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| A novel aptamer-based chemiluminescent (CL) immunoassay coupling with gold nanoparticles (AuNPs) as the tag was developed for the rapid, sensitive detection of platelet-derived growth factor B-chain (PDGF-BB). Typically, PDGF-BB antibodies were immobilized on the surface of 96-well plate to capture target PDGF-BB, and then sandwiched with the aptamer conjugates which were prepared by assembling AuNPs with PDGF-BB aptamer. The captured AuNPs on the 96-well plate was further enlarged in the presence of hydroxylamine (NH2OH) and chloroauric acid (HAuCl4). The thus enlarged AuNPs triggered the reaction between luminol and silver nitrate for the generation of a CL signal. Under the optimal conditions, a good linear relation was achieved in the range of 0.1～1000 pM PDGF-BB with a detection limit down to 10 fM. Other PDGF isoforms (PDGF-AA, PDGF-AB), immunoglobulin G (IgG), immunoglobulin A (IgA), immunoglobulin M (IgM) and interferon α2b (IFN-α2b) showed no obvious interference for the determination of PDGF-BB. Therefore, our strategy provided a simple and sensitive detection of PDGF-BB, and with high specificity, which could find wide applications in protein assay. |
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| Keywords:Aptamer; Chemiluminescence; Gold nanoparticles; PDGF; NH2OH-HAuCl4 |
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