Duhuo Jisheng Decoction promotes chondrocyte proliferation through accelerated G1/S transition in osteoarthritis

WU Guangwen; CHEN Wenlie; ZHENG Chunsong; CHU Jianfeng; LIN Ruhui; YE Jinxia; XU Huifeng; LI Xihai; HUANG Yunmei; YE Hongzhi; LIU Xianxiang; WU Mingxia

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Duhuo Jisheng Decoction promotes chondrocyte proliferation through accelerated G1/S transition in osteoarthritis

WU Guangwen 1,CHEN Wenlie 2,ZHENG Chunsong 2,CHU Jianfeng 2,LIN Ruhui 3,YE Jinxia 3,XU Huifeng 2,LI Xihai 2,HUANG Yunmei 3,YE Hongzhi 2,LIU Xianxiang 2 #,WU Mingxia 4 *

1.Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, FuZhou 350122

2.Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine

3.Academy of Integrative Medicine Biomedical Research Center, Fujian University of Traditional Chinese Medicine

4.Second Affiliatted People's Hospital of Fujian University of Traditional Chinese Medicine

*Correspondence author

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Subject:

Funding: This study was supported by the Special Research Fund for Doctor Discipline in College （No.20123519110001）

Opened online: 1 July 2013

Accepted by: none

Citation: WU Guangwen,CHEN Wenlie,ZHENG Chunsong.Duhuo Jisheng Decoction promotes chondrocyte proliferation through accelerated G1/S transition in osteoarthritis[OL]. [ 1 July 2013] http://en.paper.edu.cn/en_releasepaper/content/4549063

Objective: To investigate the molecular mechanisms behind the therapeutic effects of Duhuo Jisheng Decoction (DHJSD) on osteoarthritis (OA). Methods: A total of 27 two-month-old male Sprague Dawley rats were randomly divided into three groups: the control group (no papain-induced OA; received an equivalent amount of saline only), the model group (papain-induced OA; received an equivalent amount of saline only) and the DHJSD group [papain-induced OA; received a clinical oral dose of DHJSD (9.3g/kg/day)]. After 8 consecutive weeks of treatment, the morphological changes in articular cartilage were observed under an optical microscopy and by transmission electron microscopy (TEM), and the mRNA and protein expression levels of Cyclin D1, CDK4, CDK6, Rb and p16 were measured by RT-PCR and immunohistochemistry, respectively. Results: Treatment with DHJSD significantly improved the arrangement of articular cartilage structure and collagen fibers and reduced cell degeneration compared with the model group. The mRNA and protein expression levels of Cyclin D1, CDK4, CDK6 and Rb in DHJSD-treated group were significantly increased compared to those in the model group, whereas p16 expression was significantly down-regulated. Conclusion: DHJSD treatment promotes chondrocyte proliferation by promoting the G1/S checkpoint transition in the cell cycle and by up-regulating the expression of Cyclin D1, CDK4, CDK6 and Rb and down-regulating the expression of p16 and this may, in part, explain its clinical efficacy in the treatment of osteoarthritis.

Keywords:Orthopedics of Chinese Medicine; Duhuo Jisheng Decoction (DHJSD); chondrocyte; proliferation; cell cycle; osteoarthritis

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