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Objective: Tongue coating appearances have always thought to be related with the progress of gastric caner in Chinese medicine. The genetic polymorphisms in E-cadherin gene (CDH1) may affect invasive/metastatic development of gastric cancer by altering gene transcriptional activity of epithelial cell. The main concern of this paper was to explore the associations among CDH1 gene polymorphisms, tongue coating appearance and predisposition of gastric cancer.
Methods: Four potentially functional polymorphisms (rs13689, rs1801552, rs16260 and rs17690554) of the CDH1 gene were genotyped in a case-control study of 387 incident gastric cancer cases and 392 healthy controls by polymerase chain reaction-ligation detection reaction (PCR-LDR) method and the information of tongue coating was also collected. In order to simplify the tongue diagnosis for easily understood by biomedical professionals, only two typical tongue coating color (white and yellow) and three tongue coating amount (thick, thin and none) were identified for this analysis.
Results: None of the four polymorphisms or their haplotypes achieved significant difference in their distribution between gastric cancer cases and controls. Multiple logistic regression analyses revealed that gastric cancer risk was not significantly associated with the variant genotypes of the four CDH1 polymorphisms as compared with their wild-type genotypes. However, combined the analyses of tongue coating appearance, we found, interestingly, that carrying C allele of rs13689 and G allele of rs17690554 could increase the risk of gastric cancer (OR=2.576, 95%CI=1.227-5.406 for rs13689; OR=2.154, 95%CI=1.014-4.574 for rs17690554, respectively) in individuals with yellow tongue coating.
Conclusions: Our results indicate, for the first time, that genetic variants at CDH1 gene may play a role in gastric cancer preposition in consideration of specific tongue appearance in China. |
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Keywords:Integrative medicine; Tongue coating appearence; Gastric cancer; CDH1 gene |
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