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Determination of thermokinetic parameters of benzylpenicillin hydrolysis with metallo-β-lactamase CcrA from Bacteroides fragilis
Zhou Lisheng,Zhai Le #,Liu Chengcheng,Shi Ying,Zhou Yajun,Yang Kewu *
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi’an 710069
*Correspondence author
#Submitted by
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Funding: National Science Fund of Shaanxi Province(No.2009JM2002), Shaanxi Provincial Department of Education Fund (No.09JK786), Doctoral Fund of China(No.200806970005), Shaanxi Provincial Bureau of Foreign Expert Fund(No.SLZ2008013), grants (to K.W.Y) from National Science Fund of China(No.20972127), Key Fund for International Cooperation of Shaanxi Province(No.2010KW-16)
Opened online:30 July 2012
Accepted by: none
Citation: Zhou Lisheng,Zhai Le,Liu Chengcheng.Determination of thermokinetic parameters of benzylpenicillin hydrolysis with metallo-β-lactamase CcrA from Bacteroides fragilis[OL]. [30 July 2012] http://en.paper.edu.cn/en_releasepaper/content/4485582
 
 
In this paper, we report the thermokinetic parameters of benzylpenicillin (as the representative of penicillins) hydrolysis with the B1 subclasses MβL CcrA from Bacteroides fragilis. The activation free energy are 87.90±0.03, 88.99±0.01, 89.93±0.04 and 90.93±0.05 kJomol-1 at 293.15, 298.15, 303.15 and 308.15 K. Activation enthalpy is 29.21±0.03 kJomol-1, activation entropy is -200.34±0.08 Jomol-1oK-1, the reaction order is 1.4, and the apparent activation energy E is 31.71 kJomol-1. The summarized thermokinetic data indicated that CcrA prefer to hydrolyze penicillins among three kinds of β-lactam containing antibiotics.
Keywords:Microcalorimetry; Metallo-β-lactamase CcrA; Antibiotic hydrolysis; Benzylpenicillin; Thermokinetic parameters
 
 
 

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