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Dynamic expression pattern of Corticotropin releasing hormone and Urotensin I, II gene and responses to acute salinity and temperature challenge in zebrafish during early development
Lei Luo,Aqin Chen,Jianghong Xiong,Congcong Hu,Weiqun Lu * #
College of Fisheries and Life Sciences, Shanghai Ocean University
*Correspondence author
#Submitted by
Subject:
Funding: High Education Commission Grant (No.20113104110002), National Natural Science Foundation of China)
Opened online: 2 January 2014
Accepted by: none
Citation: Lei Luo,Aqin Chen,Jianghong Xiong.Dynamic expression pattern of Corticotropin releasing hormone and Urotensin I, II gene and responses to acute salinity and temperature challenge in zebrafish during early development[OL]. [ 2 January 2014] http://en.paper.edu.cn/en_releasepaper/content/4578577
 
 
Corticotropin releasing hormone (CRH), urotensin I (UI) and urotensin II (UII) are found throughout vertebrate species from fish to human. To further understand the role of CRH, UI and UII in teleosts during development, we investigated the expression pattern of CRH, UI, UIIα and UIIβ, and the response to acute salinity and temperature challenge during the early development of zebra?sh, Danio rerio. The results reveal that CRH, UI, UIIα and UIIβ mRNA are detected from 0hpf, and the relative expression levels alter along with development from 0hpf to 6dpf and reach the highest level at 6dpf except UIIα at 5dpf. After zebrafish at 2, 3, 4 and 5 dpf were exposed to an acute stress for 15min, no significant difference in expression levels of CRH, UI, UIIα and UIIβ mRNA was observed in response to acute osmotic (30ppt) stress compared with control, while cold stress (18℃) and hot stress (38℃) generally inhibited the mRNA levels of CRH, UI, UIIα and UIIβ at each time point apart from at 3dpf. These indicate that the contribution of CRH, UI, UIIα and UIIβ to stress response in zebrafish may be stressor-specific during early development. Overall, results from this study offer new insights into the developmental and temperature-specific function of CRH, UI, UIIα and UIIβ in zebrafish embryos and larvae.
Keywords:Corticotropin releasing hormone; Urotensin; Zebrafish; Development; Salinity; Temperature
 
 
 

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