Home > Papers

 
 
Evaluation of reference genes for investigation of gene expression profiles in chorioallantois from Meishan and Yorkshire pigs on gestational days 26 and 50
HOU Chunyan,YU Mei,LI Xiaoping *
Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University
*Correspondence author
#Submitted by
Subject:
Funding: Science Foundation for The Excellent Youth Scholars of Ministry of Education of China (No.No. 20100146120025)
Opened online:30 December 2013
Accepted by: none
Citation: HOU Chunyan,YU Mei,LI Xiaoping.Evaluation of reference genes for investigation of gene expression profiles in chorioallantois from Meishan and Yorkshire pigs on gestational days 26 and 50[OL]. [30 December 2013] http://en.paper.edu.cn/en_releasepaper/content/4577871
 
 
Selection of suitable reference gene is important for accurate measurement of gene expression data obtained using the quantitative real-time PCR (qPCR) method. There has been no systematic investigation of reference genes for porcine chorioallantois which plays important roles in mediating concepts/fetus growth and survival. In this study, six candidate reference genes expressed in pig chorioallantois from Meishan and Yorkshire pigs on gestational days 26 (GD26) and 50 (GD50) were evaluated by qPCR. These genes included TATA box binding protein (TBP), glyceraldehydes-3-phosphate dehydrogenase (GAPDH), ribosomal protein L32 (RPL32), actin-beta (ACTB), ribosomal protein S20 (RPS20) and RNApolymerase polypeptide G (RPG). The expression stability of these genes was investigated by using the GeNorm software program and statistical analyses. The rank order of the expression stability for these genes was (from the most stable to the least stable): TBP, GAPDH, RPL32, ΒACTIN, RPS20 and RPG. Collectively, the results suggest that the TBP and GAPDH could be considered as the suitable reference genes for investigation of gene expression in the pig chorioallantois from the two breeds at different gestational stages.
Keywords:Animal Genetics, Breeding and Reproduction; Reference gene; qPCR; Chorioallantois; Pig
 
 
 

For this paper

  • PDF (0B)
  • ● Revision 0   
  • ● Print this paper
  • ● Recommend this paper to a friend
  • ● Add to my favorite list

    Saved Papers

    Please enter a name for this paper to be shown in your personalized Saved Papers list

Tags

Add yours

Related Papers

Statistics

PDF Downloaded 153
Bookmarked 0
Recommend 5
Comments Array
Submit your papers