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Apolipoprotein E4 Suppresses potassium currents in Hippocampal Neurons
Qin Ying 1 * #,Xie Dongping 2,Weng Yuteng 1,Luo Xiaoli 3,Yang Lifan 4
1.Department of Physiology, School of medicine, Tongji University, Shanghai, China 200092
2. Department of Regenerative Medicine, Tongji University, Shanghai, China 200092
3.School of stomatology, Tongji University, Shanghai, China 200092
4.School of medicine, Tongji University, Shanghai, China 200092
*Correspondence author
#Submitted by
Subject:
Funding: National Natural Science Foundation of China (No.81200832), Specialized Research Fund for the Doctoral Program of Higher Education)
Opened online:20 January 2014
Accepted by: none
Citation: Qin Ying,Xie Dongping,Weng Yuteng.Apolipoprotein E4 Suppresses potassium currents in Hippocampal Neurons[OL]. [20 January 2014] http://en.paper.edu.cn/en_releasepaper/content/4581917
 
 
apolipoprotein E epsilon 4 allele (APOE4) is one of the major risk factors for Alzheimer's disease,but the underlying mechanism remains unclear. Our data show that: 1) application of apoE4 in extracellular solution suppresses the delayed rectifier potassium current (synonym: IK) by 18.9± 2.8 % and 18.1 ± 3.6 % (n=10, P<0.01), at +30 mV and +60 mV of membrane potential. The effect is reversible and shows no voltage-dependency; 2) extracellular application of apoE4 does not show any obvious effect on A-type fast transient potassium current (synonym: IA); 3) application of apoE4 in the intracellular solution suppresses both IA and IK. In case of IK, the reduction rate is 56.2 ± 7.6 % and 58.7 ± 7.4 % (n=10, P<0.01) at +30 mV and +60 mV of membrane potential, respectively; in case of IA, the reductive rate is 58.6 ± 9.7 % and 65.3 ± 8.6 % (n=10, P<0.01) at +30 mV and +60 mV, respectively; 4) the effects of intracellular apoE4 on both IA and IK show obvious voltage-dependent; and 5) in the contrast, application of apoA and apoE3 exhibits no effects on IK or IA. The results indicate that apoE4 molecules could suppress the potassium currents from neurons on both the inner and outer side of neuronal membrane, which support the propulsions that apoE4 expressed by neurons might be responsible for neuronal damages related to the pathogenesis of Alzheimer's disease.
Keywords:patch-clamp; potassium current; apolipoprotein E; Alzheimer's disease
 
 
 

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