| |
| The taxonomic position of a novel strain, designated T7-7T, was investigated. This strain was isolated at 10oC as a diesel-oil-degrading organism from seabed mud in the Bohai Sea. Previously, a polar lipid pattern with phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, and unknown aminolipids AL1 as the primary components was found. Partial 16S rRNA gene sequence analysis indicated that this strain was related most closely to Pusillimonas ginsengisoli DCY25T (98.4% sequence similarity), Pusillimonas soli MJ07T(97.5%) and Pusillimonas noertemannii BN9T (96.7%). The levels of 16S rRNA gene sequence similarity between strain T7-7T and other recognized species of the family Alcaligenaceae were below 95.3%. This suggested that strain T7-7T represented a member of the genus Pusillimonas. Different methods for the construction of phylogenetic dendrograms separated this strain from other previously known strains of Pusillimonas. Fatty acid analysis demonstrated the presence of high concentrations of C16:0 and C17:0 cyclo. Strain T7-7T exhibited relatively low levels of DNA-DNA relatedness with P. ginsengisoli DCY25T(47.1%), P. soli MJ07T(44.5%) and P. noertemannii BN9T (38.6%). Based on the 16S rRNA gene sequence similarity to the strains of Pusillimonas, and other unique phenotypic properties such as fatty acid composition, polar lipid profiles, and DNA-DNA hybridization, T7-7T was classified as a new species with the name Pusillimonas oleiphila sp. nov. The strain type was designated as T7-7T (=CGMCC 1.6148 T =DSM 18250T). |
|
| Keywords:Microbiology; Pusillimonas oleiphila; Fuel oils degradation |
|
