VEGF is involved in the increase of dermal microvessel permeability induced by tryptase

Bai Qianming; Wang Xinhong; Xu Yali; Yin Lianhua; Li Xiaobo

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VEGF is involved in the increase of dermal microvessel permeability induced by tryptase

Bai Qianming 1,Wang Xinhong 2,Xu Yali 2,Yin Lianhua 2,Li Xiaobo 2 * #

1.Fudan University Shanghai Medical College, ShangHai 200032

2.Fudan University Shanghai Medical College

*Correspondence author

#Submitted by

Subject:

Funding: Specialized Research Fund for the Doctoral Program of higher education（No.200802461083）

Opened online:20 February 2012

Accepted by: none

Citation: Bai Qianming,Wang Xinhong,Xu Yali.VEGF is involved in the increase of dermal microvessel permeability induced by tryptase[OL]. [20 February 2012] http://en.paper.edu.cn/en_releasepaper/content/4464501

Mast cells abundantly reside near the dermal vessels and release tryptase in the condition of cutaneous hypersensitivity and allergy, which is meanifested by rapid edema due to increased vascular permeability. However, the mechanism of mast cell tryptase promoting permeability remains to be defined. In this study, we investigated the effect of tryptase/human mast cells (HMC-1) supernatant on the permeability of human dermal microvascular endothelial cells (HDMECs) and studied whether vascular endothelial growth factor (VEGF) is involved in this effect or not. HMC-1 cells released tryptase pro-degranulating agent a23187 dose-dependently and HMC-1 cells density-dependently. Both tryptase and HMC-1 supernatant can promote permeability of HDMECs dramatically dose-dependently, which was resisted by tryptase inhibitor APC366 (a specific tryptase inhibitor) and partially reversed by a VEGF neutralizing antibody. Tryptase added to HDMECs caused a significant increase of mRNA and protein levels of VEGF, VEGF receptors (Flt-1 and Flk-1) by Real-time RT-PCR assay and Western-blot respectively. Our results suggest that mast cells tryptase can up-regulate the expression of VEGF and VEGF receptors. The VEGF neutralizing antibody can block the dermal microvessel hypermeability induced by tryptase. Thus, VEGF is involved in the increase of tryptse-induced dermal microvessel hypermeability and edema.

Keywords:tryptase; mast cells; VEGF; endothelial cells; permeability; skin

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