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Bioinformatics and expression analysis of the amino acid transporter gene family (OsAAT) in rice
CAI Hongmei *
Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtse River), Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070
*Correspondence author
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Funding: The Specialized Research Found for the Doctoral Program of Higher Education, the Ministry of Education of China (No.No. 20100146120017)
Opened online:20 March 2012
Accepted by: none
Citation: CAI Hongmei.Bioinformatics and expression analysis of the amino acid transporter gene family (OsAAT) in rice[OL]. [20 March 2012] http://en.paper.edu.cn/en_releasepaper/content/4470810
 
 
Nitrogen (N) is one of the most important limiting factors for plant growth and development. Amino acids are the major source of organic N, which is converted from inorganic N absorbed by plant roots from the soil. Amino acid transporters are the principal mediators of organic N distribution and important regulators of resource allocation in plants. Although the complete genomic sequence of rice has already been released, there is still little known about amino acid transporter genes in rice. In this study, 79 OsAAT genes were identified by a database search of the rice genome based upon HMM profiles. A bioinformatics analysis of the complete set of OsAAT genes is presented, including chromosomal location, phylogenetic analysis, gene structure, protein analysis, conserved motifs, protein structures and cis-element analysis of the promoters. In addition, the comprehensive expression profile of OsAAT genes in rice tissues/organs under N starvation conditions was investigated by real-time PCR analysis. Diverse expression patterns of OsAAT genes indicated diverse biological functions of the amino acid transporters and the important roles of OsAAT genes in N uptake, metabolism and distribution during N starvation.
Keywords:nitrogen; OsAAT genes; bioinformatics analysis; expression pattern; rice
 
 
 

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