allograft following transplantation. Methods:Sex-mismatched bone marrow transplantation was performed from male Wistar rats to female Wistar rats, and the chimeric rat were prepared. Four weeks later, the rat aortic transplantation model was constituted by means of micro-surgery. The recipients were divided into four groups: male-male aortic isograft group, male-male aortic allograft group, female-chimera aortic allograft group and female-female aortic allograft group. Eight weeks after transplantation, aortic grafts were removed and processed. Histopathological examination and immunohistochemical staining was carried out in aortic sections. α-SMA-positive neointimal cells were harvested from cryostat sections of aortic allograft by microdissection method, and the Sry gene was amplified from the cell DNA by PCR. Results:In all aortic allografts, but not aortic isografts, α-SMA-positive smooth muscle cells were proliferated and accumulated excessively, which resulted in significant neointimal formation and vascular lumen narrowing in aortic grafts. Neointima quantitative assay revealed that the neointimal area and neointimal area/medial area ratio of grafted aorta were significantly higher in all of aortic allograft groups than that of the aortic isograft group (P<0.01), and there was no significant difference between aortic allograft groups (P>0.05). PCR amplification assay indicated that the expression of Sry gene was positive in neointimal smooth muscle cells of aortic allografts in female-chimera and male-male aortic allograft groups respectively, but not in the female-female aortic allograft group. Conclusions:As a source of neointimal smooth muscle cells, recipient bone marrow derived-cells participate in the pathological neointimal hyperplasia and allograft arteriosclerosis in rat. Recipient bone marrow-derived cells may be interesting therapeutic targets for chronic allograft vasculopathy.
Keywords:Transplantation; arteriosclerosis; smooth muscle cells; bone marrow cells
 
 
 

For this paper

  • PDF (0B)
  • ● Revision 0   
  • ● Print this paper
  • ● Recommend this paper to a friend
  • ● Add to my favorite list

    Saved Papers

    Please enter a name for this paper to be shown in your personalized Saved Papers list

Tags

Add yours

Related Papers

Statistics

PDF Downloaded 221
Bookmarked 0
Recommend 7
Comments Array
Submit your papers