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miRNA profiling in the sexually mature porcine testes determined by Solexa deep sequencing
Zhou Jiawei 1,Liu Gang 2,Guan Kaifeng 2,Ma Changping 2,Lei Bin 2,Tong Keya 2,Li Jialian 2,Li Fenge 2 * #
1.Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070
2.Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, P.R. China
*Correspondence author
#Submitted by
Subject:
Funding: National Science R&T Program (No.2011BAD28B01), the National Natural Science Foundation of China(No.31072011), Key Projects in Fok Ying Tung Education Foundation(No.131028), Ministry of Education of China (No.20120146110018)
Opened online:11 April 2013
Accepted by: none
Citation: Zhou Jiawei,Liu Gang,Guan Kaifeng.miRNA profiling in the sexually mature porcine testes determined by Solexa deep sequencing[OL]. [11 April 2013] http://en.paper.edu.cn/en_releasepaper/content/4535593
 
 
miRNAs are a large family of small regulatory elements that direct messenger RNA degradation or disrupt mRNA translation. High-throughput Solexa sequencing approach was adopted to expand the repertoire of porcine miRNAs and to explore their roles on spermatogenesis. 237,590 total reads (792 unique reads) representing 65 known mature miRNAs in the miRBase release 14.0 were detected. 116 miRNAs including 12 known miRNAs in the miRBase release 15.0 and 104 novel miRNAs were identified from the un-annotated small RNAs. 466,757 targets for 104 novel miRNAs were predicted by MIREAP. The highly expressed miRNAs in porcine testes (let-7c, let-7f, miR-148a, miR-140* and miR-199b) had a very high rate of SNP mutation, and the most popular SNP was T to G mutation at position 6. Three known miRNAs including miR-514, miR-507, miR-508-5p were significantly differentially expressed between some groups (p<0.05), while two novel miRNAs- DB-m0102 and DB-m0071 were not significantly differentially expressed between all detected groups.
Keywords:Pig; Solexa deep sequencing; miRNA; Spermatogenesis; Testis
 
 
 

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