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In vivo localization of action sites of tenuazonic acid in photosynthetic apparatus by chlorophyll a fluorescence kinetics OJIP
CHEN Shiguo 1 #,ZHANG Min 2,Strasser Reto J?rg 2,QIANG Sheng 3 *
1.College of Life Science, Nanjing Agricultural University, NanJing 210095
2.College of Life Science, Nanjing Agricultural University
3. Laboratory of Bioenergetics and Microbiology, University of Geneva, Switzerland
*Correspondence author
#Submitted by
Subject:
Funding: New Teacher Foundation of Doctoral Program of Education Ministry of China (No.No. 200803071004), 111 Project (No.No. B07030), Foundation of Doctoral Program of Education Ministry of China (No.No. 20090097110018)
Opened online:21 February 2012
Accepted by: none
Citation: CHEN Shiguo,ZHANG Min,Strasser Reto J?rg.In vivo localization of action sites of tenuazonic acid in photosynthetic apparatus by chlorophyll a fluorescence kinetics OJIP[OL]. [21 February 2012] http://en.paper.edu.cn/en_releasepaper/content/4464180
 
 
Tenuazonic acid (TeA), a phytotoxin produced by the fungus Alternaria alternata isolated from diseased Crofton weed (Eupatorium adenophorum), exhibits a strong inhibition in photosynthesis, especially photosystem II (PSII) activity. In vivo the chlorophyll fluorescence induction transient of host plant and in vitro fluorescence transient of six kinds of other higher plants show that TeA acts several sites in photosynthesis apparatus. First, as the classical PSII inhibitors, the most important action site of TeA is that it interrupts electron transport beyond QA on the acceptor side of PSII due to binding to the QB-niche. Moreover, TeA leads to severe inactivation of PSII reaction centers (RCs). On the other hand, TeA has no affect on the antenna pigments, the energy transfer from antenna pigment molecules to RCs, and the oxygen-evolving complex (OEC) at the donor side of PSII. Nevertheless, it's different from the known classical PSII inhibitors that the dominant influence of TeA is not on the primary photochemical reaction but the biochemical reaction after QA. On the basis of the competition experiments with [14C]atrazine, it's further confirmed that TeA does not share with atrazine and DCMU the same binding environment despite their common action target: the QB-site. Additionally, TeA causes inactivation of the FNR system and therefore the reduction of NADP+ at PSI electron acceptor side. These actions distinguish TeA from the classical photosynthesis inhibitors. This provides perhaps a new idea and approach to help the search and development of bioherbicides in tetramic acid families or biomimetic synthesis of new herbicides from TeA precursor.
Keywords:Botany; tenuazonic acid; Chl a fluorescence induction transients; JIP-test; PSII inhibitor; reaction center
 
 
 

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