Expression Characteristics of vasa Gene at RNA and Protein Level in Apostichopus japonicus During Gametogenesis

YAN Meng; SUI Juan; SHAO Mingyu; SHENG Wanqiang; ZHANG Zhifeng

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Expression Characteristics of vasa Gene at RNA and Protein Level in Apostichopus japonicus During Gametogenesis

YAN Meng 1 #,SUI Juan 2,SHAO Mingyu 1,SHENG Wanqiang 3,ZHANG Zhifeng 1 *

1.Key Laboratory of Marine Genetics and Breeding, (Ocean University of China), Ministry of Education, Qingdao 266003

2.Institute of Oceanology, Chinese Academy of Science

3.Department of Biological Science, National University of Singapore

*Correspondence author

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Funding: Specialized Research Fund for the Doctoral program of Higher Education, China （No.No. 200804230015）

Opened online:31 January 2012

Accepted by: none

Citation: YAN Meng,SUI Juan,SHAO Mingyu.Expression Characteristics of vasa Gene at RNA and Protein Level in Apostichopus japonicus During Gametogenesis[OL]. [31 January 2012] http://en.paper.edu.cn/en_releasepaper/content/4462753

The expression product of vasa gene is a widely conserved germ line marker and participates in the development regulation of reproductive system. However, no information on vasa gene in Holothuroidea is available. In this paper, we showed the expression characteristics of Apostichopus japonicus vasa gene (Aj-vasa) during gametogenesis in ovary and testis, using in situ hybridization and immunohistochemistry techniques. The expression of Aj-vasa RNA is strongest in early germ cells (oogoniums and spermatogonia), decreases as the maturation proceeded (oocyte and spermatocyte), and absents in spermatid and sperms. Our findings show that the overall expression trend of Aj-vasa RNA is similar with that of its protein（Aj-VASA） during gametogenesis. Nevertheless there was a sexuality difference about the subcellular location of vasa RNA and its protein which was reflected in the specifically expression in cytoplasm of female germ cells and the widely expression in both nucleus cytoplasm of male germ cells. It suggested that Aj-vasa RNA as well as Aj-VASA could be the marker of germ cells during gametogenesis.

Keywords:Apostichopus japonicus; vasa; gonad; gametogenesis; in situ hybridization; immunohistochemistry

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