Home > Papers

 
 
Thermokinetic characterization of imipenem hydrolysis with metallo-β-lactamase CcrA from Bacteroides fragilis
Zhai Le #,Yang Kewu *,Liu Chengcheng ,Gao Huizhou,Yang Xia,Shi Ying,Wen Jing #
Northwest University
*Correspondence author
#Submitted by
Subject:
Funding: National Science Foundation of Shaanxi Province (No.2009JM2002), Doctoral Foundation of China (No.200806970005), Key Fund for International Cooperation of Shaanxi Province (No.2010KW-16), National Natural Science Foundation of China (No.20972127)
Opened online: 4 January 2012
Accepted by: none
Citation: Zhai Le ,Yang Kewu ,Liu Chengcheng .Thermokinetic characterization of imipenem hydrolysis with metallo-β-lactamase CcrA from Bacteroides fragilis[OL]. [ 4 January 2012] http://en.paper.edu.cn/en_releasepaper/content/4457650
 
 
Imipenem is a widely used carbapenem antibiotic clinically. In an effort to probe the antibiotic resistance in bacteria, we first report in this paper the thermokinetic parameters of the imipenem hydrolysis with B1 subclass metallo-β-lactamase CcrA from Bacteroides fragilis. The values of activation free energy are 87.540 ± 0.027, 88.781 ± 0.031, 89.901 ± 0.036, and 91.057 ± 0.021 kJomol-1 at 293.15, 298.15, 303.15, and 308.15 K, respectively, the activation enthalpy is 19.099 ± 0.005 kJomol-1, the activation entropy is -233.57 ± 0.09 Jomol-1oK-1 and the apparent activation energy E is 21.598 kJomol-1. The results indicated that the imipenem hydrolysis by CcrA was an exothermic and spontaneous reaction with order of 1.4 and had higher activation enthalpy and lower activation entropy than the cefazolin hydrolysis.
Keywords:Microcalorimetry;Metallo-β-lactamase CcrA;Antibiotic hydrolysis;Imipenem;Thermokinetic parameters
 
 
 

For this paper

  • PDF (0B)
  • ● Revision 0   
  • ● Print this paper
  • ● Recommend this paper to a friend
  • ● Add to my favorite list

    Saved Papers

    Please enter a name for this paper to be shown in your personalized Saved Papers list

Tags

Add yours

Related Papers

Statistics

PDF Downloaded 285
Bookmarked 0
Recommend 5
Comments Array
Submit your papers