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Sponsored by the Center for Science and Technology Development of the Ministry of Education
Supervised by Ministry of Education of the People's Republic of China
Development of a single nucleotide polymorphism (SNP) DNA Microarray for Detection and Genotyping of SARS Coronavirus
GUO Xi,LIU Bin * #
TEDA School of Biological Sciences and Biotechnology, Nankai University, Tianjin 300457
*Correspondence author
#Submitted by
Subject:
Funding:
none
Opened online:31 January 2013
Accepted by:
none
Citation: GUO Xi,LIU Bin.Development of a single nucleotide polymorphism (SNP) DNA Microarray for Detection and Genotyping of SARS Coronavirus[OL]. [31 January 2013] http://en.paper.edu.cn/en_releasepaper/content/4517710
A novel coronavirus, severe acute respiratory syndrome coronavirus (SARS-CoV), has been identified as the causative agent of severe acute respiratory syndrome. It has been known that the spike (S) glycoprotein is required for both viral attachment to its permissive cells and fusion of the viral envelope with the host cell membrane. There are 27 single nucleotide variations(SNVs) in the s gene encoding for the spike glycoprotein which are maybe related to the epidemic of this virus[10]. In our study, a SNP detecting microarray system including 108 probes was developed and the utility of this system was demonstrated in the parallel analysis of 25 point mutations from s gene. The efficiency and specificity of this microarray was evaluated by analysis of 20 samples and the veracity was 95%, this result showed that our microarray may be a promising tool for SARS-CoV detection and genotyping and analysis of its prevalence.
Keywords:SARS Coronavirus; Single Nucleotide Polymorphism; DNA Microarray