Extravagant distribution of Izumo1 protein on entire rat sperm surface

Wang Zheng; Yan Yu; Ma Yuanyuan; Chao Lumeng; Xing Wanjin

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Extravagant distribution of Izumo1 protein on entire rat sperm surface

Wang Zheng,Yan Yu,Ma Yuanyuan,Chao Lumeng,Xing Wanjin * #

Department of Biology, School of Life Sciences, Inner Mongolia University, Hohhot 010021

*Correspondence author

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Funding: Research Fund for the Doctoral Program of Higher Education of China （No.20121501110001）, the Natural Science Foundation of Inner Mongolia （No.2012MS0401）

Opened online:17 January 2014

Accepted by: none

Citation: Wang Zheng,Yan Yu,Ma Yuanyuan.Extravagant distribution of Izumo1 protein on entire rat sperm surface[OL]. [17 January 2014] http://en.paper.edu.cn/en_releasepaper/content/4581817

Testis-specific protein Izumo1 is one of the sperm-specific proteins that are involved in sperm-egg fusion, and is essential to mouse sperm-egg fusion. But aside from the limited reports in mouse, its expression, regulation and function had hardly been investigated in a broad range of species. To portray the distribution pattern of rat Izumo1 in testis and sperm, we prepared and purified an anti-rat Izumo1 polyclonal antibody by immunizing Kunming mice with recombinant Trx-Izumo1 fusion protein. Immunohistochemical analysis on the localization of Izumo1 in rat testis and sperm using this antibody revealed that Izumo1 is expressed in cells of nearly all stages of developing sperm cells in the stratified epithelium of the seminiferous tubules from the basement membrane toward the lumen of the tubule. It is mainly localized at the anterior pole of the acrosome of sperm head, however, spreads nearly over the whole membrane including the tail. These results provided data about the spatial and temporal expression pattern of this key gamete fusion protein in the model animal rat.

Keywords:Developmental Biology; Izumo1; rat; testis; sperm; polyclonal antibody

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