|
Selection of suitable reference gene is important for accurate measurement of gene expression data obtained using the quantitative real-time PCR (qPCR) method. There has been no systematic investigation of reference genes for porcine chorioallantois which plays important roles in mediating concepts/fetus growth and survival. In this study, six candidate reference genes expressed in pig chorioallantois from Meishan and Yorkshire pigs on gestational days 26 (GD26) and 50 (GD50) were evaluated by qPCR. These genes included TATA box binding protein (TBP), glyceraldehydes-3-phosphate dehydrogenase (GAPDH), ribosomal protein L32 (RPL32), actin-beta (ACTB), ribosomal protein S20 (RPS20) and RNApolymerase polypeptide G (RPG). The expression stability of these genes was investigated by using the GeNorm software program and statistical analyses. The rank order of the expression stability for these genes was (from the most stable to the least stable): TBP, GAPDH, RPL32, ΒACTIN, RPS20 and RPG. Collectively, the results suggest that the TBP and GAPDH could be considered as the suitable reference genes for investigation of gene expression in the pig chorioallantois from the two breeds at different gestational stages. |
|
Keywords:Animal Genetics, Breeding and Reproduction; Reference gene; qPCR; Chorioallantois; Pig |
|