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BnTst-1, A Tourist-like MITE transposon closely associated with genes and may play important roles in gene expression and species evolution in Brassica species
Hou Jinna 1,Long Yan 2 *
1.National Key lab of Crop Genetic Improvement, Huazhong Agricultural University, WuHan 430070
2.National Key lab of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070
*Correspondence author
#Submitted by
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Funding: Specialized Research Fund for the Doctoral Program of Higher Education(New Teachers)(No.20090146120019), National Natural Science Foundation of China (No.No. 30900788 )
Opened online:22 November 2012
Accepted by: none
Citation: Hou Jinna,Long Yan.BnTst-1, A Tourist-like MITE transposon closely associated with genes and may play important roles in gene expression and species evolution in Brassica species[OL]. [22 November 2012] http://en.paper.edu.cn/en_releasepaper/content/4495960
 
 
In this paper, a Tourist-like MITE BnTst-1 was identified in Brassica A and C genome. The BnTst-1 copy number and its distribution in different chromosomes in the genome of three Brassica species were investigated. The sequence variation of BnTst-1 and the TIR and TSD were compared among two Brassica species. The amplification profile diversity caused by BnTst-1 activity intra- and inter- species were confirmed experimentally. DNA methylation level of the BnTst-1 and its flanking sequences, together with EMSA experiments showed that this MITE may play regulation role in gene expression and genome evolution in Brassicaea.
Keywords:Tourist-like MITE; gene expression; evolution; Brassica
 
 
 

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